##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	OU_LexBC005_S45_L001_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	33986
Sequences flagged as poor quality	0
Sequence length	21-76
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.198876007767904	32.0	32.0	32.0	32.0	32.0
2	31.29114929676926	32.0	32.0	32.0	32.0	32.0
3	31.432560466074264	32.0	32.0	32.0	32.0	32.0
4	31.271023362561056	32.0	32.0	32.0	32.0	32.0
5	31.2105867121756	32.0	32.0	32.0	32.0	32.0
6	34.7206496792797	36.0	36.0	36.0	32.0	36.0
7	34.602071441181664	36.0	36.0	36.0	32.0	36.0
8	34.55393397281234	36.0	36.0	36.0	32.0	36.0
9	34.53878067439534	36.0	36.0	36.0	32.0	36.0
10-11	34.44580121226387	36.0	36.0	36.0	32.0	36.0
12-13	34.10336609192021	36.0	36.0	36.0	32.0	36.0
14-15	33.759239098452305	36.0	36.0	36.0	29.5	36.0
16-17	33.763608544694875	36.0	36.0	36.0	27.0	36.0
18-19	33.680824457129404	36.0	36.0	36.0	29.5	36.0
20-21	33.24661625375154	36.0	32.0	36.0	27.0	36.0
22-23	33.128030052723496	36.0	32.0	36.0	27.0	36.0
24-25	33.042166902071564	36.0	32.0	36.0	27.0	36.0
26-27	32.72797419552991	36.0	32.0	36.0	24.0	36.0
28-29	32.23181390147725	36.0	32.0	36.0	17.5	36.0
30-31	31.39939090821183	34.0	29.5	36.0	14.0	36.0
32-33	31.08912173298799	34.0	29.5	36.0	14.0	36.0
34-35	31.720464445490933	36.0	32.0	36.0	14.0	36.0
36-37	30.561542137165617	34.0	29.5	36.0	14.0	36.0
38-39	28.079112024508238	29.5	24.0	34.0	14.0	36.0
40-41	28.62314190680835	32.0	27.0	34.0	14.0	36.0
42-43	29.42310069761281	32.0	27.0	36.0	14.0	36.0
44-45	28.996570806228476	32.0	27.0	36.0	14.0	36.0
46-47	29.011832926147235	32.0	27.0	36.0	14.0	36.0
48-49	29.854413799193477	32.0	27.0	36.0	14.0	36.0
50-51	29.936800894854585	32.0	27.0	36.0	14.0	36.0
52-53	30.237362175956843	34.0	27.0	36.0	14.0	36.0
54-55	31.19735821614589	36.0	32.0	36.0	14.0	36.0
56-57	31.307114671064255	36.0	32.0	36.0	14.0	36.0
58-59	30.570607589771242	36.0	27.0	36.0	14.0	36.0
60-61	29.742803082398474	32.0	27.0	36.0	14.0	36.0
62-63	29.596438720771786	34.0	27.0	36.0	14.0	36.0
64-65	29.9344560345861	34.0	27.0	36.0	14.0	36.0
66-67	29.202423073210703	32.0	27.0	36.0	14.0	36.0
68-69	28.363338225554642	32.0	24.0	36.0	14.0	36.0
70-71	27.40217988280189	32.0	21.0	36.0	14.0	36.0
72-73	25.71968702708903	27.0	14.0	32.0	14.0	36.0
74-75	25.060035264122455	27.0	14.0	32.0	14.0	36.0
76	24.731126551643374	27.0	14.0	32.0	14.0	36.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
12301	1	1.6681632094317997
12301	2	0.6842657223383917
12301	3	0.025113536473739373
12301	4	-0.09970684487474912
12301	5	-0.2323697403952174
12301	6	0.3282821692013229
12301	7	0.7767475878653585
12301	8	1.4846914759511805
12301	9	1.1432161924080901
12301	10-11	0.9311117476154607
12301	12-13	1.0763720687694374
12301	14-15	1.3142864147821456
12301	16-17	1.516618548129955
12301	18-19	2.2524036781202916
12301	20-21	0.0970751319286478
12301	22-23	0.32570776434549487
12301	24-25	-0.18707013320799604
12301	26-27	1.467767804841106
12301	28-29	1.6192699112473221
12301	30-31	-0.12103770495715338
12301	32-33	0.5626500993819228
12301	34-35	0.30715474582157043
12301	36-37	2.5622997483862413
12301	38-39	-0.3723223381394156
12301	40-41	-0.07036466026087851
12301	42-43	-0.6167910708069222
12301	44-45	-1.3525069744251361
