FastQCFastQC Report
Wed 15 Feb 2017
OU_LexBC010_S50_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOU_LexBC010_S50_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55167
Sequences flagged as poor quality0
Sequence length15-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA2460.44591875577791074No Hit
GGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGC860.15589029673536717No Hit
AGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA840.15226494099733537No Hit
GGGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTG800.14501422952127177No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA780.14138887378324No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA780.14138887378324No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAAA740.1341381623071764No Hit
GTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGCA700.1268874508311128No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA630.11419870574800152No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA630.11419870574800152No Hit
TCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA570.10332263853390614No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCACG150.002295668769.3608440
CGTGACC150.002295668769.360841
TTCACGT150.002295668769.3608441
TCCACGG150.002295668769.3608432
CGGACTT150.002295668769.3608436
CACGGAC200.007172681452.02062634
CAACATT200.007172681452.0206268
GAGGTAA200.007172681452.02062631
TTCCACG200.007172681452.02062631
ACGGACT200.007172681452.02062635
ACGTCAT200.007172681452.02062644
CACGTCA200.007172681452.02062643
TCACGTC200.007172681452.02062642
ACTATCT503.890557E-450.58181870
TTGTAGC306.100237E-446.2405617
TGCGACC306.100237E-446.240561
CCACAGG306.100237E-446.2405623
GTAGCCA350.001302859939.63476619
CAGGCTT350.001302859939.63476626
TGTAGCC350.001302859939.63476618
Produced by FastQC (version 0.11.2)