##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	OU_LexBC008_S48_L004_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5917
Sequences flagged as poor quality	0
Sequence length	54-76
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24218353895555	32.0	32.0	32.0	32.0	32.0
2	30.897583234747337	32.0	32.0	32.0	32.0	32.0
3	30.99526787223255	32.0	32.0	32.0	32.0	32.0
4	30.848233902315364	32.0	32.0	32.0	32.0	32.0
5	30.555179989859727	32.0	32.0	32.0	32.0	32.0
6	33.64340037181004	36.0	36.0	36.0	32.0	36.0
7	33.68987662666892	36.0	36.0	36.0	32.0	36.0
8	33.95149569038364	36.0	36.0	36.0	32.0	36.0
9	33.74311306405273	36.0	36.0	36.0	32.0	36.0
10-11	33.800152104106814	36.0	36.0	36.0	32.0	36.0
12-13	33.46087544363698	36.0	36.0	36.0	27.0	36.0
14-15	33.118556701030926	36.0	34.0	36.0	24.0	36.0
16-17	32.848318404596924	36.0	32.0	36.0	20.5	36.0
18-19	32.84122021294575	36.0	32.0	36.0	14.0	36.0
20-21	32.95834037519013	36.0	32.0	36.0	21.0	36.0
22-23	33.09371303025182	36.0	36.0	36.0	21.0	36.0
24-25	32.55957410850093	36.0	34.0	36.0	14.0	36.0
26-27	32.337248605712354	36.0	32.0	36.0	14.0	36.0
28-29	31.87451411188102	36.0	32.0	36.0	14.0	36.0
30-31	31.312658441777927	36.0	29.5	36.0	14.0	36.0
32-33	30.41499070474903	36.0	27.0	36.0	14.0	36.0
34-35	30.543518675004222	36.0	29.5	36.0	14.0	36.0
36-37	30.849754943383473	36.0	29.5	36.0	14.0	36.0
38-39	29.895301673145177	34.0	27.0	36.0	14.0	36.0
40-41	28.75891499070475	32.0	21.0	36.0	14.0	36.0
42-43	29.26068953861754	34.0	24.0	36.0	14.0	36.0
44-45	29.995436876795672	36.0	27.0	36.0	14.0	36.0
46-47	29.962734493831334	36.0	27.0	36.0	14.0	36.0
48-49	30.339783674159204	36.0	27.0	36.0	14.0	36.0
50-51	29.919891837079604	36.0	27.0	36.0	14.0	36.0
52-53	29.698411357106643	36.0	27.0	36.0	14.0	36.0
54-55	30.05653785410827	36.0	27.0	36.0	14.0	36.0
56-57	30.001456085392736	36.0	24.0	36.0	14.0	36.0
58-59	29.68069014568065	36.0	24.0	36.0	14.0	36.0
60-61	28.67298110605585	36.0	14.0	36.0	14.0	36.0
62-63	28.212120748398014	32.0	14.0	36.0	14.0	36.0
64-65	27.76008974548575	32.0	14.0	36.0	14.0	36.0
66-67	27.72066191057544	32.0	14.0	36.0	14.0	36.0
68-69	27.18771840447159	32.0	14.0	36.0	14.0	36.0
70-71	26.781962092108543	32.0	14.0	36.0	14.0	36.0
72-73	25.93200063908347	29.5	14.0	36.0	14.0	36.0
74-75	25.039974896710493	27.0	14.0	36.0	14.0	36.0
76	25.240118997025075	27.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
11410	1	-0.34818912995382334
11410	2	0.07971617236070472
11410	3	-0.11038422616637789
11410	4	0.2468501888881498
11410	5	0.2581356107846098
11410	6	0.026768269617654994
11410	7	0.7728677793001424
11410	8	-0.6768623791302062
11410	9	0.035106128745319154
11410	10-11	0.6300355300504563
11410	12-13	-0.23485284611732737
11410	14-15	-0.9884598444846162
11410	16-17	0.4659940369287341
11410	18-19	0.15256541829348436
11410	20-21	-0.5590131174349295
11410	22-23	-0.5002965844698508
11410	24-25	-1.5696269436664956
11410	26-27	-0.9884683607837275
11410	28-29	-1.0526114892421603
11410	30-31	-2.380231012212672
11410	32-33	-1.7520831896688591
11410	34-35	-0.43330849254611437
11410	36-37	-0.7824638522761838
11410	38-39	-2.831395983538016
11410	40-41	-1.5299706662569967
11410	42-43	-1.4613786285934047
11410	44-45	-0.874893758131396
11410	46-47	-2.678546849325752
11410	48-49	-2.199478975941492
11410	50-51	0.022783665686215926
11410	52-53	-1.514341824044891
11410	54-55	-2.1317082575883184
11410	56-57	0.04300758269676308
11410	58-59	-0.8605808946519531
