##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	OU_LexBC004_S44_L001_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9950
Sequences flagged as poor quality	0
Sequence length	52-76
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17286432160804	32.0	32.0	32.0	32.0	32.0
2	30.918693467336684	32.0	32.0	32.0	32.0	32.0
3	30.95095477386935	32.0	32.0	32.0	32.0	32.0
4	30.791557788944722	32.0	32.0	32.0	32.0	32.0
5	30.76683417085427	32.0	32.0	32.0	32.0	32.0
6	33.950653266331656	36.0	36.0	36.0	32.0	36.0
7	33.89608040201005	36.0	36.0	36.0	32.0	36.0
8	33.791959798994974	36.0	36.0	36.0	32.0	36.0
9	33.69688442211055	36.0	36.0	36.0	27.0	36.0
10-11	33.811557788944725	36.0	36.0	36.0	32.0	36.0
12-13	33.29125628140704	36.0	36.0	36.0	27.0	36.0
14-15	32.91155778894472	36.0	32.0	36.0	21.0	36.0
16-17	32.844070351758795	36.0	32.0	36.0	21.0	36.0
18-19	32.97537688442211	36.0	32.0	36.0	27.0	36.0
20-21	32.80713567839196	36.0	32.0	36.0	24.0	36.0
22-23	32.77859296482412	36.0	32.0	36.0	24.0	36.0
24-25	32.63452261306533	36.0	32.0	36.0	17.5	36.0
26-27	31.992964824120605	36.0	32.0	36.0	14.0	36.0
28-29	31.698442211055276	36.0	32.0	36.0	14.0	36.0
30-31	31.12427135678392	36.0	29.5	36.0	14.0	36.0
32-33	30.550201005025123	34.0	27.0	36.0	14.0	36.0
34-35	31.302663316582915	36.0	32.0	36.0	14.0	36.0
36-37	31.040100502512562	36.0	29.5	36.0	14.0	36.0
38-39	28.4008040201005	32.0	24.0	34.0	14.0	36.0
40-41	28.200603015075377	32.0	24.0	34.0	14.0	36.0
42-43	28.934271356783917	32.0	27.0	36.0	14.0	36.0
44-45	29.395829145728644	32.0	27.0	36.0	14.0	36.0
46-47	29.612613065326634	32.0	27.0	36.0	14.0	36.0
48-49	30.16180904522613	32.0	27.0	36.0	14.0	36.0
50-51	29.834422110552765	34.0	27.0	36.0	14.0	36.0
52-53	30.05598592015258	36.0	27.0	36.0	14.0	36.0
54-55	31.07267062016283	36.0	32.0	36.0	14.0	36.0
56-57	31.195949341642375	36.0	32.0	36.0	14.0	36.0
58-59	30.608828431716674	36.0	27.0	36.0	14.0	36.0
60-61	29.5589063128267	34.0	27.0	36.0	14.0	36.0
62-63	29.17386154534042	34.0	27.0	36.0	14.0	36.0
64-65	29.360018866920733	32.0	27.0	36.0	14.0	36.0
66-67	28.66322303862996	32.0	21.0	36.0	14.0	36.0
68-69	27.715190551584065	32.0	17.5	36.0	14.0	36.0
70-71	27.083387873830073	32.0	14.0	36.0	14.0	36.0
72-73	25.118761117844663	27.0	14.0	32.0	14.0	36.0
74-75	24.52021866421682	27.0	14.0	32.0	14.0	34.0
76	25.380116959064328	27.0	14.0	32.0	14.0	36.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
11301	1	-0.4651523584733326
11301	2	1.622142061030818
11301	3	1.376673669572142
11301	4	1.3327014011236535
11301	5	-0.4766489580694433
11301	6	1.46530646927323
11301	7	0.21498262129361478
11301	8	2.1321653997363406
11301	9	3.3898410285128975
11301	10-11	0.16881560512109672
11301	12-13	1.3724838569656015
11301	14-15	0.7409790290979856
11301	16-17	0.2649370700910936
11301	18-19	0.732227457666383
11301	20-21	-1.1809527756883966
11301	22-23	2.1266086868182796
11301	24-25	0.3089124415287543
11301	26-27	0.8777711119423657
11301	28-29	1.2092388392292968
11301	30-31	1.7748197995557575
11301	32-33	1.0963076671238348
11301	34-35	2.260383006952207
11301	36-37	0.4762970332767331
11301	38-39	-1.670837035028672
11301	40-41	0.17071024163592696
11301	42-43	1.3811745000638354
11301	44-45	-0.5634133330516349
11301	46-47	1.2183848538877378
11301	48-49	1.1020110302229078
11301	50-51	1.9389159052091358
