FastQCFastQC Report
Wed 15 Feb 2017
OU_LexBC016_S56_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOU_LexBC016_S56_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39047
Sequences flagged as poor quality0
Sequence length28-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTG760.1946372320536789No Hit
GGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGC680.17414910236381798No Hit
GTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGCA480.12292877813916561No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGGT150.002280303769.4397426
GAGCGGC200.00712488652.0798074
TTGGTTG200.00712488652.0798073
GTCCCAC200.00712488652.07980747
CCTCTGC200.00712488652.07980731
TATGTCC600.001293532939.5928470
AGTATGG902.3461104E-432.9940370
AAAAAGG750.00386642431.67426770
GGTGTGG450.00442888530.862114
TAAGCAT450.00442888530.8621137
GAAATTT851.3492648E-424.5081449
GAGTATG1000.005983959423.75570370
TTGGTGG750.001887845223.146585
GTCTGTG1055.634728E-419.83992827
AAAGTCT1055.634728E-419.83992848
TTTGGTG900.00543449619.2888184
GGCGTTA1107.7071734E-418.9381127
TGTGGAA1107.7071734E-418.93811232
CCAAAGT1107.7071734E-418.93811246
CCCATGA1107.7071734E-418.93811216
Produced by FastQC (version 0.11.2)