11410	60-61	0.723689990762793
11410	62-63	-0.4192186988107238
11410	64-65	-0.9979991765573786
11410	66-67	0.8630972202474823
11410	68-69	-1.6040734247542794
11410	70-71	-2.282814273576939
11410	72-73	-2.1517616986156654
11410	74-75	-3.4693698248041294
11410	76	-3.7220657273890367
11411	1	0.08985842949548584
11411	2	0.16982881316170761
11411	3	0.8896157738336221
11411	4	-0.046015893715104284
11411	5	-0.7105752778261518
11411	6	-0.605271780444923
11411	7	-1.0969695173206375
11411	8	1.1216357435231075
11411	9	-0.008698627199613895
11411	10-11	0.14192539237836144
11411	12-13	1.0304788184633935
11411	14-15	1.6373223833001163
11411	16-17	-0.444519104498049
11411	18-19	1.6394239915100073
11411	20-21	2.2651420765575594
11411	22-23	0.9796783842410406
11411	24-25	2.2570313792371337
11411	26-27	1.0540848307056336
11411	28-29	1.8923196747127875
11411	30-31	2.3431732431064773
11411	32-33	1.4844624924337673
11411	34-35	2.2932246739119577
11411	36-37	2.5854960976612418
11411	38-39	2.8144112755358215
11411	40-41	2.0069504851822977
11411	42-43	1.225730257514229
11411	44-45	1.867283964021297
11411	46-47	1.1218286200109198
11411	48-49	3.055840172994678
11411	50-51	3.035925092469693
11411	52-53	1.6314654350289501
11411	54-55	0.2806822305218226
11411	56-57	2.539878671557833
11411	58-59	4.385351520867445
11411	60-61	1.4571067617265
11411	62-63	0.9399802999377158
11411	64-65	1.4957430011647688
11411	66-67	-1.0280166721179782
11411	68-69	3.064261994519814
11411	70-71	4.329200745196527
11411	72-73	1.9039329196571728
11411	74-75	3.108533154569436
11411	76	3.1145355798005063
13411	1	0.570250586358231
13411	2	-0.02892341500228568
13411	3	-0.640687256469409
13411	4	0.027959150848175796
13411	5	-0.5020191280935329
13411	6	-1.2059847929226422
13411	7	-0.37058805564505803
13411	8	-1.5263143456034527
13411	9	-0.2582529944010403
13411	10-11	-1.4873081192080875
13411	12-13	-1.048843819682773
13411	14-15	-0.6006455311383903
13411	16-17	0.08801208979785713
13411	18-19	-0.9127150459231501
13411	20-21	-0.6987616667579459
13411	22-23	0.009535781745498184
13411	24-25	-0.45018787209976807
13411	26-27	-0.317127290506491
13411	28-29	0.7301093360318589
13411	30-31	0.7598399097731487
13411	32-33	0.05932880259419804
13411	34-35	-1.090465165660234
13411	36-37	-2.1591563087558754
13411	38-39	-0.3852322182253154
13411	40-41	-0.8281653793453287
13411	42-43	-1.1628615428422755
13411	44-45	-2.0484914370482237
13411	46-47	-1.6593656758892585
13411	48-49	-1.0047658876113843
13411	50-51	-1.9801176882789697
13411	52-53	-2.643935634489768
13411	54-55	0.023997738543211966
13411	56-57	-2.3112799737184524
13411	58-59	-1.5772153240523394
13411	60-61	-3.599488603692393
13411	62-63	-1.384886010222715
13411	64-65	-1.153543986357512
13411	66-67	-1.006180664987852
13411	68-69	0.2202334740207057
13411	70-71	-0.6917439963364487
13411	72-73	1.4030416540600257
13411	74-75	2.1732537796615787
13411	76	1.4012739310549875
21411	1	-0.14239309180268833
21411	2	1.2286523425734721
21411	3	0.5792709462474157
21411	4	0.7024628081104538
21411	5	1.02776143616979
21411	6	0.8389472864921927
21411	7	1.6565801784197305
21411	8	1.2108852364237137
21411	9	0.7610782490681345
21411	10-11	0.09628644714509704
21411	12-13	0.32053967039036735
21411	14-15	-1.139046785056884
21411	16-17	0.3095376906338032
21411	18-19	1.2479432612868848
21411	20-21	-0.27086198023756936
21411	22-23	0.2702463355594986
21411	24-25	0.3625080526651274