11301	52-53	0.7701001032968229
11301	54-55	0.8722197525541624
11301	56-57	1.168031033444798
11301	58-59	1.6876246706702993
11301	60-61	2.5123679192713055
11301	62-63	4.357547641145931
11301	64-65	1.8705986824125773
11301	66-67	0.31319494703968687
11301	68-69	1.8819960347771172
11301	70-71	-0.5274737422706259
11301	72-73	-0.3125199145877211
11301	74-75	2.4183807495086533
11301	76	7.557086153138787
12101	1	0.2799456807423546
12101	2	2.2593969629916018
12101	3	-6.45665966376119
12101	4	2.793485714849144
12101	5	0.3860961399697729
12101	6	3.4849143124104884
12101	7	2.08753164090146
12101	8	4.6027536350304565
12101	9	-2.453296226389064
12101	10-11	-3.87040008115342
12101	12-13	-1.5981043783285145
12101	14-15	2.3537241271371983
12101	16-17	3.1325841289146226
12101	18-19	2.2371294184506922
12101	20-21	-0.4162468933354546
12101	22-23	4.366804765249647
12101	24-25	-2.519518931020265
12101	26-27	3.353261308020791
12101	28-29	-2.697623905868742
12101	30-31	-0.8379252984834586
12101	32-33	-0.021339391699694232
12101	34-35	-2.5092248361850444
12101	36-37	2.162571543080656
12101	38-39	3.0399472786968182
12101	40-41	0.6756122024202398
12101	42-43	5.5821548922207
12101	44-45	1.8189396081248361
12101	46-47	-0.7080857343475557
12101	48-49	0.8373051478699658
12101	50-51	0.5320531601110972
12101	52-53	1.2112765738850584
12101	54-55	2.2545726937306334
12101	56-57	-0.5623611234179471
12101	58-59	-2.1653165058002877
12101	60-61	6.78197576240856
12101	62-63	-6.711079809834462
12101	64-65	1.5617751530008093
12101	66-67	-4.6770011313916875
12101	68-69	-1.0338455493812972
12101	70-71	1.1532193270363074
12101	72-73	3.6198563230360428
12101	74-75	-2.18284837850619
12101	76	-17.46994087388824
12102	1	0.2799456807423546
12102	2	2.2593969629916018
12102	3	2.54334033623881
12102	4	-8.206514285150856
12102	5	0.3860961399697729
12102	6	3.4849143124104884
12102	7	2.08753164090146
12102	8	0.6027536350304565
12102	9	1.0467037736109361
12102	10-11	0.62959991884658
12102	12-13	3.9018956216714855
12102	14-15	3.3537241271371983
12102	16-17	-1.3674158710853774
12102	18-19	3.2371294184506922
12102	20-21	0.8337531066645454
12102	22-23	-5.633195234750353
12102	24-25	-7.019518931020265
12102	26-27	4.353261308020791
12102	28-29	5.052376094131258
12102	30-31	4.662074701516541
12102	32-33	6.478660608300306
12102	34-35	6.240775163814956
12102	36-37	-5.587428456919344
12102	38-39	4.289947278696818
12102	40-41	-3.82438779757976
12102	42-43	3.0821548922207
12102	44-45	6.068939608124836
12102	46-47	-2.4580857343475557
12102	48-49	-3.662694852130034
12102	50-51	-5.467946839888903
12102	52-53	2.2112765738850584
12102	54-55	-1.2454273062693666
12102	56-57	5.937638876582053
12102	58-59	7.834683494199712
12102	60-61	-3.9680242375914396
12102	62-63	-1.2110798098344624
12102	64-65	6.061775153000809
12102	66-67	4.3229988686083125
12102	68-69	-3.0338455493812972
12102	70-71	2.4032193270363074
12102	72-73	-0.8801436769639572
12102	74-75	8.81715162149381
12102	76	14.530059126111759
11305	1	0.2799456807423546
11305	2	2.2593969629916018
11305	3	-15.45665966376119
11305	4	2.793485714849144
11305	5	0.3860961399697729
11305	6	3.4849143124104884
11305	7	2.08753164090146
11305	8	-17.397246364969543
11305	9	-16.953296226389064
11305	10-11	-4.87040008115342
11305	12-13	3.9018956216714855