12301	46-47	-2.3528776714749853
12301	48-49	0.9422321627877643
12301	50-51	1.6392910418925588
12301	52-53	0.8801811761968388
12301	54-55	1.0915902524404615
12301	56-57	0.510494446335727
12301	58-59	0.8123214564587862
12301	60-61	-0.09354707955936092
12301	62-63	-1.1686728847466483
12301	64-65	1.4339293620379259
12301	66-67	0.6057916593604098
12301	68-69	1.4555100045206295
12301	70-71	-0.3149887033174963
12301	72-73	0.06553173078108898
12301	74-75	2.238387508166234
12301	76	6.5211447036156756
13201	1	2.3844058708799416
13201	2	0.9273185599117753
13201	3	-0.16627589601158377
13201	4	0.6224066580606724
13201	5	0.7108787918944124
13201	6	1.07427042751835
13201	7	1.5998395643819947
13201	8	1.7105231784952153
13201	9	1.4465430025842139
13201	10-11	1.4299375793180076
13201	12-13	1.5941802879475304
13201	14-15	2.4336601916901657
13201	16-17	2.5240549473471745
13201	18-19	1.3338126800772336
13201	20-21	0.5463902004217971
13201	22-23	1.480306590177193
13201	24-25	-0.24969244240564592
13201	26-27	0.8695290572872914
13201	28-29	3.1828706940261924
13201	30-31	-0.47407097305891455
13201	32-33	1.6158790621999266
13201	34-35	0.7621449610857596
13201	36-37	-0.7557041655080887
13201	38-39	-1.35138300350145
13201	40-41	-0.5987403941160636
13201	42-43	-0.6283370590652417
13201	44-45	-0.7544639215875613
13201	46-47	-1.5331125051344792
13201	48-49	-0.7399596180341526
13201	50-51	0.087431942088255
13201	52-53	-0.546824694644652
13201	54-55	-0.5452003542131578
13201	56-57	0.7288897496625388
13201	58-59	-0.32564331849229333
13201	60-61	-0.4387525590114123
13201	62-63	-2.8529866102368437
13201	64-65	-2.8497430833181703
13201	66-67	-1.8661627339143934
13201	68-69	0.30714749746246994
13201	70-71	-0.5038932770046785
13201	72-73	1.0288701143088836
13201	74-75	2.032168962945562
13201	76	4.337364941710916
12307	1	2.3844058708799416
12307	2	1.4416042741974913
12307	3	0.34800981827413224
12307	4	0.6224066580606724
12307	5	-8.289121208105588
12307	6	1.7314132846612083
12307	7	1.7141252786677086
12307	8	0.8819517499237861
12307	9	2.446543002584214
12307	10-11	1.2442232936037243
12307	12-13	1.9227517165189525
12307	14-15	3.290803048833027
12307	16-17	-1.8473736240813992
12307	18-19	4.090955537220093
12307	20-21	2.0892473432789416
12307	22-23	2.5517351616057624
12307	24-25	3.250307557594354
12307	26-27	-7.787613799855567
12307	28-29	-6.174272163116669
12307	30-31	2.6045004555125146
12307	32-33	-1.5126923663714997
12307	34-35	3.7621449610857596
12307	36-37	-9.112847022650943
12307	38-39	-5.158525860644307
12307	40-41	5.615545320169652
12307	42-43	3.9145200837919027
12307	44-45	-4.568749635873278
12307	46-47	-2.2902553622773354
12307	48-49	-4.854245332319866
12307	50-51	1.8731462278025397
12307	52-53	-3.346824694644649
12307	54-55	-1.1452003542131592
12307	56-57	-0.29968167890888964
12307	58-59	0.888642395793422
12307	60-61	-2.003038273297129
12307	62-63	0.8970133897631563
12307	64-65	2.3859712023961173
12307	66-67	-5.859019876771537
12307	68-69	3.3428617831767546
12307	70-71	1.1889638658524646
12307	72-73	-1.2497013142625448
12307	74-75	-3.8537336739712735
12307	76	1.7659363702823434
12309	1	2.3844058708799416
12309	2	1.4416042741974913
12309	3	0.34800981827413224