21411	26-27	-0.04936344515643043
21411	28-29	-0.6512908729545543
21411	30-31	-0.6740681362038679
21411	32-33	-0.6585395359637971
21411	34-35	0.33429972462189994
21411	36-37	0.31419792321904794
21411	38-39	0.014715535170712002
21411	40-41	0.5054291870078593
21411	42-43	-2.0800506755486516
21411	44-45	-0.21385193861561902
21411	46-47	1.2460679709237006
21411	48-49	0.8661850005808844
21411	50-51	-0.236387280755455
21411	52-53	0.9697007291465951
21411	54-55	0.699546327884903
21411	56-57	0.4557001725720369
21411	58-59	0.5962159029179048
21411	60-61	0.7063327679184503
21411	62-63	0.27021765803885245
21411	64-65	-0.01199693691572179
21411	66-67	0.9575354434650976
21411	68-69	1.4172031709904012
21411	70-71	1.00252007292762
21411	72-73	0.8697083207266942
21411	74-75	0.44199381155950235
21411	76	-0.13692640850358728
23411	1	-0.4884348596511572
23411	2	0.6511875538410763
23411	3	0.6079256329885503
23411	4	0.37900481879525216
23411	5	-0.5225056837913549
23411	6	0.4576942510380917
23411	7	0.5873718248500381
23411	8	0.2856788255446503
23411	9	0.8744828143911079
23411	10-11	-0.05111520414193649
23411	12-13	0.7678441871460748
23411	14-15	0.49026355977070324
23411	16-17	-0.15537245992472037
23411	18-19	-0.05900185770291699
23411	20-21	0.8732613806172154
23411	22-23	0.8711448299742699
23411	24-25	1.0213230113829468
23411	26-27	1.5470425771845129
23411	28-29	0.7382185976622466
23411	30-31	0.6037366233881727
23411	32-33	2.0420432712241627
23411	34-35	0.7754119150055772
23411	36-37	2.268180439003416
23411	38-39	1.2336324851464333
23411	40-41	2.0939430286785736
23411	42-43	2.4577103735042876
23411	44-45	2.0984355795971084
23411	46-47	2.3413812297872276
23411	48-49	1.830488060318622
23411	50-51	1.4949143219643055
23411	52-53	1.677447208019835
23411	54-55	1.1385944094779141
23411	56-57	1.7055787544078775
23411	58-59	0.4669588116710912
23411	60-61	2.124049595197917
23411	62-63	1.7415544507247915
23411	64-65	2.651086828836686
23411	66-67	3.0084373461090337
23411	68-69	2.304527114652373
23411	70-71	0.7422082017002545
23411	72-73	2.377006656194041
23411	74-75	1.5586224370674948
23411	76	3.4521424174073445
11412	1	0.10178211788976199
11412	2	-0.1091854952643665
11412	3	-0.5293031450852972
11412	4	-0.5953004724114486
11412	5	-0.9172525433269456
11412	6	-1.8087693957072482
11412	7	-1.094584779641778
11412	8	0.09858327929417499
11412	9	-2.8409720771201243
11412	10-11	-0.07985521175518073
11412	12-13	-1.0883606091995617
11412	14-15	0.5510743705815102
11412	16-17	-0.4707512189654537
11412	18-19	-1.376474259682361
11412	20-21	0.07396560596932744
11412	22-23	-0.7083184361087191
11412	24-25	-0.4790576509695441
11412	26-27	-1.4324016557808505
11412	28-29	-1.0587932028706817
11412	30-31	0.4715516214848563
11412	32-33	-0.8509906077252118
11412	34-35	-0.7004160256110907
11412	36-37	-0.36124476084432544
11412	38-39	0.30805197505887705
11412	40-41	-0.3599016610816115
11412	42-43	-0.5656051955859276
11412	44-45	-1.8783440169008045
11412	46-47	1.0566457901222073
11412	48-49	-0.7752409080864027
11412	50-51	-2.3535820617433423
11412	52-53	-2.1113803519344856
11412	54-55	-1.1271079125624404
11412	56-57	-2.1016157640542446
11412	58-59	-2.6607534075904233
11412	60-61	-2.2495705037742937
11412	62-63	-1.6299720053087086
11412	64-65	-1.7467053295188535
11412	66-67	-1.01211842092561
11412	68-69	-0.5017157479285181
11412	70-71	-2.085787167776882
11412	72-73	-1.4296690142670805
11412	74-75	-0.8755857568621899
