##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	OU_LexBC003_S43_L004_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20695
Sequences flagged as poor quality	0
Sequence length	19-76
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.147136989611017	32.0	32.0	32.0	32.0	32.0
2	30.919110896351775	32.0	32.0	32.0	32.0	32.0
3	31.132012563421117	32.0	32.0	32.0	32.0	32.0
4	31.091423049045662	32.0	32.0	32.0	32.0	32.0
5	30.915728436820487	32.0	32.0	32.0	32.0	32.0
6	34.378932109205124	36.0	36.0	36.0	32.0	36.0
7	34.478231456873644	36.0	36.0	36.0	32.0	36.0
8	34.449335588306354	36.0	36.0	36.0	32.0	36.0
9	34.472239671418215	36.0	36.0	36.0	32.0	36.0
10-11	34.33143271321575	36.0	36.0	36.0	32.0	36.0
12-13	33.95324957719256	36.0	36.0	36.0	32.0	36.0
14-15	33.510388982846095	36.0	36.0	36.0	27.0	36.0
16-17	33.40475960376902	36.0	36.0	36.0	27.0	36.0
18-19	33.33462188934526	36.0	36.0	36.0	27.0	36.0
20-21	33.12423890982893	36.0	32.0	36.0	27.0	36.0
22-23	33.565467258254735	36.0	36.0	36.0	27.0	36.0
24-25	33.40151258879814	36.0	36.0	36.0	27.0	36.0
26-27	33.05390424455482	36.0	34.0	36.0	24.0	36.0
28-29	32.49830836152731	36.0	32.0	36.0	17.5	36.0
30-31	31.570520566484603	36.0	29.5	36.0	14.0	36.0
32-33	30.85842718352748	34.0	29.5	36.0	14.0	36.0
34-35	31.5464498042438	36.0	32.0	36.0	14.0	36.0
36-37	31.075813234085743	36.0	29.5	36.0	14.0	36.0
38-39	29.672047948185025	32.0	27.0	36.0	14.0	36.0
40-41	28.68625839818261	32.0	24.0	36.0	14.0	36.0
42-43	29.175793188680508	32.0	27.0	36.0	14.0	36.0
44-45	29.61944699569778	32.0	27.0	36.0	14.0	36.0
46-47	29.142794992023976	32.0	27.0	36.0	14.0	36.0
48-49	29.892323681539132	34.0	27.0	36.0	14.0	36.0
50-51	29.911176100932956	34.0	27.0	36.0	14.0	36.0
52-53	30.243986862153335	36.0	27.0	36.0	14.0	36.0
54-55	30.748699067075556	36.0	27.0	36.0	14.0	36.0
56-57	30.666747007247864	36.0	32.0	36.0	14.0	36.0
58-59	30.182850417349282	36.0	27.0	36.0	14.0	36.0
60-61	29.66030699276834	36.0	27.0	36.0	14.0	36.0
62-63	29.568897912057544	36.0	27.0	36.0	14.0	36.0
64-65	29.266135928292943	34.0	27.0	36.0	14.0	36.0
66-67	28.888007045365075	32.0	21.0	36.0	14.0	36.0
68-69	27.87298143562228	32.0	14.0	36.0	14.0	36.0
70-71	27.326992463930708	32.0	14.0	36.0	14.0	36.0
72-73	26.327460575207915	29.5	14.0	34.0	14.0	36.0
74-75	25.235830292852494	27.0	14.0	32.0	14.0	36.0
76	25.77419674732249	27.0	14.0	32.0	14.0	36.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
13509	1	0.8899649198802599
13509	2	0.7828696317946537
13509	3	-16.264850742791758
13509	4	1.4982260924277817
13509	5	1.5622441538892247
13509	6	1.2966021895716011
13509	7	2.8425834429251964
13509	8	2.921799544292874
13509	9	2.188910695431673
13509	10-11	2.0704123029795554
13509	12-13	2.3777245767786397
13509	14-15	3.3409558853486487
13509	16-17	-12.783125191123531
13509	18-19	2.007309905770505
13509	20-21	2.4290827548240443
13509	22-23	2.601560686942001
13509	24-25	-6.9325390595517185
13509	26-27	-0.8369662993745308
13509	28-29	-5.405965789819646
13509	30-31	5.337940070480467
13509	32-33	2.2754863129040217
13509	34-35	7.326933097338564
13509	36-37	7.850886758195372
13509	38-39	7.647411337490894
13509	40-41	-1.3409699638718706
13509	42-43	-14.446056121139833
13509	44-45	-3.427051036667592
13509	46-47	-0.9274141742530944