12309	4	0.6224066580606724
12309	5	0.7108787918944124
12309	6	1.7314132846612083
12309	7	1.7141252786677086
12309	8	2.881951749923786
12309	9	2.446543002584214
12309	10-11	2.2442232936037243
12309	12-13	2.9227517165189525
12309	14-15	1.2908030488330269
12309	16-17	3.652626375918601
12309	18-19	4.090955537220093
12309	20-21	3.0892473432789416
12309	22-23	3.5517351616057624
12309	24-25	3.250307557594354
12309	26-27	4.212386200144433
12309	28-29	0.3257278368833312
12309	30-31	4.854500455512515
12309	32-33	-5.0126923663715
12309	34-35	2.7621449610857596
12309	36-37	2.887152977349057
12309	38-39	-0.908525860644307
12309	40-41	-1.8844546798303483
12309	42-43	-3.5854799162080973
12309	44-45	2.431250364126722
12309	46-47	4.209744637722665
12309	48-49	-3.8542453323198664
12309	50-51	5.37314622780254
12309	52-53	6.653175305355351
12309	54-55	5.354799645786841
12309	56-57	1.2003183210911104
12309	58-59	6.388642395793422
12309	60-61	6.746961726702871
12309	62-63	5.147013389763156
12309	64-65	5.135971202396117
12309	66-67	7.390980123228463
12309	68-69	8.092861783176755
12309	70-71	-2.0610361341475354
12309	72-73	5.500298685737455
12309	74-75	-1.8537336739712735
12309	76	13.265936370282343
12310	1	2.3844058708799416
12310	2	1.4416042741974913
12310	3	0.34800981827413224
12310	4	0.6224066580606724
12310	5	0.7108787918944124
12310	6	1.7314132846612083
12310	7	1.7141252786677086
12310	8	2.881951749923786
12310	9	2.446543002584214
12310	10-11	2.2442232936037243
12310	12-13	0.9227517165189525
12310	14-15	3.290803048833027
12310	16-17	3.652626375918601
12310	18-19	4.090955537220093
12310	20-21	3.0892473432789416
12310	22-23	3.5517351616057624
12310	24-25	3.250307557594354
12310	26-27	4.212386200144433
12310	28-29	4.825727836883331
12310	30-31	4.854500455512515
12310	32-33	5.9873076336285
12310	34-35	2.7621449610857596
12310	36-37	5.387152977349057
12310	38-39	-6.908525860644307
12310	40-41	5.615545320169652
12310	42-43	5.914520083791903
12310	44-45	-4.568749635873278
12310	46-47	6.209744637722665
12310	48-49	5.145754667680134
12310	50-51	7.37314622780254
12310	52-53	-6.346824694644649
12310	54-55	0.8547996457868408
12310	56-57	1.7003183210911104
12310	58-59	4.388642395793422
12310	60-61	4.746961726702871
12310	62-63	-7.852986610236844
12310	64-65	3.1359712023961173
12310	66-67	0.8909801232284629
12310	68-69	-3.9071382168232454
12310	70-71	-4.061036134147535
12310	72-73	-8.499701314262545
12310	74-75	-8.853733673971274
12310	76	13.265936370282343
12311	1	2.3844058708799416
12311	2	1.4416042741974913
12311	3	0.34800981827413224
12311	4	0.6224066580606724
12311	5	0.7108787918944124
12311	6	1.7314132846612083
12311	7	1.7141252786677086
12311	8	2.881951749923786
12311	9	2.446543002584214
12311	10-11	2.2442232936037243
12311	12-13	2.9227517165189525
12311	14-15	-7.709196951166973
12311	16-17	3.652626375918601
12311	18-19	4.090955537220093
12311	20-21	3.0892473432789416
12311	22-23	3.5517351616057624
12311	24-25	3.250307557594354
12311	26-27	2.212386200144433
12311	28-29	4.825727836883331
12311	30-31	0.8545004555125146
12311	32-33	3.9873076336285003
12311	34-35	4.76214496108576
12311	36-37	7.387152977349057
12311	38-39	6.091474139355693
12311	40-41	7.615545320169652