11412	76	-3.7882421979772722
13412	1	-0.24720973110208533
13412	2	0.1175412314623614
13412	3	0.14358402780187518
13412	4	0.3620139849030082
13412	5	-0.2754679015423065
13412	6	-0.6388852258230742
13412	7	0.607045421988424
13412	8	0.7658934466043377
13412	9	-0.2972140333620814
13412	10-11	0.43044079854082185
13412	12-13	-0.034413805252761165
13412	14-15	0.4347080042151461
13412	16-17	-0.418842167056404
13412	18-19	-0.4455433287514339
13412	20-21	-0.9300026479989256
13412	22-23	-0.8191077968980807
13412	24-25	0.3690761971643042
13412	26-27	0.31598959261039283
13412	28-29	-0.556326450403926
13412	30-31	-1.2044458045125737
13412	32-33	0.8868901301555248
13412	34-35	1.022449697254629
13412	36-37	0.20267630307673912
13412	38-39	-0.8830677160608111
13412	40-41	-1.45009900127895
13412	42-43	-1.1967720767528114
13412	44-45	0.34580870725191915
13412	46-47	0.23060546408188287
13412	48-49	0.08758620474070966
13412	50-51	0.3827971746358969
13412	52-53	-1.613632604186737
13412	54-55	-1.478888264342789
13412	56-57	0.09636793392945364
13412	58-59	-1.2467679936050118
13412	60-61	-0.5442937984975913
13412	62-63	-0.676733052069757
13412	64-65	0.6007850179714964
13412	66-67	-0.863684272491458
13412	68-69	-3.2891460353588045
13412	70-71	0.905297850705395
13412	72-73	0.5127097637281359
13412	74-75	-1.9076405465494268
13412	76	-1.3854644201994937
12410	1	0.4035839196915667
12410	2	0.6831977971189254
12410	3	-0.8225054382875889
12410	4	0.11886824175726574
12410	5	-0.18383730991171277
12410	6	0.7485606616228111
12410	7	0.03850285344585558
12410	8	-0.586920406209515
12410	9	-0.8491620853101338
12410	10-11	-0.2221566040565719
12410	12-13	-0.3518741227130775
12410	14-15	-0.11579704628990584
12410	16-17	-0.44229094050517404
12410	18-19	0.2615273783192791
12410	20-21	-0.21391318190946151
12410	22-23	-0.982888460678744
12410	24-25	-0.6774605993724947
12410	26-27	0.44802422464502456
12410	28-29	-0.47443611851359435
12410	30-31	0.5098399097731416
12410	32-33	1.0138742571396513
12410	34-35	-0.22682880202386713
12410	36-37	-0.21976236936193416
12410	38-39	0.09961626662316903
12410	40-41	0.2627437115637612
12410	42-43	-0.22346760344833783
12410	44-45	0.17120553264874872
12410	46-47	0.8860888695652882
12410	48-49	0.5028098699643735
12410	50-51	0.4213974632361861
12410	52-53	1.4242461836920484
12410	54-55	1.4255128900583642
12410	56-57	-0.8112799737184524
12410	58-59	-0.6605486573856751
12410	60-61	-0.06918557338936182
12410	62-63	-0.9985223738590783
12410	64-65	0.05857722576369895
12410	66-67	-0.9531503619575474
12410	68-69	-0.8858271320399034
12410	70-71	0.6946196400271845
12410	72-73	1.0469810479994237
12410	74-75	0.6568031957237288
12410	76	2.105988571253498
12411	1	0.23691725302489886
12411	2	-1.3622567483356178
12411	3	0.6623430465608955
12411	4	-0.03264690975788653
12411	5	1.4070717809973772
12411	6	1.354621267683413
12411	7	0.4324422473852465
12411	8	0.913079593790485
12411	9	0.8629591268110772
12411	10-11	1.0429949110949366
12411	12-13	0.19358042274146925
12411	14-15	0.9220817415888902
12411	16-17	0.6183151201008883
12411	18-19	-0.02635140955951698
12411	20-21	-0.42603439403067256
12411	22-23	-0.21016118795147065
12411	24-25	-0.45018787209976807
12411	26-27	-0.6050060783852729
12411	28-29	0.017988123910647857
12411	30-31	-0.2477358478026126
12411	32-33	-0.14521665195125522
12411	34-35	0.045898470703402694
12411	36-37	-0.8409744905740553
12411	38-39	0.5162829332898369
