INFO @ 20 Dec 2024 06:21:29: [31 MB] # Command line: callpeak -t 2_celltype_fragments/pseudorep1/C2_sorted.tsv -f BED -n C2_pseudoreplicate1 -g hs --outdir 3_peaks -p 0.01 --shift -75 --extsize 150 --nomodel -B --SPMR --keep-dup all --call-summits # ARGUMENTS LIST: # name = C2_pseudoreplicate1 # format = BED # ChIP-seq file = ['2_celltype_fragments/pseudorep1/C2_sorted.tsv'] # control file = None # effective genome size = 2.91e+09 # band width = 300 # model fold = [5, 50] # pvalue cutoff = 1.00e-02 # qvalue will not be calculated and reported as -1 in the final output. # The maximum gap between significant sites is assigned as the read length/tag size. # The minimum length of peaks is assigned as the predicted fragment length "d". # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off # Searching for subpeak summits is on # MACS will save fragment pileup signal per million reads INFO @ 20 Dec 2024 06:21:29: [31 MB] #1 read tag files... INFO @ 20 Dec 2024 06:21:29: [31 MB] #1 read treatment tags... INFO @ 20 Dec 2024 06:21:31: [98 MB] 1000000 reads parsed INFO @ 20 Dec 2024 06:21:33: [99 MB] 2000000 reads parsed INFO @ 20 Dec 2024 06:21:35: [102 MB] 3000000 reads parsed INFO @ 20 Dec 2024 06:21:37: [109 MB] 4000000 reads parsed INFO @ 20 Dec 2024 06:21:38: [112 MB] 5000000 reads parsed INFO @ 20 Dec 2024 06:21:40: [116 MB] 6000000 reads parsed INFO @ 20 Dec 2024 06:21:43: [121 MB] 7000000 reads parsed INFO @ 20 Dec 2024 06:21:44: [125 MB] 8000000 reads parsed INFO @ 20 Dec 2024 06:21:46: [129 MB] 9000000 reads parsed INFO @ 20 Dec 2024 06:21:48: [136 MB] 10000000 reads parsed INFO @ 20 Dec 2024 06:21:50: [142 MB] 11000000 reads parsed INFO @ 20 Dec 2024 06:21:52: [147 MB] 12000000 reads parsed INFO @ 20 Dec 2024 06:21:54: [151 MB] 13000000 reads parsed INFO @ 20 Dec 2024 06:21:56: [156 MB] 14000000 reads parsed INFO @ 20 Dec 2024 06:21:57: [160 MB] 15000000 reads parsed INFO @ 20 Dec 2024 06:21:59: [163 MB] 16000000 reads parsed INFO @ 20 Dec 2024 06:22:01: [168 MB] 17000000 reads parsed INFO @ 20 Dec 2024 06:22:03: [172 MB] 18000000 reads parsed INFO @ 20 Dec 2024 06:22:05: [176 MB] 19000000 reads parsed INFO @ 20 Dec 2024 06:22:07: [178 MB] 20000000 reads parsed INFO @ 20 Dec 2024 06:22:09: [185 MB] 21000000 reads parsed INFO @ 20 Dec 2024 06:22:11: [188 MB] 22000000 reads parsed INFO @ 20 Dec 2024 06:22:13: [192 MB] 23000000 reads parsed INFO @ 20 Dec 2024 06:22:14: [196 MB] 24000000 reads parsed INFO @ 20 Dec 2024 06:22:16: [200 MB] 25000000 reads parsed INFO @ 20 Dec 2024 06:22:18: [204 MB] 26000000 reads parsed INFO @ 20 Dec 2024 06:22:20: [208 MB] 27000000 reads parsed INFO @ 20 Dec 2024 06:22:22: [213 MB] 28000000 reads parsed INFO @ 20 Dec 2024 06:22:24: [217 MB] 29000000 reads parsed INFO @ 20 Dec 2024 06:22:26: [221 MB] 30000000 reads parsed INFO @ 20 Dec 2024 06:22:27: [224 MB] 31000000 reads parsed INFO @ 20 Dec 2024 06:22:29: [230 MB] 32000000 reads parsed INFO @ 20 Dec 2024 06:22:30: [231 MB] #1 tag size is determined as 1 bps INFO @ 20 Dec 2024 06:22:30: [231 MB] #1 tag size = 1.0 INFO @ 20 Dec 2024 06:22:30: [231 MB] #1 total tags in treatment: 32553324 INFO @ 20 Dec 2024 06:22:30: [231 MB] #1 finished! INFO @ 20 Dec 2024 06:22:30: [231 MB] #2 Build Peak Model... INFO @ 20 Dec 2024 06:22:30: [231 MB] #2 Skipped... INFO @ 20 Dec 2024 06:22:30: [231 MB] #2 Use 150 as fragment length INFO @ 20 Dec 2024 06:22:30: [231 MB] #2 Sequencing ends will be shifted towards 5' by 75 bp(s) INFO @ 20 Dec 2024 06:22:30: [231 MB] #3 Call peaks... INFO @ 20 Dec 2024 06:22:30: [231 MB] #3 Going to call summits inside each peak ... INFO @ 20 Dec 2024 06:22:30: [231 MB] #3 Call peaks with given -log10pvalue cutoff: 2.00000 ... INFO @ 20 Dec 2024 06:22:30: [231 MB] #3 Pre-compute pvalue-qvalue table... INFO @ 20 Dec 2024 06:23:35: [933 MB] #3 In the peak calling step, the following will be performed simultaneously: INFO @ 20 Dec 2024 06:23:35: [933 MB] #3 Write bedGraph files for treatment pileup (after scaling if necessary)... C2_pseudoreplicate1_treat_pileup.bdg INFO @ 20 Dec 2024 06:23:35: [933 MB] #3 Write bedGraph files for control lambda (after scaling if necessary)... C2_pseudoreplicate1_control_lambda.bdg INFO @ 20 Dec 2024 06:23:35: [933 MB] #3 --SPMR is requested, so pileup will be normalized by sequencing depth in million reads. INFO @ 20 Dec 2024 06:23:35: [933 MB] #3 Call peaks for each chromosome... INFO @ 20 Dec 2024 06:24:49: [933 MB] #4 Write output xls file... 3_peaks/C2_pseudoreplicate1_peaks.xls INFO @ 20 Dec 2024 06:24:50: [933 MB] #4 Write peak in narrowPeak format file... 3_peaks/C2_pseudoreplicate1_peaks.narrowPeak INFO @ 20 Dec 2024 06:24:50: [933 MB] #4 Write summits bed file... 3_peaks/C2_pseudoreplicate1_summits.bed INFO @ 20 Dec 2024 06:24:50: [933 MB] Done!