12311	42-43	5.914520083791903
12311	44-45	6.431250364126722
12311	46-47	1.7097446377226646
12311	48-49	-2.3542453323198664
12311	50-51	7.37314622780254
12311	52-53	2.153175305355351
12311	54-55	-5.645200354213159
12311	56-57	5.70031832109111
12311	58-59	1.888642395793422
12311	60-61	2.246961726702871
12311	62-63	3.1470133897631563
12311	64-65	7.135971202396117
12311	66-67	7.390980123228463
12311	68-69	-2.9071382168232454
12311	70-71	6.938963865852465
12311	72-73	10.000298685737455
12311	74-75	4.1462663260287265
12311	76	13.265936370282343
12312	1	-3.6155941291200584
12312	2	1.4416042741974913
12312	3	0.34800981827413224
12312	4	-5.377593341939328
12312	5	0.7108787918944124
12312	6	0.39807995132787255
12312	7	0.3807919453343729
12312	8	-11.784714916742882
12312	9	2.446543002584214
12312	10-11	1.577556626937053
12312	12-13	-5.0772482834810475
12312	14-15	2.6241363821663555
12312	16-17	2.9859597092519294
12312	18-19	4.090955537220093
12312	20-21	-1.2440859900543941
12312	22-23	2.2184018282724267
12312	24-25	-4.083025775738978
12312	26-27	2.212386200144433
12312	28-29	0.4923945035499955
12312	30-31	3.521167122179179
12312	32-33	2.320640966961836
12312	34-35	0.4288116277524239
12312	36-37	-1.2795136893176107
12312	38-39	7.424807472689022
12312	40-41	-0.7177880131636805
12312	42-43	-1.2521465828747615
12312	44-45	-3.068749635873278
12312	46-47	-1.123588695610671
12312	48-49	-0.8542453323198664
12312	50-51	-2.1268537721974603
12312	52-53	5.153175305355351
12312	54-55	1.6881329791201765
12312	56-57	-1.1330150122422253
12312	58-59	-5.611357604206578
12312	60-61	1.5802950600362067
12312	62-63	-2.8529866102368437
12312	64-65	-5.197362130937218
12312	66-67	-3.609019876771537
12312	68-69	2.9261951165100903
12312	70-71	-5.727702800814203
12312	72-73	-0.6663679809292091
12312	74-75	4.812932992695391
12312	76	8.265936370282343
13211	1	2.3844058708799416
13211	2	1.4416042741974913
13211	3	0.34800981827413224
13211	4	0.6224066580606724
13211	5	0.7108787918944124
13211	6	1.7314132846612083
13211	7	-2.2858747213322914
13211	8	-1.118048250076214
13211	9	2.446543002584214
13211	10-11	0.2442232936037243
13211	12-13	2.9227517165189525
13211	14-15	3.290803048833027
13211	16-17	3.652626375918601
13211	18-19	2.0909555372200934
13211	20-21	3.0892473432789416
13211	22-23	3.5517351616057624
13211	24-25	3.250307557594354
13211	26-27	2.212386200144433
13211	28-29	-8.174272163116669
13211	30-31	2.8545004555125146
13211	32-33	5.9873076336285
13211	34-35	4.76214496108576
13211	36-37	-3.612847022650943
13211	38-39	-2.908525860644307
13211	40-41	7.615545320169652
13211	42-43	-5.085479916208097
13211	44-45	-1.068749635873278
13211	46-47	4.209744637722665
13211	48-49	-1.8542453323198664
13211	50-51	-4.62685377219746
13211	52-53	-6.346824694644649
13211	54-55	5.354799645786841
13211	56-57	-7.29968167890889
13211	58-59	4.388642395793422
13211	60-61	-4.253038273297129
13211	62-63	5.147013389763156
13211	64-65	-3.8640287976038827
13211	66-67	-14.609019876771537
13211	68-69	-2.9071382168232454
13211	70-71	-4.061036134147535
13211	72-73	-5.499701314262545
13211	74-75	-5.3537336739712735
13211	76	-4.734063629717657
22305	1	2.3844058708799416
22305	2	1.4416042741974913
22305	3	0.34800981827413224
