OU_LexBC010_S50_L001_R1_001.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	OU_LexBC010_S50_L001_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27648
Sequences flagged as poor quality	0
Sequence length	53-76
%GC	41

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	312	1.128472222222222	No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAAA	66	0.2387152777777778	No Hit
AGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	61	0.220630787037037	No Hit
TCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	59	0.21339699074074073	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	56	0.20254629629629628	No Hit
GGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGC	54	0.1953125	No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	52	0.1880787037037037	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAAAAAAAAAAAAAA	48	0.1736111111111111	No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	38	0.13744212962962962	No Hit
GGGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTG	37	0.13382523148148148	No Hit
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	35	0.12659143518518517	No Hit
GTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGCA	28	0.10127314814814814	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATTACG	15	1.1196521E-4	147.61775	70
ATTATGT	50	1.9602085E-6	73.808876	70
CATTCAT	15	0.0022634363	69.51454	60
GTTGGCA	15	0.0022963423	69.26268	55
ATTCATA	20	0.0069710175	52.326183	61
GCATTCA	20	0.0071235667	52.04129	59
TTACTGC	20	0.007174964	51.94701	41
AAATTAC	20	0.007174964	51.94701	38
GACATGT	20	0.007174964	51.94701	50
GGCTTAA	20	0.007174964	51.94701	10
ATTACTG	30	6.0893636E-4	46.17512	40
TGCGACC	35	0.0013005849	39.578674	1
AATTACT	35	0.0013005849	39.578674	39
CGCGACC	95	1.9281288E-10	36.45404	1
TATTATG	55	1.613367E-4	35.179424	69
GCGACCT	150	0.0	34.631344	2
GTGGGTG	40	0.0025057234	34.63134	7
CGACCTC	160	0.0	34.63134	3
GACCTCA	175	0.0	31.66294	4
ATTGTTA	70	3.446549E-5	29.900679	61