12411	40-41	1.088501287321339
12411	42-43	1.5947142147334823
12411	44-45	-0.11667325523004024
12411	46-47	1.355785839262257
12411	48-49	0.10129471844922477
12411	50-51	0.565336857175577
12411	52-53	1.6742461836920484
12411	54-55	1.433088647634122
12411	56-57	0.4917503293118486
12411	58-59	1.1500574032203872
12411	60-61	0.915662911459119
12411	62-63	2.865113989777285
12411	64-65	0.013122680309159307
12411	66-67	-1.4758776346848208
12411	68-69	1.060060313847547
12411	70-71	0.6751391205466675
12411	72-73	-1.5428457918274177
12411	74-75	-0.19328757372187866
12411	76	0.6367578020227285
22411	1	0.3369172530249003
22411	2	-1.0622567483356171
22411	3	-0.4194751352572865
22411	4	-0.7144650915760664
22411	5	0.26161723554283256
22411	6	0.6909849040470561
22411	7	-0.6766486617056628
22411	8	-0.3596476789367884
22411	9	0.590231854083811
22411	10-11	-0.4751869070868793
22411	12-13	-0.27914684998580697
22411	14-15	-0.5279182584111126
22411	16-17	0.6410423928281546
22411	18-19	0.10092131771320823
22411	20-21	-0.6396707576670408
22411	22-23	0.6534751756848891
22411	24-25	-0.37291514482704
22411	26-27	-0.7095515329307318
22411	28-29	0.39071539663791555
22411	30-31	0.3886277885610241
22411	32-33	-0.1224893792239854
22411	34-35	-1.504101529296598
22411	36-37	-0.2909744905740581
22411	38-39	0.9026465696534736
22411	40-41	-0.9705896217695731
22411	42-43	-0.050740330721065874
22411	44-45	-0.20758234613913373
22411	46-47	-0.8305777971013804
22411	48-49	-1.45325073609623
22411	50-51	-0.7164813246426043
22411	52-53	-0.27120836176249696
22411	54-55	-0.4669113523658801
22411	56-57	0.10993214749366942
22411	58-59	0.9591483123112958
22411	60-61	0.04748109327730177
22411	62-63	-1.4257951011318042
22411	64-65	-1.1005136833272076
22411	66-67	1.2104860016788166
22411	68-69	-1.8373422835550528
22411	70-71	-1.9841682387606916
22411	72-73	-1.0302916792733043
22411	74-75	-2.108606531493667
22411	76	-2.481424016159089
12412	1	-0.5130827469751011
12412	2	-0.3675015035803746
12412	3	-0.3603842261663779
12412	4	-0.4487308258417997
12412	5	0.15707178099737718
12412	6	0.14133455439669973
12412	7	-0.8560192910762936
12412	8	-1.2460113153004215
12412	9	1.1304416442935974
12412	10-11	-0.02606103296100315
12412	12-13	0.7250489542100027
12412	14-15	-0.6635825940754501
12412	16-17	-0.19112543933967174
12412	18-19	-0.5822954655035701
12412	20-21	0.5258886828924076
12412	22-23	0.436691958901676
12412	24-25	-0.010502557414447722
12412	26-27	0.7367771383979402
12412	28-29	0.024107005029527784
12412	30-31	-0.5702882953550592
12412	32-33	-1.9572795890141954
12412	34-35	-0.516164466359534
12412	36-37	-0.7159744905740553
12412	38-39	-1.7896611226542198
12412	40-41	-0.8188413700213211
12412	42-43	1.4627212077404756
12412	44-45	0.8571029685461795
12412	46-47	-3.069913461437043
12412	48-49	-1.0114675193130154
12412	50-51	-0.6365862197474996
12412	52-53	0.7773930368389017
12412	54-55	0.2031935427390188
12412	56-57	-0.21803988047836143
12412	58-59	-0.5518656737026895
12412	60-61	0.4882153590115692
12412	62-63	0.7182608429241384
12412	64-65	0.19144435863083586
12412	66-67	0.29947201566482917
12412	68-69	0.051818555605787964
12412	70-71	-1.3044719546526444
12412	72-73	-1.9588121783820398
12412	74-75	0.6152838548495474
12412	76	0.8034244686893928
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	6.0
21	31.0
22	62.0
23	108.0
24	131.0
25	173.0
26	200.0
27	336.0
28	463.0
29	569.0
30	831.0
31	1035.0
32	984.0