22305	4	0.6224066580606724
22305	5	0.7108787918944124
22305	6	-13.268586715338792
22305	7	-13.285874721332291
22305	8	-12.118048250076214
22305	9	-19.553456997415786
22305	10-11	-4.255776706396276
22305	12-13	0.9227517165189525
22305	14-15	-15.209196951166973
22305	16-17	1.6526263759186008
22305	18-19	-17.909044462779907
22305	20-21	-12.410752656721058
22305	22-23	3.5517351616057624
22305	24-25	-9.749692442405646
22305	26-27	0.212386200144433
22305	28-29	2.825727836883331
22305	30-31	-8.145499544487485
22305	32-33	-3.0126923663714997
22305	34-35	-10.73785503891424
22305	36-37	-11.112847022650943
22305	38-39	-1.408525860644307
22305	40-41	-4.384454679830348
22305	42-43	-0.5854799162080973
22305	44-45	6.431250364126722
22305	46-47	6.209744637722665
22305	48-49	5.145754667680134
22305	50-51	7.37314622780254
22305	52-53	6.653175305355351
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13308	54-55	5.354799645786841
13308	56-57	-9.79968167890889
13308	58-59	-15.611357604206578
13308	60-61	-4.253038273297129
13308	62-63	0.6470133897631563
13308	64-65	-14.864028797603883
13308	66-67	-14.609019876771537
13308	68-69	-10.407138216823245
13308	70-71	-4.061036134147535
13308	72-73	6.000298685737455
13308	74-75	9.146266326028726
13308	76	-18.734063629717657
13311	1	-15.615594129120058
13311	2	1.4416042741974913
13311	3	0.34800981827413224
13311	4	0.6224066580606724
13311	5	0.7108787918944124
13311	6	1.7314132846612083
13311	7	1.7141252786677086
13311	8	2.881951749923786
13311	9	2.446543002584214
13311	10-11	2.2442232936037243
13311	12-13	2.9227517165189525
13311	14-15	3.290803048833027
13311	16-17	3.652626375918601
13311	18-19	4.090955537220093
13311	20-21	3.0892473432789416
13311	22-23	3.5517351616057624
13311	24-25	3.250307557594354
13311	26-27	4.212386200144433
13311	28-29	2.825727836883331
13311	30-31	0.8545004555125146
13311	32-33	5.9873076336285
13311	34-35	4.76214496108576
13311	36-37	5.387152977349057
13311	38-39	4.091474139355693
13311	40-41	7.615545320169652
13311	42-43	5.914520083791903
13311	44-45	-2.568749635873278
13311	46-47	-3.2902553622773354
13311	48-49	5.145754667680134
13311	50-51	-3.6268537721974603
13311	52-53	6.653175305355351
13311	54-55	0.8547996457868408
13311	56-57	5.70031832109111
13311	58-59	6.388642395793422
13311	60-61	2.246961726702871
13311	62-63	5.147013389763156
13311	64-65	7.135971202396117
13311	66-67	3.390980123228463
13311	68-69	8.092861783176755
13311	70-71	6.938963865852465
13311	72-73	3.500298685737455
13311	74-75	1.1462663260287265
13311	76	13.265936370282343
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	1.0
17	4.0
18	9.0
19	14.0
20	34.0
21	67.0
22	154.0
23	287.0
24	426.0
25	607.0
26	963.0
27	1412.0
28	2320.0
29	3497.0
30	5033.0
31	6298.0
32	6570.0
33	4935.0
34	1340.0
35	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.794809169290527	22.56128064032016	29.48533090074449	17.158579289644823
2	36.06779262049079	20.343670923321366	23.845112693462013	19.74342376272583
3	36.41499440946272	18.204554816689225	22.777025834167013	22.603424939681044
4	39.95762961219326	19.63749779320897	20.793856293768023	19.611016300829753
5	34.36414994409463	23.580297769669865	21.008650620843877	21.046901665391633