11305	14-15	3.3537241271371983
11305	16-17	3.1325841289146226
11305	18-19	1.2371294184506922
11305	20-21	2.8337531066645454
11305	22-23	0.8668047652496469
11305	24-25	-3.519518931020265
11305	26-27	2.353261308020791
11305	28-29	0.5523760941312581
11305	30-31	-6.337925298483459
11305	32-33	-0.021339391699694232
11305	34-35	1.7407751638149556
11305	36-37	-10.087428456919344
11305	38-39	-3.2100527213031818
11305	40-41	-3.82438779757976
11305	42-43	5.5821548922207
11305	44-45	1.5689396081248361
11305	46-47	-2.4580857343475557
11305	48-49	-8.162694852130034
11305	50-51	-3.467946839888903
11305	52-53	6.711276573885058
11305	54-55	-5.745427306269367
11305	56-57	5.937638876582053
11305	58-59	0.33468349419971233
11305	60-61	1.5319757624085604
11305	62-63	-5.711079809834462
11305	64-65	-2.9382248469991907
11305	66-67	-0.6770011313916875
11305	68-69	-12.033845549381297
11305	70-71	-3.0967806729636926
11305	72-73	3.6198563230360428
11305	74-75	11.31715162149381
11305	76	14.530059126111759
11308	1	0.2799456807423546
11308	2	2.2593969629916018
11308	3	2.54334033623881
11308	4	-15.206514285150856
11308	5	0.3860961399697729
11308	6	-18.51508568758951
11308	7	2.08753164090146
11308	8	4.6027536350304565
11308	9	5.046703773610936
11308	10-11	0.62959991884658
11308	12-13	-7.0981043783285145
11308	14-15	-7.646275872862802
11308	16-17	3.1325841289146226
11308	18-19	1.2371294184506922
11308	20-21	2.8337531066645454
11308	22-23	-7.633195234750353
11308	24-25	-9.019518931020265
11308	26-27	-2.146738691979209
11308	28-29	-5.947623905868742
11308	30-31	-6.337925298483459
11308	32-33	-5.521339391699694
11308	34-35	4.240775163814956
11308	36-37	3.412571543080656
11308	38-39	4.289947278696818
11308	40-41	5.17561220242024
11308	42-43	7.5821548922207
11308	44-45	-4.931060391875164
11308	46-47	-2.4580857343475557
11308	48-49	-3.662694852130034
11308	50-51	7.532053160111097
11308	52-53	-4.288723426114942
11308	54-55	-1.2454273062693666
11308	56-57	-1.562361123417947
11308	58-59	-7.665316505800288
11308	60-61	-12.96802423759144
11308	62-63	9.788920190165538
11308	64-65	3.5617751530008093
11308	66-67	-2.6770011313916875
11308	68-69	-5.533845549381297
11308	70-71	-1.0967806729636926
11308	72-73	-5.380143676963957
11308	74-75	-8.68284837850619
11308	76	9.530059126111759
13101	1	0.2799456807423546
13101	2	2.2593969629916018
13101	3	2.54334033623881
13101	4	2.793485714849144
13101	5	0.3860961399697729
13101	6	3.4849143124104884
13101	7	2.08753164090146
13101	8	4.6027536350304565
13101	9	5.046703773610936
13101	10-11	2.62959991884658
13101	12-13	3.9018956216714855
13101	14-15	3.3537241271371983
13101	16-17	3.1325841289146226
13101	18-19	3.2371294184506922
13101	20-21	-1.6662468933354546
13101	22-23	-7.633195234750353
13101	24-25	3.980481068979735
13101	26-27	2.353261308020791
13101	28-29	5.052376094131258
13101	30-31	6.662074701516541
13101	32-33	6.478660608300306
13101	34-35	4.240775163814956
13101	36-37	5.412571543080656
13101	38-39	-4.710052721303182
13101	40-41	7.17561220242024
13101	42-43	-5.4178451077793
13101	44-45	4.068939608124836
13101	46-47	-2.4580857343475557
13101	48-49	7.337305147869966
13101	50-51	7.532053160111097
13101	52-53	6.711276573885058
13101	54-55	5.254572693730633
13101	56-57	5.937638876582053
13101	58-59	-5.165316505800288
