FastQCFastQC Report
Wed 15 Feb 2017
OU_LexBC013_S53_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOU_LexBC013_S53_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27242
Sequences flagged as poor quality0
Sequence length24-76
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGC620.22758975111959473No Hit
GGGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTG590.21657734380735627No Hit
GTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGCA510.18721092430805372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGTCC452.4873752E-670.9924670
ATTATGT656.4604374E-439.318970
TTATGTC602.7853792E-432.0552769
ACCCGCT652.1752721E-531.98274627
CCCGCTG652.1752721E-531.98274628
CGCTGAA553.0961106E-431.4981630
CCGCTGA553.0961106E-431.4981629
GGCGACC553.0961106E-431.4981623
CGTGGCG553.0961106E-431.4981620
TCAGCGG553.0961106E-431.4981651
ATCAGAC553.0961106E-431.4981614
AGACGTG553.0961106E-431.4981617
TCAGATC553.0961106E-431.4981610
AGCGGAG553.0961106E-431.4981653
TCAGACG553.0961106E-431.4981615
CTCAGAT553.0961106E-431.498169
CAGACGT553.0961106E-431.4981616
TGGCGAC553.0961106E-431.4981622
CAGCGGA553.0961106E-431.4981652
GTCAGCG553.0961106E-431.4981650
Produced by FastQC (version 0.11.2)