FastQCFastQC Report
Thu 12 May 2016
GD01-trimmed-pair1.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename GD01-trimmed-pair1.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 37450600
Filtered Sequences 0
Sequence length 21-150
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 51612 0.13781354637842919 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 35963280 11.334372 18.07204 135-139
TTTTT 50527525 6.069385 8.671839 10-14
AAAAA 40972685 4.017237 4.6722207 125-129
TGTTT 10070110 1.467017 5.6868305 2
TTTGA 9409805 1.3162696 5.4398274 4
GTTTG 7278160 1.2858988 6.404611 3
GTGTT 5733425 1.0129765 6.181457 1
Produced by FastQC (version 0.10.1)