13509	48-49	6.106803247330745
13509	50-51	-2.846484627135979
13509	52-53	2.706062414723668
13509	54-55	-4.694300616024961
13509	56-57	-2.9094600888749866
13509	58-59	0.06791596121117749
13509	60-61	-2.2078630886101074
13509	62-63	-4.86838677677099
13509	64-65	5.904246266489633
13509	66-67	7.67457885153377
13509	68-69	-0.7123434054613362
13509	70-71	0.6995794571659637
13509	72-73	-0.3461643531625107
13509	74-75	-2.249716496082062
13509	76	-18.896593489373302
22408	1	0.8899649198802599
22408	2	0.7828696317946537
22408	3	1.735149257208242
22408	4	1.4982260924277817
22408	5	1.5622441538892247
22408	6	-2.703397810428399
22408	7	2.8425834429251964
22408	8	-1.078200455707126
22408	9	2.188910695431673
22408	10-11	0.07041230297955536
22408	12-13	0.3777245767786397
22408	14-15	3.3409558853486487
22408	16-17	-8.283125191123531
22408	18-19	2.007309905770505
22408	20-21	2.4290827548240443
22408	22-23	-8.398439313057999
22408	24-25	2.0674609404482815
22408	26-27	-7.336966299374531
22408	28-29	5.594034210180354
22408	30-31	-7.662059929519533
22408	32-33	4.275486312904022
22408	34-35	-8.173066902661436
22408	36-37	-7.649113241804628
22408	38-39	-14.352588662509106
22408	40-41	-5.840969963871871
22408	42-43	1.0539438788601672
22408	44-45	-7.927051036667592
22408	46-47	-2.9274141742530944
22408	48-49	-4.893196752669255
22408	50-51	8.153515372864021
22408	52-53	5.206062414723668
22408	54-55	4.305699383975039
22408	56-57	-7.409460088874987
22408	58-59	-3.4320840387888225
22408	60-61	4.792136911389893
22408	62-63	4.13161322322901
22408	64-65	-3.0957537335103673
22408	66-67	-3.32542114846623
22408	68-69	-2.7123434054613362
22408	70-71	-1.3004205428340363
22408	72-73	4.153835646837489
22408	74-75	-2.249716496082062
22408	76	-4.896593489373302
22409	1	0.8899649198802599
22409	2	0.7828696317946537
22409	3	1.735149257208242
22409	4	1.4982260924277817
22409	5	1.5622441538892247
22409	6	1.2966021895716011
22409	7	-1.1574165570748036
22409	8	2.921799544292874
22409	9	2.188910695431673
22409	10-11	2.0704123029795554
22409	12-13	0.3777245767786397
22409	14-15	3.3409558853486487
22409	16-17	2.7168748088764687
22409	18-19	0.007309905770505054
22409	20-21	2.4290827548240443
22409	22-23	2.601560686942001
22409	24-25	4.0674609404482815
22409	26-27	1.6630337006254692
22409	28-29	5.594034210180354
22409	30-31	5.337940070480467
22409	32-33	-6.724513687095978
22409	34-35	5.326933097338564
22409	36-37	5.850886758195372
22409	38-39	-1.3525886625091061
22409	40-41	-5.840969963871871
22409	42-43	3.553943878860167
22409	44-45	7.572948963332408
22409	46-47	-5.427414174253094
22409	48-49	4.106803247330745
22409	50-51	-7.346484627135979
22409	52-53	-6.293937585276332
22409	54-55	-4.694300616024961
22409	56-57	8.090539911125013
22409	58-59	5.0679159612111775
22409	60-61	8.792136911389893
22409	62-63	4.13161322322901
22409	64-65	3.4042462664896327
22409	66-67	5.67457885153377
22409	68-69	-2.7123434054613362
22409	70-71	5.199579457165964
22409	72-73	6.153835646837489
22409	74-75	2.250283503917938
22409	76	-18.896593489373302
11402	1	0.8899649198802599
11402	2	0.7828696317946537
11402	3	1.735149257208242
11402	4	1.4982260924277817
11402	5	1.5622441538892247
11402	6	1.2966021895716011
11402	7	-19.157416557074804