33	717.0
34	245.0
35	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.527970255196887	20.584755788406287	32.24607064390739	18.641203312489438
2	35.82896738211932	20.48335305053236	24.269055264492142	19.418624302856177
3	35.0684468480649	17.458171370627007	23.00152104106811	24.471860740239986
4	41.18641203312489	19.89183707960115	20.855163089403415	18.066587797870543
5	33.53050532364374	24.404258914990702	20.077742099036673	21.987493662328884
6	32.11086699340882	21.48047997295927	18.74260605036336	27.66604698326855
7	28.037857022139594	19.689031603853305	22.748014196383302	29.525097177623792
8	25.09717762379584	15.041406117965185	29.896907216494846	29.964509041744126
9	23.525435186750045	17.50887273956397	25.976001352036505	32.98969072164948
10-11	27.175933750211257	25.976001352036505	24.023998647963495	22.824066249788743
12-13	23.358404462097525	33.04318431505113	28.243049099974648	15.3553621228767
14-15	17.97363528815278	33.555856008112215	28.64627344938313	19.82423525435187
16-17	22.967720128443467	36.21767787730269	23.550785871218523	17.263816123035323
18-19	25.511238803447693	30.277167483522057	23.212776744972114	20.998816968058136
20-21	29.863106303870207	25.012675342234242	29.618049687341557	15.506168666553997
22-23	31.874260605036337	24.96197397329728	24.497211424708468	18.66655399695792
24-25	27.513942876457666	32.20381950312658	20.41575122528308	19.866486395132668
26-27	18.522900118303195	31.215142808855838	19.96788913300659	30.294067939834374
28-29	20.576305560250127	26.744972114247084	20.365049856346122	32.31367246915667
30-31	21.07486902146358	25.553489944228495	27.091431468649652	26.280209565658275
32-33	23.79584248774717	34.11357106641879	26.449214128781477	15.64137231705256
34-35	18.429947608585433	40.98360655737705	29.432144667906034	11.154301166131486
36-37	14.323136724691565	34.07977015379415	40.98360655737705	10.613486564137231
38-39	15.541282853038114	43.62376404969154	30.17831488210935	10.65663821516099
40-41	21.92834206523576	40.34138921750887	20.407300997126924	17.322967720128442
42-43	18.81020787561264	39.53016731451749	24.725367584924793	16.934257224945075
44-45	13.664018928511071	41.17796180496873	32.651681595403076	12.506337671117121
46-47	14.855501098529661	40.5695453777252	34.11357106641879	10.461382457326348
48-49	21.733986817644077	37.54436369781985	28.64627344938313	12.07537603515295
50-51	18.886259929018085	40.01183031941862	22.65506168666554	18.446848064897754
52-53	23.964847050870375	38.69359472705763	16.630049011323305	20.71150921074869
54-55	32.64599002788811	35.40099721118905	15.05112820079439	16.901884560128455
56-57	33.53900769165751	40.36852337080551	15.391767390753106	10.700701546783872
58-59	30.273057739453886	45.90413390819173	14.811057570377884	9.011750781976497
60-61	20.879492600422832	55.31501057082452	15.272727272727273	8.53276955602537
62-63	16.218274111675125	61.20135363790185	14.255499153976311	8.3248730964467
64-65	15.474576271186441	62.0593220338983	13.94915254237288	8.516949152542374
66-67	16.150123121338204	61.993716566188326	13.407489173813364	8.448671138660101
68-69	17.04951505870342	62.10651693040668	13.033860813340139	7.810107197549771
70-71	18.763326226012794	61.253731343283576	12.238805970149254	7.744136460554371
72-73	20.049653283109322	60.440030819279166	12.088006163855834	7.422309733755672
74-75	23.289545703338806	56.81444991789819	12.059843094325853	7.8361612844371455
