##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	GD01-trimmed-pair1.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	37450600	
Filtered Sequences	0	
Sequence length	21-150	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.01822849300145	32.0	27.0	32.0	27.0	32.0
2	31.36347115934057	32.0	32.0	32.0	27.0	32.0
3	31.478620876568066	32.0	32.0	32.0	32.0	32.0
4	31.435186592471148	32.0	32.0	32.0	32.0	32.0
5	31.389184338835694	32.0	32.0	32.0	32.0	32.0
6	35.22735197833946	37.0	37.0	37.0	32.0	37.0
7	35.12439143832142	37.0	37.0	37.0	32.0	37.0
8	35.0388159335231	37.0	37.0	37.0	32.0	37.0
9	35.021692976881546	37.0	37.0	37.0	32.0	37.0
10-14	34.94880653981512	37.0	37.0	37.0	32.0	37.0
15-19	34.83175712004614	37.0	37.0	37.0	30.0	37.0
20-24	34.73668617581107	37.0	37.0	37.0	27.0	37.0
25-29	34.78647467003556	37.0	37.0	37.0	31.0	37.0
30-34	34.651188645452486	37.0	37.0	37.0	28.0	37.0
35-39	34.50404301365419	37.0	37.0	37.0	27.0	37.0
40-44	34.38759021025292	37.0	37.0	37.0	27.0	37.0
45-49	34.27355117628183	37.0	37.0	37.0	27.0	37.0
50-54	34.157750276639604	37.0	37.0	37.0	27.0	37.0
55-59	34.057226440944724	37.0	37.0	37.0	27.0	37.0
60-64	33.940734818586336	37.0	37.0	37.0	26.0	37.0
65-69	33.829807894588825	37.0	37.0	37.0	22.0	37.0
70-74	33.725450103274135	37.0	37.0	37.0	22.0	37.0
75-79	33.63115762488606	37.0	37.0	37.0	22.0	37.0
80-84	33.51536207057017	37.0	37.0	37.0	22.0	37.0
85-89	33.425547376386014	37.0	37.0	37.0	22.0	37.0
90-94	33.32185922439197	37.0	37.0	37.0	22.0	37.0
95-99	33.19493763116172	37.0	37.0	37.0	16.6	37.0
100-104	33.07076708149557	37.0	36.0	37.0	13.0	37.0
105-109	32.96946290842492	37.0	32.0	37.0	13.0	37.0
110-114	32.8423774991178	37.0	32.0	37.0	13.0	37.0
115-119	32.66915869142255	37.0	32.0	37.0	13.0	37.0
120-124	32.50751399882634	37.0	32.0	37.0	13.0	37.0
125-129	32.35100562490505	37.0	32.0	37.0	13.0	37.0
130-134	32.19087827924575	37.0	32.0	37.0	13.0	37.0
135-139	32.06691962062943	37.0	32.0	37.0	13.0	37.0
140-144	31.961687439710875	37.0	32.0	37.0	13.0	37.0
145-149	32.218807906925484	37.0	32.0	37.0	14.8	37.0
150	34.36630258833323	37.0	32.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	30.0
12	377.0
13	2332.0
14	10211.0
15	29816.0
16	60120.0
17	96492.0
18	139675.0
19	195224.0
20	259569.0
21	321858.0
22	366892.0
23	399738.0
24	427121.0
25	458244.0
26	499949.0
27	562298.0
28	651695.0
29	775901.0
30	962762.0
31	1265196.0
32	1801417.0
33	2903389.0
34	5611591.0
35	1.142696E7
36	7748949.0
37	472788.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	23.25546488135557	39.05522718714219	17.283588700888917	20.40571923061332
2	22.37162587136466	31.83754490415973	28.794479767277398	16.99634945719821
3	21.350624166301653	30.604653127549692	26.937787585543365	21.106935120605293
4	21.010864979466284	30.0561272716592	27.851110529604334	21.081897219270186
5	21.523964877938326	30.232411900123722	27.996168813075702	20.24745440886225
6	22.183080076412775	28.031814496833285	28.765556813114156	21.01954861363978
7	23.079431503388367	28.722564904595053	27.571924912919943	20.626078679096636
8	21.11354070928364	28.331367165658484	29.04296588160591	21.512126243451966
9	21.654755562372312	27.946336105796792	28.98250925602336	21.416399075807536
10-14	21.911627597034013	28.383754977359366	28.528220654459414	21.176396771147203