11402	8	-19.078200455707126
11402	9	2.188910695431673
11402	10-11	-1.9295876970204446
11402	12-13	-8.62227542322136
11402	14-15	3.3409558853486487
11402	16-17	-9.283125191123531
11402	18-19	2.007309905770505
11402	20-21	-8.570917245175956
11402	22-23	-19.398439313058
11402	24-25	-7.9325390595517185
11402	26-27	-7.336966299374531
11402	28-29	-7.405965789819646
11402	30-31	-10.162059929519533
11402	32-33	-5.724513687095978
11402	34-35	-3.6730669026614358
11402	36-37	-4.149113241804628
11402	38-39	-14.352588662509106
11402	40-41	-3.3409699638718706
11402	42-43	5.553943878860167
11402	44-45	-14.427051036667592
11402	46-47	-0.9274141742530944
11402	48-49	-4.893196752669255
11402	50-51	4.153515372864021
11402	52-53	7.206062414723668
11402	54-55	4.305699383975039
11402	56-57	8.090539911125013
11402	58-59	-12.432084038788823
11402	60-61	-6.707863088610107
11402	62-63	4.13161322322901
11402	64-65	-3.0957537335103673
11402	66-67	5.67457885153377
11402	68-69	-2.7123434054613362
11402	70-71	-3.8004205428340363
11402	72-73	-2.3461643531625107
11402	74-75	-8.749716496082062
11402	76	-18.896593489373302
22411	1	-0.09704806713272873
22411	2	-0.2647494158243937
22411	3	0.30657782863681504
22411	4	0.15623475043643964
22411	5	0.28518787683294633
22411	6	-0.3224454294760193
22411	7	1.8512414515832063
22411	8	0.9391155616088938
22411	9	0.6564431629641376
22411	10-11	-0.17201193944468685
22411	12-13	-0.09846589941184192
22411	14-15	0.4383584827512479
22411	16-17	-0.08831999631833298
22411	18-19	-0.7610883626277669
22411	20-21	-1.0600947343534486
22411	22-23	-0.6538505684692595
22411	24-25	0.24711462010196428
22411	26-27	-0.32181478422301524
22411	28-29	0.2390558552019968
22411	30-31	-0.17504694250654396
22411	32-33	0.7343607717784799
22411	34-35	2.1667599371654056
22411	36-37	2.270800178108793
22411	38-39	-0.952155762076206
22411	40-41	0.953402330500424
22411	42-43	-0.9244110994948116
22411	44-45	1.1054164957999397
22411	46-47	3.0595988127598943
22411	48-49	-1.5468764063489076
22411	50-51	1.9716971910458412
22411	52-53	2.935499644160899
22411	54-55	0.4983400766157331
22411	56-57	2.893570214155318
22411	58-59	3.513803407098621
22411	60-61	2.393868513121493
22411	62-63	-0.22120062958484255
22411	64-65	1.326324188567554
22411	66-67	0.8022844792393968
22411	68-69	1.9615225836220453
22411	70-71	2.162366622535245
22411	72-73	0.5652640541001865
22411	74-75	1.7913674200018548
22411	76	6.094556953104576
11404	1	-0.636350869593425
11404	2	-0.2697619471527162
11404	3	1.261465046681927
11404	4	0.629805039796203
11404	5	-0.4640716355844603
11404	6	-1.177082020954714
11404	7	0.6583729166094088
11404	8	0.07969428113497656
11404	9	-0.8637208835156969
11404	10-11	0.2940965135058704
11404	12-13	1.5224614188838999
11404	14-15	-0.06693885149346102
11404	16-17	0.2168748088764687
11404	18-19	-0.71637430475581
11404	20-21	-0.24196987675490078
11404	22-23	0.6541922658893711
11404	24-25	1.054303045711439
11404	26-27	1.9130337006254692
11404	28-29	3.5940342101803537
11404	30-31	3.35109796521731
11404	32-33	3.7491705234303367
11404	34-35	2.853248886812249
11404	36-37	5.035097284511167
11404	38-39	3.989516600648784
11404	40-41	4.172187930864972
11404	42-43	3.9486807209654273
11404	44-45	2.7571594896481955
11404	46-47	5.493638457325851