6	33.40198905431648	19.575707644324133	20.564350026481492	26.457953274877895
7	29.60630847996234	18.469369740481376	23.186017772023774	28.738304007532513
8	27.18766550932737	14.582475136821044	28.885423409639266	29.34443594421232
9	25.463426116636263	15.812393338433473	26.813982227976226	31.91019831695404
10-11	27.92996910401648	22.65116963366191	25.626011475651023	23.79284978667059
12-13	25.01765432825281	29.20025893014771	29.01047490143	16.77161184016948
14-15	19.633084211145764	29.77402459836403	30.208026834578945	20.384864355911258
16-17	23.73624433590302	31.355558171011594	25.459012534573063	19.449184958512326
18-19	25.903313128935444	27.408344612487497	25.12946507385394	21.558877184723123
20-21	30.132995939504504	22.413640910963338	30.02412758194551	17.429235567586655
22-23	31.9336756462505	21.721667230649267	26.400270711647956	19.94438641145228
24-25	28.10293079096045	27.48793549905838	22.44438559322034	21.96474811676083
26-27	20.559111307290518	27.33171485323328	21.837710586331198	30.271463253145004
28-29	22.070507915955506	23.40650932846801	22.164675416396918	32.35830733917957
30-31	23.677085172758844	21.978927541350284	27.637000412031316	26.706986873859556
32-33	25.136129506990436	29.256806475349524	27.496688741721854	18.11037527593819
34-35	20.674888156345656	35.57952672474688	29.847539439604425	13.898045679303037
36-37	17.383625954760188	30.165271012082588	38.87768767752285	13.573415355634372
38-39	17.90071673510236	37.2349036749231	31.075691347668034	13.788688242306504
40-41	23.43331469107821	35.653312924969825	21.8629499896977	19.05042239425426
42-43	20.707620757660493	35.347187472404556	25.00809466340918	18.937097106525773
44-45	16.5427919640886	36.81654279196409	31.974391051585844	14.66627419236147
46-47	17.452351166384574	35.55964382956803	33.72727941717565	13.260725586871736
48-49	22.82400729991464	32.87610749713007	29.65884673122774	14.64103847172755
50-51	21.185093606499468	34.26498292711645	24.780701754385966	19.769221711998117
52-53	24.330649553275734	33.799437730905666	19.55136225143143	22.318550464387172
54-55	31.544622088743633	30.746694932720903	18.733621882636985	18.975061095898475
56-57	32.438600624300605	35.415807762530186	18.410978267271336	13.734613345897873
58-59	29.858329406220545	39.69427426955702	18.102026390197924	12.345369934024506
60-61	22.012550821990455	48.0849684756349	17.89965234812327	12.00282835425137
62-63	17.24778761061947	52.97345132743363	17.799410029498524	11.979351032448378
64-65	16.49659863945578	54.66725820763087	17.216799763383612	11.619343389529725
66-67	16.522061549870227	55.202076381164254	16.84835001853912	11.4275120504264
68-69	17.078210712533632	55.068607192233934	16.990500542613763	10.86268155261867
70-71	17.288418293645474	55.04772967579562	17.12907117008444	10.534780860474466
72-73	16.836124401913878	55.91656698564593	16.676136363636363	10.571172248803828
74-75	17.07670966404643	54.56734218792353	17.363713187811918	10.992234960218122
76	30.736201108595516	0.0	40.776016863143106	28.48778202826138
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	1.5
2	1.5
3	2.0
4	2.0
5	5.5
6	15.0
7	18.5
8	16.0
9	27.0
10	35.0
11	41.0
12	50.5
13	57.5
14	63.5
15	75.5
16	95.0
17	117.0