13101	60-61	7.03197576240856
13101	62-63	5.788920190165538
13101	64-65	3.5617751530008093
13101	66-67	3.8229988686083125
13101	68-69	7.966154450618703
13101	70-71	5.403219327036307
13101	72-73	5.619856323036043
13101	74-75	-22.68284837850619
13101	76	-17.46994087388824
21101	1	-0.33249451064520485
21101	2	1.2067653840442318
21101	3	1.4643929678177585
21101	4	1.3030550928395748
21101	5	-0.9057698887383623
21101	6	1.8700817765253177
21101	7	0.24303403324595507
21101	8	2.332418706800791
21101	9	2.3624932472951485
21101	10-11	0.5195520719566247
21101	12-13	1.155484138417897
21101	14-15	-0.021873958987200126
21101	16-17	-0.4116742442911203
21101	18-19	-0.08464091647753946
21101	20-21	-0.6722277545794739
21101	22-23	2.0665655307998847
21101	24-25	1.1467490115634646
21101	26-27	0.9190507817050033
21101	28-29	0.38850049604513615
21101	30-31	2.2936536488849626
21101	32-33	1.1676558236113088
21101	34-35	2.056564637499168
21101	36-37	1.1422366148509902
21101	38-39	-1.3930670753701655
21101	40-41	-0.8040528693500946
21101	42-43	1.181437188871417
21101	44-45	0.22803051721574263
21101	46-47	2.9163161795280423
21101	48-49	2.284673568922596
21101	50-51	2.1313354567618177
21101	52-53	1.1921378179041966
21101	54-55	0.9172521195679515
21101	56-57	1.1469690201227216
21101	58-59	2.479420336304976
21101	60-61	3.1791049490114283
21101	62-63	4.03652784566793
21101	64-65	2.143114865919472
21101	66-67	1.3195451722352693
21101	68-69	2.6621645780850898
21101	70-71	2.10944772920843
21101	72-73	-0.707801783198974
21101	74-75	1.1408133994363112
21101	76	8.05978885584149
23309	1	-1.6281002962691389
23309	2	1.1214659285088437
23309	3	1.876673669572142
23309	4	1.9658995079525923
23309	5	-0.280570526696895
23309	6	1.8757189101116367
23309	7	0.19097991676353132
23309	8	3.2579260488235597
23309	9	2.138657796599439
23309	10-11	0.10661141309945776
23309	12-13	2.120286426269189
23309	14-15	-0.07731035562142097
23309	16-17	-0.15477219292446165
23309	18-19	0.31184206212885357
23309	20-21	-0.2467066634503965
23309	22-23	2.378299018123208
23309	24-25	-0.1574499655030266
23309	26-27	-0.8536352437033443
23309	28-29	0.12134161137263888
23309	30-31	1.5816149314015995
23309	32-33	1.409695091058925
23309	34-35	1.2522694166885202
23309	36-37	-0.9552445488733703
23309	38-39	-2.6928113419928366
23309	40-41	-1.985307337809644
23309	42-43	-0.6304887859402193
23309	44-45	-1.5689914263579219
23309	46-47	0.27754644956048935
23309	48-49	0.05569595246766923
23309	50-51	1.4975704014904068
23309	52-53	-0.2714820468046
23309	54-55	1.0131933833858078
23309	56-57	1.1387883018694076
23309	58-59	2.467669326864776
23309	60-61	3.787789715896931
23309	62-63	2.983037837224362
23309	64-65	0.8617751530008064
23309	66-67	0.15829298625537191
23309	68-69	0.14850739179517092
23309	70-71	-0.6320747906107513
23309	72-73	0.1526294322797419
23309	74-75	2.2218813512235407
23309	76	6.345848599795968
13102	1	0.2799456807423546
13102	2	2.2593969629916018
13102	3	2.54334033623881
13102	4	2.793485714849144
13102	5	0.3860961399697729
13102	6	3.4849143124104884
13102	7	2.08753164090146
13102	8	0.6027536350304565
13102	9	5.046703773610936
13102	10-11	2.62959991884658
13102	12-13	-7.0981043783285145
13102	14-15	3.3537241271371983
13102	16-17	-7.867415871085377
13102	18-19	3.2371294184506922
13102	20-21	2.8337531066645454
