FastQCFastQC Report
Wed 15 Feb 2017
OU_LexBC006_S46_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOU_LexBC006_S46_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34120
Sequences flagged as poor quality0
Sequence length15-76
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA4011.175263774912075No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAAA1510.4425556858147714No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA1080.31652989449003516No Hit
AGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA1040.30480656506447834No Hit
TCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA980.28722157092614303No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAAAAAAAAAAAAAA910.26670574443141853No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA850.24912075029308325No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA780.22860492379835873No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA740.21688159437280186No Hit
AAGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA550.1611957796014068No Hit
GCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA550.1611957796014068No Hit
GGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGC490.14361078546307152No Hit
ATGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA490.14361078546307152No Hit
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA470.13774912075029308No Hit
GTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGCA440.12895662368112545No Hit
AACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAA410.1201641266119578No Hit
GGGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTG400.11723329425556857No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCA370.10844079718640093No Hit
ATCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAA360.10550996483001172No Hit
GGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA360.10550996483001172No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGG100.004441818168.953470
TACGCGA150.00232026669.11731
GAGTGGA200.00724942851.8379757
AGTGGAA200.00724942851.8379758
AAAAAGG1008.872721E-750.6860270
AAGCGAC306.173373E-446.07822
TGCGACC450.00452297130.71883
TCGCGAC658.4015203E-426.583583
AGCGACC851.387541E-424.394343
CGCGACC2103.6379788E-1223.03911
TCAGATC5700.020.0076398
CCTCAGA5050.019.8455646
GCGACCT3850.019.74782
TCAGACG5850.019.49462313
GATCAGA5850.019.49462311
CAGATCA5850.019.4946239
ACGTGGC5850.019.49462317
ACCTCAG4450.019.4149725
GACCTCA4450.019.4149724
CGTGGCG5900.019.32941418
Produced by FastQC (version 0.11.2)