18	162.0
19	246.5
20	311.0
21	344.0
22	401.0
23	493.0
24	558.5
25	586.5
26	669.0
27	802.5
28	904.0
29	917.5
30	919.0
31	937.0
32	920.5
33	908.5
34	958.5
35	1058.5
36	1131.0
37	1145.5
38	1094.0
39	1248.5
40	1488.0
41	2407.5
42	3734.0
43	3339.5
44	2389.0
45	1718.0
46	959.5
47	706.5
48	637.0
49	592.5
50	558.5
51	564.0
52	554.0
53	538.5
54	560.0
55	547.0
56	508.0
57	503.0
58	508.0
59	497.5
60	504.5
61	500.0
62	467.5
63	427.5
64	349.5
65	293.5
66	271.0
67	247.0
68	204.5
69	139.5
70	94.5
71	81.5
72	90.5
73	92.0
74	79.5
75	75.0
76	64.5
77	47.5
78	31.5
79	20.5
80	24.5
81	23.5
82	14.0
83	7.0
84	3.0
85	1.5
86	0.5
87	0.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	4.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008827164126404989
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0029423880421349967
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.002943254061690605
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0014717569834868866
46-47	0.0014717569834868866
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0047831632653061226
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
20-21	1.0
22-23	1.0
24-25	1.0
26-27	1.0
28-29	3.0
30-31	3.0
32-33	0.0
34-35	1.0
36-37	1.0
38-39	1.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	1.0
50-51	2.0
52-53	4.0
54-55	8.0
56-57	4.0
58-59	9.0
60-61	32.0
62-63	74.0
64-65	99.0
66-67	92.0
68-69	59.0
70-71	82.0
72-73	927.0
74-75	19771.0
76-77	12809.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.00372694516908
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.47663274701023	76.38486455935994
2	2.3148673767738717	3.703960350513933
3	0.8234103026429882	1.9762767904946597
4	0.3624990147615997	1.1600508877951932
5	0.23256798080429844	0.9303152616228206
6	0.13234835186977548	0.6353016842779604
7	0.08162319903810007	0.45711120897647406
8	0.07514845648170869	0.48097252741708346
9	0.04642639393775149	0.33428560892802506
>10	0.381263591036703	5.64002443407928
>50	0.0439275515203693	2.3926987652414926
>100	0.025624404857325086	3.5963697258670595
>500	0.0036606292653321546	2.307768195426093
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	788	2.3186017772023773	No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAAA	312	0.9180250691461189	No Hit
AGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	188	0.5531689519213794	No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	185	0.5443417877949744	No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	151	0.44430059436238445	No Hit
TCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	148	0.4354734302359795	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAAAAAAAAAAAAAA	140	0.41193432589889956	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	104	0.30600835638203966	No Hit
GCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	96	0.28246925204495965	No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	84	0.24716059553933972	No Hit
AAGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	80	0.23539104337079975	No Hit
TAGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	71	0.20890955099158479	No Hit
ATGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	69	0.2030247749073148	No Hit
ATCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAA	68	0.20008238686517976	No Hit
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	65	0.1912552227387748	No Hit
GGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	60	0.1765432825280998	No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAAAAAAAAAAAAAAA	60	0.1765432825280998	No Hit
TACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAA	59	0.1736008944859648	No Hit
CGTGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	53	0.1559465662331548	No Hit
AACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAA	52	0.15300417819101983	No Hit
GGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGC	44	0.12946507385393985	No Hit
GGGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTG	43	0.12652268581180487	No Hit
CAGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	38	0.11181074560112987	No Hit
TTCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAA	35	0.10298358147472489	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10	0.0	0.0	0.0
11	0.0	0.0	0.0
12	0.0	0.0	0.0
13	0.0	0.0	0.0
14	0.0	0.0	0.0
15	0.0	0.0	0.0
16	0.0	0.0	0.0
17	0.0	0.0	0.0
18	0.0	0.0	0.0
19	0.0	0.0	0.0
20	0.0	0.0	0.0
21	0.0	0.0	0.0
22	0.0	0.0	0.0
23	0.0	0.0	0.0
24	0.0	0.0	0.0
25	0.005884776084269993	0.0	0.0
26	0.00882716412640499	0.0	0.0
27	0.02648149237921497	0.0	0.0
28	0.047078208674159946	0.0	0.0
29	0.06767492496910492	0.0	0.0
30	0.0912140293061849	0.0	0.0
31	0.15888895427528982	0.0	0.0
32	0.20302477490731477	0.0	0.0
33	0.24127581945506973	0.0	0.0
34	0.2648149237921497	0.0	0.0
35	0.28835402812922967	0.0	0.0
36	0.33248984876125465	0.0	0.0
37	0.3648561172247396	0.0	0.0
38	0.4001647737303596	0.0	0.0
39	0.41487671394103454	0.0	0.0
40	0.4501853704466545	0.0	0.0
41	0.48255163891013947	0.0	0.0
42	0.5266874595421644	0.0	0.0
43	0.5531689519213794	0.0	0.0
44	0.5796504443005943	0.0	0.0
45	0.6237862649326193	0.0	0.0
46	0.6443829812275643	0.0	0.0
47	0.6649796975225093	0.0	0.0
48	0.7032307420702643	0.0	0.0
49	0.7591361148708292	0.0	0.0
50	0.8238686517977991	0.0	0.0
51	0.856234920261284	0.0	0.0
52	0.909197905019714	0.0	0.0
53	0.9533337256517389	0.0	0.0
54	0.9974695462837639	0.0	0.0
55	1.0563173071264638	0.0	0.0
56	1.1328193962219737	0.0	0.0
57	1.2152062614017536	0.0	0.0
58	1.2740540222444536	0.0	0.0
59	1.3534984993820984	0.0	0.0
60	1.4182310363090684	0.0	0.0
61	1.4417701406461485	0.0	0.0
62	1.4711940210674983	0.0	0.0
63	1.4829635732360384	0.0	0.0
64	1.4829635732360384	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCTGTA	15	0.0023332909	69.01914	8
ATCCGAC	15	0.0023332909	69.01914	2
ATTATGT	45	0.003575641	61.744404	70
TTTACTT	60	1.4200866E-4	61.744396	70
CGCCCCT	25	2.528288E-4	55.21532	7
CCGCCCC	25	2.528288E-4	55.21532	6
GCTGTAG	25	2.528288E-4	55.21532	9
CCCGCCC	20	0.0072900024	51.76436	5
CCGACCT	30	6.2163745E-4	46.01276	4
CCGAAGC	35	0.0012248923	40.088966	62
CGGAGAA	35	0.0013180171	39.497684	53
TGTCCCG	35	0.0013180171	39.497684	49
GTCCCGC	35	0.0013180171	39.497684	50
AGTGTCC	35	0.0013180171	39.497684	47
CCTTGCC	35	0.0013276299	39.43951	11
CCCTTGC	35	0.0013276299	39.43951	10
GCCCCTT	35	0.0013276299	39.43951	8
CGTTTAC	40	0.0020015363	36.272446	68
GCGTTTA	40	0.002274543	35.342384	67
AGCGTTT	40	0.0023256398	35.18318	66
>>END_MODULE