11404	48-49	1.238382194699163
11404	50-51	4.456146951811391
11404	52-53	4.666588730513141
11404	54-55	1.5162256997645152
11404	56-57	3.0379083321776434
11404	58-59	4.83107385594802
11404	60-61	3.97634743770568
11404	62-63	-0.06575519782361994
11404	64-65	2.759509424384369
11404	66-67	1.3587893778495577
11404	68-69	1.7876565945386638
11404	70-71	4.265368930850176
11404	72-73	2.6538356468374893
11404	74-75	4.799093027727462
11404	76	7.137889269247388
11406	1	-0.9100350801197408
11406	2	-1.883797034872014
11406	3	0.9684825905415764
11406	4	-0.5684405742388847
11406	5	0.9622441538892232
11406	6	-0.10339781042839746
11406	7	0.07591677625853066
11406	8	0.9217995442928739
11406	9	1.0555773620983402
11406	10-11	0.32041230297955536
11406	12-13	-1.772275423221359
11406	14-15	0.10762255201531445
11406	16-17	-0.49979185779019986
11406	18-19	-1.9426900942294978
11406	20-21	0.9957494214907072
11406	22-23	0.7515606869419997
11406	24-25	-1.5492057262183856
11406	26-27	-0.05363296604119938
11406	28-29	2.3107008768470223
11406	30-31	0.7546067371471317
11406	32-33	1.2421529795706903
11406	34-35	1.1935997640052314
11406	36-37	2.2008867581953737
11406	38-39	3.2474113374908953
11406	40-41	5.309030036128128
11406	42-43	1.6539438788601686
11406	44-45	2.4229489633324093
11406	46-47	3.689252492413573
11406	48-49	-1.2598634193359217
11406	50-51	3.6868487061973525
11406	52-53	3.706062414723668
11406	54-55	1.222366050641707
11406	56-57	0.8238732444583476
11406	58-59	3.9512492945445103
11406	60-61	2.742136911389892
11406	62-63	-0.051720110104323425
11406	64-65	-2.2790870668436973
11406	66-67	-0.008754481799563507
11406	68-69	0.5543232612053295
11406	70-71	3.5995794571659623
11406	72-73	1.987168980170825
11406	74-75	4.729210706983071
11406	76	7.456347687097285
23503	1	0.8899649198802599
23503	2	0.7828696317946537
23503	3	1.735149257208242
23503	4	1.4982260924277817
23503	5	1.5622441538892247
23503	6	1.2966021895716011
23503	7	-1.1574165570748036
23503	8	2.921799544292874
23503	9	2.188910695431673
23503	10-11	2.0704123029795554
23503	12-13	0.3777245767786397
23503	14-15	3.3409558853486487
23503	16-17	2.7168748088764687
23503	18-19	2.007309905770505
23503	20-21	2.4290827548240443
23503	22-23	2.601560686942001
23503	24-25	-6.9325390595517185
23503	26-27	1.6630337006254692
23503	28-29	3.5940342101803537
23503	30-31	0.8379400704804674
23503	32-33	6.275486312904022
23503	34-35	-14.673066902661436
23503	36-37	0.3508867581953723
23503	38-39	1.1474113374908939
23503	40-41	-1.3409699638718706
23503	42-43	-3.446056121139833
23503	44-45	-1.4270510366675921
23503	46-47	-5.427414174253094
23503	48-49	6.106803247330745
23503	50-51	8.153515372864021
23503	52-53	-6.293937585276332
23503	54-55	-1.194300616024961
23503	56-57	-13.909460088874987
23503	58-59	-1.4320840387888225
23503	60-61	-2.2078630886101074
23503	62-63	-6.86838677677099
23503	64-65	-3.0957537335103673
23503	66-67	-1.32542114846623
23503	68-69	-5.212343405461336
23503	70-71	-0.3004205428340363
23503	72-73	4.153835646837489
23503	74-75	-2.249716496082062
23503	76	13.103406510626698
11408	1	0.8899649198802599
11408	2	0.7828696317946537
11408	3	1.735149257208242
11408	4	1.4982260924277817
11408	5	1.5622441538892247
11408	6	1.2966021895716011