76	51.72120696982575	0.0	27.369315767105824	20.909477263068425
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	2.5
7	5.5
8	8.0
9	13.0
10	20.0
11	23.0
12	25.0
13	31.0
14	37.0
15	40.0
16	48.5
17	61.5
18	65.0
19	69.5
20	77.5
21	82.5
22	98.5
23	109.5
24	120.0
25	135.5
26	141.0
27	138.0
28	144.5
29	155.0
30	159.0
31	169.0
32	177.5
33	174.5
34	166.0
35	172.0
36	187.5
37	186.5
38	173.5
39	193.5
40	230.0
41	422.0
42	691.0
43	640.5
44	466.0
45	324.0
46	185.0
47	120.0
48	84.0
49	78.5
50	72.0
51	67.5
52	67.0
53	65.0
54	62.0
55	64.5
56	60.5
57	60.0
58	68.0
59	67.0
60	56.0
61	44.5
62	44.0
63	43.0
64	37.5
65	28.5
66	22.0
67	21.5
68	19.0
69	15.0
70	16.5
71	17.0
72	13.0
73	11.5
74	13.5
75	14.0
76	14.0
77	10.5
78	4.0
79	1.5
80	1.5
81	1.5
82	0.5
83	0.0
84	0.0
85	0.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	1.5
98	1.5
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.008450228156160216
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.008450228156160216
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
54	1.0
55	0.0
56	1.0
57	0.0
58	1.0
59	1.0
60	1.0
61	1.0
62	2.0
63	7.0
64	4.0
65	5.0
66	9.0
67	6.0
68	2.0
69	9.0
70	9.0
71	8.0
72	19.0
73	90.0
74	520.0
75	2868.0
76	2353.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.023322629711
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.56188605108055	83.06574277505493
2	1.6699410609037328	2.8730775730944735
3	0.4911591355599214	1.2675342234240323
4	0.3339882121807466	1.1492310292377894
5	0.275049115913556	1.1830319418624302
6	0.07858546168958742	0.4056109514956904
7	0.09823182711198428	0.5915159709312151
8	0.019646365422396856	0.13520365049856345
9	0.11787819253438114	0.9126246408653035
>10	0.3339882121807466	6.422173398681765
>50	0.0	0.0
>100	0.019646365422396856	1.9942538448538112
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	118	1.9942538448538112	No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAAA	40	0.6760182524928173	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	36	0.6084164272435356	No Hit
AGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	33	0.5577150583065743	No Hit
TCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	30	0.507013689369613	No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	29	0.4901132330572926	No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	28	0.4732127767449721	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAAAAAAAAAAAAAA	27	0.45631232043265174	No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	25	0.4225114078080108	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC	22	0.3718100388710495	No Hit
GCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	18	0.3042082136217678	No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCA	18	0.3042082136217678	No Hit
AAGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	16	0.2704073009971269	No Hit
TAGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	14	0.23660638837248604	No Hit
TACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAA	12	0.2028054757478452	No Hit
CGTGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	11	0.18590501943552476	No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAAAAAAAAAAAAAAA	11	0.18590501943552476	No Hit
GAGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	10	0.16900456312320433	No Hit
GGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGC	9	0.1521041068108839	No Hit
AACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAA	9	0.1521041068108839	No Hit
GTCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAA	9	0.1521041068108839	No Hit
ATCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAA	9	0.1521041068108839	No Hit
CACGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	9	0.1521041068108839	No Hit
AGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	9	0.1521041068108839	No Hit
GTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGCA	8	0.13520365049856345	No Hit
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	7	0.11830319418624302	No Hit
GTAGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAA	7	0.11830319418624302	No Hit
CGCGTACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	7	0.11830319418624302	No Hit
AGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAAAAAAAAAAAAAA	7	0.11830319418624302	No Hit
TTCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAA	7	0.11830319418624302	No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	6	0.1014027378739226	No Hit
AGGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	6	0.1014027378739226	No Hit
GGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	6	0.1014027378739226	No Hit
GTGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	6	0.1014027378739226	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10	0.0	0.0	0.0
11	0.0	0.0	0.0
12	0.0	0.0	0.0
13	0.0	0.0	0.0
14	0.0	0.0	0.0
15	0.0	0.0	0.0
16	0.0	0.0	0.0
17	0.0	0.0	0.0
18	0.0	0.0	0.0
19	0.0	0.0	0.0
20	0.0	0.0	0.0
21	0.0	0.0	0.0
22	0.0	0.0	0.0
23	0.01690045631232043	0.0	0.0
24	0.01690045631232043	0.0	0.0
25	0.01690045631232043	0.0	0.0
26	0.0507013689369613	0.0	0.0
27	0.08450228156160217	0.0	0.0
28	0.11830319418624304	0.0	0.0
29	0.11830319418624304	0.0	0.0
30	0.13520365049856345	0.0	0.0
31	0.16900456312320433	0.0	0.0
32	0.2028054757478452	0.0	0.0
33	0.21970593206016562	0.0	0.0
34	0.23660638837248607	0.0	0.0
35	0.2535068446848065	0.0	0.0
36	0.2535068446848065	0.0	0.0
37	0.2535068446848065	0.0	0.0
38	0.28730775730944735	0.0	0.0
39	0.3211086699340882	0.0	0.0
40	0.3718100388710495	0.0	0.0
41	0.3718100388710495	0.0	0.0
42	0.38871049518337	0.0	0.0
43	0.4056109514956904	0.0	0.0
44	0.4056109514956904	0.0	0.0
45	0.4056109514956904	0.0	0.0
46	0.4056109514956904	0.0	0.0
47	0.43941186412033123	0.0	0.0
48	0.43941186412033123	0.0	0.0
49	0.4563123204326517	0.0	0.0
50	0.4563123204326517	0.0	0.0
51	0.5239141456819334	0.0	0.0
52	0.5577150583065743	0.0	0.0
53	0.5915159709312151	0.0	0.0
54	0.6084164272435356	0.0	0.0
55	0.625316883555856	0.0	0.0
56	0.6591177961804969	0.0	0.0
57	0.6929187088051377	0.0	0.0
58	0.7267196214297786	0.0	0.0
59	0.7605205340544194	0.0	0.0
60	0.8112219029913807	0.0	0.0
61	0.9295250971776238	0.0	0.0
62	0.9633260098022647	0.0	0.0
63	0.9802264661145851	0.0	0.0
64	0.9802264661145851	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAAAAGG	45	1.5019941E-7	107.85714	70
TACGCGA	20	0.006962639	51.82627	2
ACGCGAC	20	0.006962639	51.82627	3
ACCTCAG	150	0.0	34.55085	5
GACCTCA	150	0.0	34.55085	4
CGCGACC	90	3.5324774E-9	34.550846	1
GCGACCT	130	0.0	34.550846	2
ATCAGAC	185	0.0	33.61704	12
GACGTGG	185	0.0	33.61704	16
TCAGATC	185	0.0	33.61704	8
TCAGACG	185	0.0	33.61704	13
CAGACGT	185	0.0	33.61704	14
GATCAGA	185	0.0	33.61704	11
CAGATCA	185	0.0	33.61704	9
AGATCAG	185	0.0	33.61704	10
CGACCTC	145	0.0	33.35944	3
GCGACCC	190	0.0	32.732384	22
TAAGCAT	190	0.0	32.732384	37
AGACGTG	190	0.0	32.732384	15
TTAAGCA	190	0.0	32.732384	36
>>END_MODULE