13102	22-23	5.366804765249647
13102	24-25	3.980481068979735
13102	26-27	4.353261308020791
13102	28-29	-5.947623905868742
13102	30-31	2.1620747015165414
13102	32-33	-4.521339391699694
13102	34-35	4.240775163814956
13102	36-37	3.412571543080656
13102	38-39	-2.7100527213031818
13102	40-41	7.17561220242024
13102	42-43	-14.4178451077793
13102	44-45	-2.931060391875164
13102	46-47	-13.458085734347556
13102	48-49	3.337305147869966
13102	50-51	-3.467946839888903
13102	52-53	-0.7887234261149416
13102	54-55	5.254572693730633
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13311	36-37	2.4403493208584273
13311	38-39	-1.8767193879698496
13311	40-41	-1.1299433531353174
13311	42-43	0.22104378110958933
13311	44-45	-0.20883816965294244
13311	46-47	0.20858093231911212
13311	48-49	-0.24602818546336636
13311	50-51	-0.9123912843333457
13311	52-53	0.8501654627739477
13311	54-55	-1.0509828618249237
13311	56-57	0.3543055432487172
13311	58-59	0.3624612719774909
13311	60-61	0.5319757624085604
13311	62-63	2.4555868568322055
13311	64-65	0.47844181966747357
13311	66-67	0.8785544241638696
13311	68-69	1.882821117285367
13311	70-71	0.5698859937029717
13311	72-73	-1.6301436769639572
13311	74-75	4.608001294696425
13311	76	6.613392459445091
22111	1	0.2799456807423546
22111	2	-15.740603037008398
22111	3	2.54334033623881
22111	4	2.793485714849144
22111	5	0.3860961399697729
22111	6	-11.515085687589512
22111	7	2.08753164090146
22111	8	4.6027536350304565
22111	9	-16.953296226389064
22111	10-11	0.62959991884658
22111	12-13	-7.0981043783285145
22111	14-15	3.3537241271371983
22111	16-17	3.1325841289146226
22111	18-19	-7.762870581549308
22111	20-21	2.8337531066645454
22111	22-23	5.366804765249647
22111	24-25	3.980481068979735
22111	26-27	-17.64673869197921
22111	28-29	5.052376094131258
22111	30-31	-2.3379252984834586
22111	32-33	-4.521339391699694
22111	34-35	-15.759224836185044
22111	36-37	-5.587428456919344
22111	38-39	-4.710052721303182
22111	40-41	7.17561220242024
22111	42-43	-3.4178451077793
22111	44-45	-4.931060391875164
22111	46-47	4.041914265652444
22111	48-49	-14.662694852130034
22111	50-51	-5.467946839888903
22111	52-53	-8.788723426114942
22111	54-55	3.2545726937306334
22111	56-57	1.437638876582053
22111	58-59	-14.165316505800288
22111	60-61	-1.9680242375914396
22111	62-63	7.788920190165538
22111	64-65	-2.9382248469991907
22111	66-67	-13.677001131391687
22111	68-69	7.966154450618703
22111	70-71	-3.0967806729636926
22111	72-73	3.6198563230360428
22111	74-75	8.81715162149381
22111	76	9.530059126111759
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	13.0
20	20.0
21	50.0
22	97.0
23	109.0
24	169.0
25	248.0
26	367.0
27	467.0
28	734.0
29	1003.0
30	1447.0
31	1834.0
32	1919.0
33	1185.0
34	284.0
35	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.914572864321606	16.763819095477388	36.76381909547739	21.55778894472362
2	37.66834170854271	17.758793969849247	28.130653266331656	16.44221105527638
3	34.37185929648241	16.71356783919598	26.402010050251256	22.512562814070353
4	41.89949748743719	17.386934673366834	23.20603015075377	17.507537688442213
5	35.63819095477387	23.98994974874372	21.889447236180903	18.482412060301506
6	33.949748743718594	18.321608040201003	21.417085427135678	26.31155778894472
7	30.462311557788947	17.42713567839196	23.618090452261306	28.492462311557787