11408	7	2.8425834429251964
11408	8	2.921799544292874
11408	9	2.188910695431673
11408	10-11	2.0704123029795554
11408	12-13	2.3777245767786397
11408	14-15	-7.659044114651351
11408	16-17	2.7168748088764687
11408	18-19	2.007309905770505
11408	20-21	2.4290827548240443
11408	22-23	0.6015606869420012
11408	24-25	-0.43253905955171845
11408	26-27	3.663033700625469
11408	28-29	3.5940342101803537
11408	30-31	1.3379400704804674
11408	32-33	6.275486312904022
11408	34-35	7.326933097338564
11408	36-37	-3.1491132418046277
11408	38-39	-3.352588662509106
11408	40-41	9.65903003612813
11408	42-43	-3.446056121139833
11408	44-45	-14.427051036667592
11408	46-47	-11.927414174253094
11408	48-49	4.106803247330745
11408	50-51	-2.846484627135979
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12410	18-19	2.007309905770505
12410	20-21	2.4290827548240443
12410	22-23	2.601560686942001
12410	24-25	-6.9325390595517185
12410	26-27	-3.836966299374531
12410	28-29	3.5940342101803537
12410	30-31	-10.162059929519533
12410	32-33	4.275486312904022
12410	34-35	-3.6730669026614358
12410	36-37	-14.149113241804628
12410	38-39	-3.352588662509106
12410	40-41	-12.34096996387187
12410	42-43	5.553943878860167
12410	44-45	7.572948963332408
12410	46-47	-11.927414174253094
12410	48-49	-0.3931967526692546
12410	50-51	-2.846484627135979
12410	52-53	7.206062414723668
12410	54-55	4.305699383975039
12410	56-57	-2.9094600888749866
12410	58-59	-1.4320840387888225
12410	60-61	-2.2078630886101074
12410	62-63	-4.86838677677099
12410	64-65	-3.0957537335103673
12410	66-67	-7.82542114846623
12410	68-69	-0.7123434054613362
12410	70-71	-10.300420542834036
12410	72-73	-2.3461643531625107
12410	74-75	1.250283503917938
12410	76	8.103406510626698
12411	1	-0.2879196955043568
12411	2	-0.04886113743611631
12411	3	1.0115915649005487
12411	4	0.8732260924277817
12411	5	0.7088787692738414
12411	6	-0.03512857965916538
12411	7	1.1743142121559629
12411	8	1.5516072366005673
12411	9	1.4196799262009066
12411	10-11	0.6293065337487818
12411	12-13	0.7250803460094133
12411	14-15	1.3770135776563421
12411	16-17	0.4548555781072352
12411	18-19	-0.8376420173064147
12411	20-21	-0.21875378363749576
12411	22-23	0.35636837924969456
12411	24-25	1.9977494019867486
12411	26-27	1.289235623702389
12411	28-29	2.7454765178726603
12411	30-31	0.7261612243266207
12411	32-33	0.226207466750175
12411	34-35	2.4891927127231774
12411	36-37	3.1369444505030657
12411	38-39	1.2964497990293538
12411	40-41	2.501578113051206
12411	42-43	1.1921650327063205
12411	44-45	1.1823239633324079
12411	46-47	1.5088839026699823
12411	48-49	-0.5061775219000246
12411	50-51	2.6426980651717145
12411	52-53	3.947648953185208
12411	54-55	1.1037763070519624
12411	56-57	3.0412610649711667
12411	58-59	4.1460409612111775
12411	60-61	2.680358065236046
12411	62-63	0.0833888487334491
12411	64-65	1.0846336756906005
12411	66-67	0.5446392666822213
12411	68-69	2.0479972271420728
12411	70-71	1.9747708404666575
12411	72-73	0.4102010460449783
12411	74-75	1.937033503917938
12411	76	6.208934148817654
12412	1	0.8899649198802599
12412	2	0.7828696317946537
12412	3	1.735149257208242
12412	4	1.4982260924277817
12412	5	1.5622441538892247
12412	6	1.2966021895716011
12412	7	2.8425834429251964
12412	8	2.921799544292874
