FastQCFastQC Report
Wed 15 Feb 2017
OU_LexBC013_S53_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOU_LexBC013_S53_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28007
Sequences flagged as poor quality0
Sequence length23-76
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTG780.27850180312064843No Hit
GGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGC610.2178026921840968No Hit
GTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGCA540.19280894062198736No Hit
GGGGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGT290.1035455421858821No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAACT200.00712731552.0366155
GTGGTGG350.001289933539.646945
GCTGAAT500.007406458327.75285731
GGGTTTG658.1788906E-426.685441
TTGGTGG1107.6755485E-418.9224034
GGTTTGG950.007410203618.258461
GTTTGGT1000.00995876517.3455352
TGGTGGA1200.001376756617.3455355
TGGAAAT1250.00180956416.6517148
GGTGGAA1250.00180956416.6517146
GATGTCT1300.00235192516.01126325
GTTATGA1300.00235192516.01126320
GGAAATT1300.00235192516.0112639
TGATGTC1300.00235192516.01126324
TTATGAT1350.003025337115.41825421
GAAATTT1400.003854358314.86760210
TGTTATG1450.004866887314.35492619
ATGTCTG1450.004866887314.35492626
GAAAGCG1450.004866887314.35492637
GGAAAGC1450.004866887314.35492636
Produced by FastQC (version 0.11.2)