8	27.979899497487438	14.71356783919598	30.301507537688444	27.00502512562814
9	26.66331658291457	15.1356783919598	26.703517587939697	31.497487437185928
10-11	28.42211055276382	22.65326633165829	25.804020100502512	23.12060301507538
12-13	23.839195979899497	30.366834170854272	29.47236180904523	16.321608040201006
14-15	19.764812302125733	30.890999547715964	29.890949293934373	19.45323885622393
16-17	23.839195979899497	32.10050251256281	26.487437185929647	17.57286432160804
18-19	25.542713567839197	27.894472361809047	25.949748743718594	20.613065326633166
20-21	28.28643216080402	23.894472361809047	31.100502512562816	16.71859296482412
22-23	30.361809045226128	22.738693467336685	27.52763819095477	19.371859296482413
24-25	27.306532663316585	29.251256281407034	23.35678391959799	20.08542713567839
26-27	19.809045226130653	27.889447236180903	23.55778894472362	28.74371859296482
28-29	21.763819095477388	24.959798994974875	22.844221105527637	30.432160804020103
30-31	21.829145728643216	24.030150753768844	28.442211055276385	25.698492462311556
32-33	24.49369315040957	30.65480677420976	27.31795567616463	17.53354439921604
34-35	20.424163232485675	36.119207960599056	30.033169162729923	13.423459644185346
36-37	17.10136187748128	30.830695009799484	38.92155384692698	13.146389265792251
38-39	17.51846826473692	39.33363485602291	29.9914568571285	13.156440022111665
40-41	23.388109955274132	35.87114930398512	21.925724910799538	18.815015829941203
42-43	20.157804804502966	36.03879786913258	25.761383053573223	18.042014272791235
44-45	17.117298220926727	37.02382148959694	31.35491004121017	14.503970248266157
46-47	17.36180904522613	36.55778894472362	32.79396984924623	13.28643216080402
48-49	23.839195979899497	32.73869346733668	29.899497487437188	13.522613065326633
50-51	21.281407035175878	34.527638190954775	24.502512562814072	19.688442211055275
52-53	24.976129453741393	33.40871400572893	19.66933011709131	21.945826423438362
54-55	32.97818876268972	30.410091466479045	18.936576540355816	17.675143230475424
56-57	31.927831942908835	35.732234395416626	19.137601769022012	13.202331892652527
58-59	29.868824445896365	39.67934864552445	18.439965823993568	12.011861084585616
60-61	22.77844792923201	46.52191395255328	18.792722155207077	11.90691596300764
62-63	19.250691475986926	50.661302489313556	18.642192607493087	11.445813427206437
64-65	18.797825211437775	51.469995972613766	18.797825211437775	10.934353604510672
66-67	18.792894630601534	51.64513524424707	18.232741219216795	11.329228905934597
68-69	19.67395706763872	51.71628189550426	18.05386796273795	10.555893074119076
70-71	20.456505199086987	51.153943697692114	17.829064164341872	10.560486938879027
72-73	20.98337243700908	51.09660308068958	17.77007038661634	10.149954095684995
74-75	21.753035559410232	48.59063313096271	18.462705984388553	11.193625325238509
76	39.113810166441745	0.0	37.89923526765632	22.986954565901936
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	0.5
6	1.5
7	3.5
8	3.5
9	4.5
10	8.5
11	9.5
12	8.5
13	10.0
14	13.5
15	19.0
16	22.5
17	23.5
18	29.5
19	49.5
20	68.0
21	79.5
22	89.0
23	101.0
24	114.0
25	136.5
26	170.0
27	195.0
28	223.0
29	246.0
30	261.0
31	272.0
32	276.0
33	289.0
34	315.0
35	365.5
36	411.0
37	409.0
38	364.5
39	397.0
40	460.0
41	686.0
42	1087.0
43	1043.0