12412	9	2.188910695431673
12412	10-11	0.07041230297955536
12412	12-13	2.3777245767786397
12412	14-15	3.3409558853486487
12412	16-17	0.7168748088764687
12412	18-19	2.007309905770505
12412	20-21	2.4290827548240443
12412	22-23	2.601560686942001
12412	24-25	4.0674609404482815
12412	26-27	3.663033700625469
12412	28-29	1.0940342101803537
12412	30-31	-5.662059929519533
12412	32-33	6.275486312904022
12412	34-35	5.326933097338564
12412	36-37	7.850886758195372
12412	38-39	7.647411337490894
12412	40-41	5.659030036128129
12412	42-43	7.553943878860167
12412	44-45	7.572948963332408
12412	46-47	-0.9274141742530944
12412	48-49	-1.3931967526692546
12412	50-51	8.153515372864021
12412	52-53	4.706062414723668
12412	54-55	6.305699383975039
12412	56-57	8.090539911125013
12412	58-59	5.0679159612111775
12412	60-61	-4.207863088610107
12412	62-63	6.13161322322901
12412	64-65	7.904246266489633
12412	66-67	3.17457885153377
12412	68-69	-0.7123434054613362
12412	70-71	5.199579457165964
12412	72-73	-11.34616435316251
12412	74-75	11.250283503917938
12412	76	-4.896593489373302
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	0.0
19	3.0
20	7.0
21	29.0
22	61.0
23	149.0
24	219.0
25	368.0
26	569.0
27	950.0
28	1626.0
29	2308.0
30	3231.0
31	3809.0
32	3683.0
33	2580.0
34	1035.0
35	67.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.89068767215967	24.43821582177548	28.816507997873675	12.854588508191176
2	35.67045179995168	21.584923894660545	24.368204880405894	18.37641942498188
3	38.00434887654023	19.946847064508336	24.06378352259	17.98502053636144
4	37.95119594104856	20.76830152210679	22.643150519449144	18.637352017395507
5	36.274462430538776	21.092051220101474	22.319400821454458	20.31408552790529
6	35.86236891702509	19.66848692794665	22.577683274537282	21.89146088049099
7	33.756946122251755	18.386083595071273	24.141096883305146	23.71587339937183
8	31.474411636785387	16.135891364229447	27.09611946068719	25.293577538297974
9	30.814206330031407	16.29862285576226	27.378593863251993	25.508576950954335
10-11	29.551330063543453	20.099543356930585	27.101403754620794	23.247722824905168
12-13	27.05001208021261	25.86373520173955	28.69050495288717	18.395747765160667
14-15	22.585165498912783	27.559797052428124	30.891519690746556	18.96351775791254
16-17	23.947813481517276	27.516308287025854	28.518965933800434	20.01691229765644
18-19	24.779530793205925	26.9371088936674	27.193215588682985	21.090144724443693
20-21	27.18179182371702	24.289649173673528	29.073644534647723	19.45491446796173
22-23	28.151351873776793	23.881895281126926	27.892816584917966	20.073936260178314
24-25	25.781665297443578	27.04054511187358	25.189677668776884	21.988111921905958
26-27	22.486408118883652	26.949377793886676	25.098465627642863	25.465748459586806
28-29	23.151280811986467	24.82841952634123	25.17641372643789	26.84388593523441
30-31	24.77403451109285	24.17709894146648	27.338199042969695	23.710667504470976
32-33	24.91420561651119	28.080139204408137	27.376866934119583	19.62878824496109
34-35	23.14273285320702	31.700420513316256	28.348397699260474	16.80844893421625
36-37	21.06675044709749	29.387597273913673	32.57528155058244	16.9703707284064
38-39	20.762240804292137	32.48586205229832	29.65585576876601	17.09604137464353