15-19	22.009252529177875	28.25378690440898	28.44537829397015	21.291582272442987
20-24	22.14522156730206	28.244296452192707	28.17148281743693	21.4389991630683
25-29	22.286502063015362	28.230587742948448	28.03020205144216	21.452708142594023
30-34	22.350319985160077	28.23840883822899	27.88223585152491	21.529035325086024
35-39	22.35036819121267	28.27133149756621	27.854595187964126	21.523705123256992
40-44	22.452343232376908	28.332282023187478	27.78325751665826	21.432117227777354
45-49	22.48177419828478	28.325102991650443	27.698040335125707	21.49508247493907
50-54	22.52938497844264	28.319937193162037	27.696370281132136	21.45430754726319
55-59	22.575215118197093	28.415666716799425	27.602765106360362	21.40635305864312
60-64	22.648605115218675	28.388077958363567	27.530158782334368	21.43315814408339
65-69	22.645868397795084	28.405261507411872	27.551642476252496	21.397227618540544
70-74	22.681859776235324	28.53646689758665	27.43411283956157	21.347560486616455
75-79	22.787160980211997	28.44020278618572	27.407516451647506	21.365119781954775
80-84	22.79147164180667	28.507457999567375	27.351619280753837	21.34945107787212
85-89	22.81141912271417	28.581057357924728	27.31351806916092	21.29400545020018
90-94	22.889002295218752	28.50936658620014	27.267870188085208	21.333760930495895
95-99	22.88623498040199	28.564925703348372	27.238667436093962	21.310171880155675
100-104	22.872275399929087	28.671313657682763	27.171623788694088	21.28478715369406
105-109	22.948295752525443	28.634653783914228	27.112642068306346	21.304408395253986
110-114	22.94422806358464	28.705058575017095	27.09494849189838	21.255764869499888
115-119	23.012902398532116	28.75457715871612	27.028677392168653	21.203843050583103
120-124	22.978100018889688	28.791371824484102	27.0210304896363	21.209497666989908
125-129	23.017705045118202	28.775566800985814	27.00613565123835	21.200592502657635
130-134	23.04837863921294	28.813040825006908	26.937813153977974	21.20076738180218
135-139	23.077017160824923	28.761783740006276	26.950282814272974	21.21091628489583
140-144	23.04183269589117	28.75207812006575	26.959166578881423	21.24692260516166
145-149	23.041165140995684	28.56220577367536	27.08168054611832	21.314948539210636
150	24.058894568663668	24.18479249218054	28.73898326310359	23.0173296760522
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.6611841119689
2	39.36797532856287
3	42.45755928690694
4	42.092762198736466
5	41.77141928680058
6	43.202628690052556
7	43.70551018248501
8	42.62566695273561
9	43.07115463817985
10-14	43.08802436818122
15-19	43.300834801620866
20-24	43.58422073037036
25-29	43.73921020560939
30-34	43.8793553102461
35-39	43.874073314469655
40-44	43.88446046015426
45-49	43.97685667322385
50-54	43.983692525705834
55-59	43.98156817684021
60-64	44.081763259302065
65-69	44.04309601633563
70-74	44.02942026285178
75-79	44.152280762166775
80-84	44.14092271967879
85-89	44.10542457291435
90-94	44.22276322571465
95-99	44.196406860557666
100-104	44.15706255362315
105-109	44.252704147779426
110-114	44.19999293308453
115-119	44.21674544911522
120-124	44.187597685879595
125-129	44.21829754777583
130-134	44.249146021015115
135-139	44.28793344572075
140-144	44.28875530105283
145-149	44.35611368020632
150	47.07622424471587
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	72308.0
1	56678.0
2	45739.0
3	69654.0