44	775.5
45	556.0
46	328.5
47	257.5
48	245.0
49	242.0
50	236.5
51	217.0
52	202.0
53	196.0
54	185.0
55	179.5
56	163.0
57	139.0
58	130.0
59	118.0
60	106.5
61	91.0
62	72.5
63	72.5
64	65.5
65	50.5
66	46.5
67	43.5
68	31.5
69	20.0
70	14.0
71	12.5
72	11.5
73	12.5
74	16.0
75	18.0
76	15.0
77	7.5
78	3.5
79	4.0
80	2.0
81	1.5
82	1.5
83	0.0
84	0.5
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.005025125628140704
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.005025125628140704
34-35	0.010050251256281407
36-37	0.005025125628140704
38-39	0.005025125628140704
40-41	0.005025125628140704
42-43	0.010050251256281407
44-45	0.010050251256281407
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0050625221485344
70-71	0.005072022722661797
72-73	0.0
74-75	0.01084010840108401
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
52	1.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	1.0
59	0.0
60	0.0
61	3.0
62	5.0
63	4.0
64	8.0
65	6.0
66	28.0
67	14.0
68	7.0
69	6.0
70	18.0
71	24.0
72	44.0
73	175.0
74	762.0
75	4398.0
76	4446.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.85762031428598
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.04205115255522	81.49899910908763
2	2.157946807810733	3.6623646175125724
3	0.6375942005605576	1.6231417975727553
4	0.2749811157598628	0.9333697245899664
5	0.18748464419000643	0.7954750375717285
6	0.17498552662444247	0.8909313227275393
7	0.0374968967173473	0.22273281972232578
8	0.04999586214011053	0.33940239094167074
9	0.03749689659826256	0.28637076730494654
>10	0.362470000445634	5.600139449453352
>50	0.02499793106521479	1.2197273422104475
>100	0.012498965532607395	2.927345621305074
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	276	2.7738693467336684	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	62	0.6231155778894473	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAAAAAAAAAAAAAA	53	0.5326633165829145	No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	40	0.4020100502512563	No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	39	0.39195979899497485	No Hit
AGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	37	0.37185929648241206	No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAAA	31	0.31155778894472363	No Hit
TCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	30	0.3015075376884422	No Hit
CGTGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	26	0.26130653266331655	No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	22	0.22110552763819094	No Hit
TAGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	20	0.20100502512562815	No Hit
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	18	0.18090452261306533	No Hit
TACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAA	18	0.18090452261306533	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC	17	0.1708542713567839	No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAAAAAAAAAAAAAAA	17	0.1708542713567839	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGAAAAAAAAAAAAAAAAAA	15	0.1507537688442211	No Hit
GCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	15	0.1507537688442211	No Hit
GTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGCA	14	0.1407035175879397	No Hit
AAGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	14	0.1407035175879397	No Hit
GGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGC	14	0.1407035175879397	No Hit
ATGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	14	0.1407035175879397	No Hit
ATCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAA	13	0.13065326633165827	No Hit
GGGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTG	13	0.13065326633165827	No Hit
GGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	12	0.1206030150753769	No Hit
TTCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAA	12	0.1206030150753769	No Hit
AACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAA	12	0.1206030150753769	No Hit
TTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGCAG	12	0.1206030150753769	No Hit
GTCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAA	11	0.11055276381909547	No Hit
CGTGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	11	0.11055276381909547	No Hit
CGCGTACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	11	0.11055276381909547	No Hit
TTGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	10	0.10050251256281408	No Hit
AGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	10	0.10050251256281408	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10	0.0	0.0	0.0
11	0.0	0.0	0.0
12	0.0	0.0	0.0
13	0.0	0.0	0.0
14	0.0	0.0	0.0
15	0.0	0.0	0.0
16	0.0	0.0	0.0
17	0.0	0.0	0.0
18	0.0	0.0	0.0
19	0.0	0.0	0.0
20	0.0	0.0	0.0
21	0.0	0.0	0.0
22	0.0	0.0	0.0
23	0.010050251256281407	0.0	0.0
24	0.04020100502512563	0.0	0.0
25	0.05025125628140704	0.0	0.0
26	0.07035175879396985	0.0	0.0
27	0.08040201005025126	0.0	0.0
28	0.11055276381909548	0.0	0.0
29	0.16080402010050251	0.0	0.0
30	0.24120603015075376	0.0	0.0
31	0.2914572864321608	0.0	0.0
32	0.3316582914572864	0.0	0.0
33	0.4120603015075377	0.0	0.0
34	0.49246231155778897	0.0	0.0
35	0.542713567839196	0.0	0.0
36	0.6030150753768844	0.0	0.0
37	0.6331658291457286	0.0	0.0
38	0.6432160804020101	0.0	0.0
39	0.6834170854271356	0.0	0.0
40	0.7236180904522613	0.0	0.0
41	0.7537688442211056	0.0	0.0
42	0.8040201005025126	0.0	0.0
43	0.8442211055276382	0.0	0.0
44	0.8743718592964824	0.0	0.0
45	0.8844221105527639	0.0	0.0
46	0.9346733668341709	0.0	0.0
47	0.9748743718592965	0.0	0.0
48	1.035175879396985	0.0	0.0
49	1.085427135678392	0.0	0.0
50	1.135678391959799	0.0	0.0
51	1.1758793969849246	0.0	0.0
52	1.2160804020100502	0.0	0.0
53	1.2261306532663316	0.0	0.0
54	1.2964824120603016	0.0	0.0
55	1.3768844221105527	0.0	0.0
56	1.4170854271356783	0.0	0.0
57	1.4773869346733668	0.0	0.0
58	1.5175879396984924	0.0	0.0
59	1.5477386934673367	0.0	0.0
60	1.6080402010050252	0.0	0.0
61	1.6180904522613064	0.0	0.0
62	1.6884422110552764	0.0	0.0
63	1.7185929648241205	0.0	0.0
64	1.7185929648241205	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGCGA	15	0.0022624158	69.190956	1
TCGCGAC	15	0.0022624158	69.190956	2
CGCGACC	75	6.82121E-10	41.514572	1
TGCGACC	35	0.0012671615	39.53769	1
GCGACCT	140	0.0	32.124374	2
CGACCTC	165	0.0	31.450436	3
GACCTCA	195	0.0	26.611904	4
ATTAGTC	210	0.0	26.358458	44
CCTCAGA	210	0.0	26.358458	6
ACCTCAG	210	0.0	26.358458	5
CATATTA	210	0.0	26.358458	41
ATATTAG	210	0.0	26.358458	42
TTAGTCA	210	0.0	26.358458	45
GCATATT	210	0.0	26.358458	40
CGCTGAA	215	0.0	25.74547	28
CGGAGGA	215	0.0	25.74547	53
GCGACCC	215	0.0	25.74547	22
TAAGCAT	215	0.0	25.74547	37
CCGCTGA	215	0.0	25.74547	27
ACCCGCT	215	0.0	25.74547	25
>>END_MODULE