40-41	23.599497317415054	33.13354922905892	24.097346415969838	19.169607037556187
42-43	22.846577726218097	32.83787703016242	24.671307037896366	19.644238205723124
44-45	20.31710736211147	33.832841881374776	28.307632812877653	17.5424179436361
46-47	19.869966645719533	34.08179049644705	29.301010296321362	16.74723256151206
48-49	22.55522792091652	32.71861555566298	27.365011843186544	17.361144680233963
50-51	21.80596509885435	33.78450234446754	25.259825010876398	19.14970754580171
52-53	23.168402987600007	33.92472988325155	22.49160039641295	20.41526673273549
54-55	26.86588815550881	32.26227605715529	21.72578032446024	19.146055462875655
56-57	27.372086275268405	33.813715059483506	22.163652190734112	16.650546474513977
58-59	26.26321263575454	37.01231163679462	20.905594659313547	15.81888106813729
60-61	22.481182990875865	40.860136982986035	21.009704978339265	15.648975047798833
62-63	20.19335610961692	43.3791282014005	21.087446390928257	15.340069298054324
64-65	19.736106723689645	44.61643136587855	20.508832891891235	15.138629018540566
66-67	20.330514051722037	44.76564213810418	20.050210836766034	14.853632973407757
68-69	20.61921169024754	45.03824255308751	19.6075556533001	14.734990103364856
70-71	20.621233648522853	45.69349860369409	19.876537161334575	13.808730586448483
72-73	20.3208029915371	45.864495178114545	19.80171226136587	14.012989568982483
74-75	20.85232332757085	43.87605427209388	20.692545445020695	14.57907695531458
76	30.18643395477985	0.0	38.823879412931376	30.989686632288777
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	1.5
4	2.0
5	2.0
6	1.5
7	1.5
8	2.5
9	6.0
10	9.5
11	13.5
12	19.0
13	21.5
14	28.5
15	44.5
16	49.5
17	61.5
18	99.5
19	138.5
20	159.0
21	183.0
22	226.0
23	282.0
24	347.5
25	398.5
26	445.5
27	497.5
28	532.0
29	559.5
30	579.0
31	598.5
32	612.5
33	597.0
34	632.0
35	724.0
36	803.0
37	806.5
38	721.5
39	720.5
40	787.5
41	1036.5
42	1392.0
43	1281.0
44	1037.5
45	845.5
46	622.5
47	518.0
48	465.0
49	456.0
50	432.5
51	428.5
52	446.0
53	459.5
54	464.0
55	434.5
56	409.0
57	416.0
58	418.0
59	411.5
60	426.0
61	439.0
62	426.5
63	404.0
64	336.5
65	280.0
66	230.5
67	188.0
68	170.0
69	139.5
70	108.5
71	94.0
72	89.5
73	86.0
74	86.0
75	83.5
76	69.0
77	41.5
78	23.0
79	19.0
80	22.0
81	23.5
82	17.0
83	12.5
84	12.0
85	8.0
86	2.5
87	1.0
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	1.0
94	0.5
95	1.0
96	2.0
97	2.5
98	3.5
99	9.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009664170089393572
2	0.0
3	0.0
4	0.0
5	0.0
6	0.009664170089393572
7	0.0
8	0.009664170089393572
9	0.0
10-11	0.002416042522348393
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.002416042522348393
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1.0
20-21	0.0
22-23	1.0
24-25	0.0
26-27	3.0
28-29	1.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	2.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	6.0
54-55	1.0
56-57	6.0
58-59	14.0
60-61	17.0
62-63	47.0
64-65	60.0
66-67	61.0
68-69	49.0
70-71	67.0
72-73	542.0
74-75	9733.0
76-77	10084.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.2251260655333
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.09038596831957	88.57082699711239
2	1.7646423166680445	3.2195943559723417
3	0.44997334106980524	1.2314662429566663
4	0.17963775259881257	0.6554990650782304
