FastQCFastQC Report
Wed 15 Feb 2017
OU_LexBC010_S50_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOU_LexBC010_S50_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56395
Sequences flagged as poor quality0
Sequence length44-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA3020.5355084670626828No Hit
GGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGC900.1595886160120578No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA870.15426899547832254No Hit
AGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA820.14540296125543045No Hit
GGGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTG790.14008334072169518No Hit
GTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGCA650.1152584448975973No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAAA650.1152584448975973No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA600.10639241067470522No Hit
TCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA600.10639241067470522No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAAAAAAAAAAAAAA580.10284599698554837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGA252.972114E-669.325641
TGACCAG200.00718789751.99423239
CCTCCCC200.00718789751.99423229
TCGCGAC404.8060472E-543.3285262
ATTATGT1053.3062973E-431.15976770
CGCGACC1800.026.9599721
GCGACCT2750.025.2093242
GCGACCC3650.024.69132422
AGCGGAG3650.024.69132451
CGACCCG3700.024.35765823
GTGGCGA3700.024.35765819
ACCCGCT3700.024.35765825
CAGCGGA3600.024.07140450
CCGCTGA3600.024.07140427
CGTGGCG3750.024.0328918
ATCAGAC3750.024.0328912
TGGCGAC3750.024.0328920
TAGTCAG3750.024.0328946
CTCAGAT3750.024.032897
CAGACGT3750.024.0328914
Produced by FastQC (version 0.11.2)