FastQCFastQC Report
Wed 15 Feb 2017
OU_LexBC016_S56_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOU_LexBC016_S56_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21090
Sequences flagged as poor quality0
Sequence length20-76
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGC420.1991465149359886No Hit
GGGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTG420.1991465149359886No Hit
GTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGCA380.18018018018018017No Hit
TTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGCAG260.12328117591275486No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTGG150.002273103769.382422
CACCGCC150.002273103769.3824244
TTGGGCC150.002273103769.382424
TTATGTC452.110783E-456.9395770
ATTAGTC200.007102676752.0368244
TCAGACG200.007102676752.0368213
TATTATG400.002091675535.8845268
TTGACCA400.002442396534.7738159
ATTATGT500.004995875530.0359969
ACAACTT500.00736506827.75296854
GAAAGCG500.00736506827.75296837
GGAAACA500.00736506827.75296850
GGAAATT901.9569333E-423.1274729
TATTATT1000.00960990717.42840265
Produced by FastQC (version 0.11.2)