4	72721.0
5	42331.5
6	24303.0
7	18692.0
8	15798.5
9	14025.0
10	13151.5
11	12774.0
12	12504.5
13	12625.5
14	13196.0
15	14214.0
16	15813.5
17	18588.5
18	23243.0
19	30573.0
20	40955.0
21	55507.5
22	75356.0
23	102000.5
24	137931.0
25	178604.5
26	224246.5
27	279727.5
28	341663.0
29	406999.5
30	477042.5
31	548964.5
32	624964.0
33	711588.0
34	805570.5
35	910914.0
36	1011483.0
37	1121296.5
38	1249418.0
39	1347270.5
40	1428293.0
41	1498195.5
42	1550330.5
43	1596449.5
44	1627400.0
45	1635456.0
46	1620937.0
47	1554159.5
48	1467431.5
49	1416143.0
50	1320209.0
51	1207671.0
52	1119877.5
53	1017340.0
54	909503.0
55	808746.5
56	721840.5
57	646127.0
58	585834.0
59	526928.5
60	452668.0
61	375178.0
62	302052.0
63	236044.5
64	184573.5
65	143773.5
66	109901.5
67	79638.5
68	55911.5
69	39312.0
70	27540.5
71	20158.0
72	16142.5
73	13432.5
74	11348.0
75	10011.0
76	9050.0
77	8374.0
78	7912.0
79	7431.0
80	6907.5
81	6405.0
82	5932.5
83	5518.5
84	5099.5
85	4655.5
86	4380.0
87	4275.5
88	4167.0
89	3892.5
90	3608.5
91	3508.0
92	3598.5
93	3800.0
94	4454.5
95	11036.0
96	17815.5
97	16481.5
98	10321.0
99	4939.0
100	2276.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00663807789461317
2	1.3350920946526892E-5
3	1.0680736757221513E-5
4	0.0
5	1.735619723048496E-4
6	0.013428356288016748
7	0.00384773541678905
8	0.0011214773595082587
9	0.0010467122022077082
10-14	8.555270142534433E-4
15-19	4.528632385061922E-4
20-24	5.23359946934292E-4
25-29	0.0011140484630093933
30-34	8.84465398749555E-4
35-39	0.006899356381286083
40-44	0.006671777760288427
45-49	0.0016628134132696006
50-54	0.005218905769851411
55-59	0.0030017744720332797
60-64	0.001204242733572293
65-69	0.006773350295101216
70-74	0.003171270129950086
75-79	0.0010765550631996763
80-84	0.003953803133991118
85-89	0.003246386745851306
90-94	0.00129198164057175
95-99	0.004737124596975897
100-104	0.00994494646442484
105-109	0.005919185300918337
110-114	0.003284873317194844
115-119	0.009592187633061381
120-124	0.008252471359229291
125-129	0.0020838414855238914
130-134	0.005319450193428679
135-139	0.015504349602917826
140-144	0.00747388746337387
145-149	0.004187204307908455
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
20-24	923.0
25-29	1969.0
30-34	3941.0
35-39	7232.0
40-44	11589.0
45-49	17281.0
50-54	24158.0
55-59	32678.0
60-64	42625.0
65-69	54591.0
70-74	66783.0
75-79	81261.0
80-84	97239.0
85-89	114070.0
90-94	133206.0
95-99	153287.0
100-104	176070.0
105-109	201467.0
110-114	227823.0
115-119	256243.0
120-124	289523.0
125-129	328465.0
130-134	379883.0
135-139	457072.0
140-144	659919.0
145-149	5587154.0
150-151	2.8044148E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	55.942172358747214
#Duplication Level	Relative count
1	100.0
2	31.85102778174692
3	14.025597583130907
4	8.26205882953316
5	5.6063606115658615
6	3.986609238808712
7	3.20173916593521
8	2.460756496356325
9	2.061687317561085
10++	32.67162015962767
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51612	0.13781354637842919	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGGGG	35963280	11.334372	18.07204	135-139
TTTTT	50527525	6.069385	8.671839	10-14
AAAAA	40972685	4.017237	4.6722207	125-129
TGTTT	10070110	1.467017	5.6868305	2
TTTGA	9409805	1.3162696	5.4398274	4
GTTTG	7278160	1.2858988	6.404611	3
GTGTT	5733425	1.0129765	6.181457	1
>>END_MODULE