FastQCFastQC Report
Wed 15 Feb 2017
OU_LexBC016_S56_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOU_LexBC016_S56_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37544
Sequences flagged as poor quality0
Sequence length35-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGC770.20509269124227575No Hit
GGGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTG740.1971020669081611No Hit
GTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGCA530.14116769656935862No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG410.10920519923290006No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGATG150.00219248470.1239966
TATGTCC401.595552E-460.50232770
GGGAGTG200.007148667752.0326
TATGGTT350.007201490551.85913570
TTATGTC751.2513829E-648.40186370
ATTATGT854.2860847E-836.32885469
GAGTATG700.002514199434.57275470
TATTATG901.2392411E-732.20798568
GGTTTGG1057.015642E-726.4289511
GTTTGGT1052.1148462E-523.1253342
GGGGGGT750.00189659623.1253342
GAAAGCG1403.395926E-722.29942737
AAAGCGG1403.395926E-722.29942738
AGCGGCT1605.0569724E-821.6840
ATTATTA1304.0974337E-621.5766166
ACATTCA1454.7616413E-721.53048553
AAGCGGC1454.7616413E-721.53048539
ATGTCTG1454.7616413E-721.53048526
TGTGCAG1454.7616413E-721.53048546
TTGGTGG1454.7616413E-721.5304854
Produced by FastQC (version 0.11.2)