5	0.13042984443584188	0.5949239500683786
6	0.07149571211296701	0.39133232103902954
7	0.06484529478203921	0.4140864133873737
8	0.021395796926839038	0.1561467417538753
9	0.03190343100136255	0.26193550635201085
>10	0.1741998584303379	2.9553681847597706
>50	0.01581801274079311	0.9427601348382414
>100	0.005272670913597695	0.6060600866817258
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	126	0.6088427156317952	No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAAA	68	0.32858178303938146	No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	65	0.31408552790529115	No Hit
AGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	63	0.3044213578158976	No Hit
TCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	40	0.19328340178787146	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAAAAAAAAAAAAAA	37	0.17878714665378112	No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	32	0.15462672143029715	No Hit
GGGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTG	32	0.15462672143029715	No Hit
GGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGC	29	0.14013046629620682	No Hit
GCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	28	0.13529838125151003	No Hit
GTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGCA	27	0.13046629620681324	No Hit
AAGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	27	0.13046629620681324	No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	24	0.11597004107272288	No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAAAAAAAAAAAAAAA	23	0.1111379560280261	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10	0.0	0.0	0.0
11	0.0	0.0	0.0
12	0.0	0.0	0.0
13	0.0	0.0	0.0
14	0.0	0.0	0.0
15	0.0	0.0	0.0
16	0.0	0.0	0.0
17	0.0	0.0	0.0
18	0.0	0.0	0.0
19	0.0	0.0	0.0
20	0.0	0.0	0.0
21	0.0	0.0	0.0
22	0.0	0.0	0.0
23	0.0	0.0	0.0
24	0.0	0.0	0.0
25	0.0	0.0	0.0
26	0.004832085044696787	0.0	0.0
27	0.004832085044696787	0.0	0.0
28	0.024160425223483932	0.0	0.0
29	0.048320850446967864	0.0	0.0
30	0.06764919062575502	0.0	0.0
31	0.08697753080454217	0.0	0.0
32	0.10147378593863252	0.0	0.0
33	0.12563421116211645	0.0	0.0
34	0.14013046629620682	0.0	0.0
35	0.15462672143029718	0.0	0.0
36	0.16429089151969076	0.0	0.0
37	0.19811548683256824	0.0	0.0
38	0.20294757187726503	0.0	0.0
39	0.2126117419666586	0.0	0.0
40	0.2174438270113554	0.0	0.0
41	0.23677216719014255	0.0	0.0
42	0.2512684223242329	0.0	0.0
43	0.27059676250302006	0.0	0.0
44	0.2899251026818072	0.0	0.0
45	0.3237496979946847	0.0	0.0
46	0.3285817830393815	0.0	0.0
47	0.3334138680840783	0.0	0.0
48	0.36723846339695576	0.0	0.0
49	0.3817347185310461	0.0	0.0
50	0.4348876540227108	0.0	0.0
51	0.49770475960376903	0.0	0.0
52	0.5363614399613433	0.0	0.0
53	0.5846822904083112	0.0	0.0
54	0.6523314810340662	0.0	0.0
55	0.6861560763469438	0.0	0.0
56	0.7296448417492147	0.0	0.0
57	0.8021261174196666	0.0	0.0
58	0.8552790529113312	0.0	0.0
59	0.8987678183136023	0.0	0.0
60	0.9325924136264798	0.0	0.0
61	0.9664170089393573	0.0	0.0
62	0.9809132640734477	0.0	0.0
63	0.9809132640734477	0.0	0.0
64	0.9809132640734477	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGAAGG	30	0.0021858225	69.80975	70
ATGTCCT	35	0.0040268498	59.836937	70
CTGAGTT	20	0.0071331356	51.977	31
GCCTCAG	20	0.0071331356	51.977	7
AAAACTC	20	0.0071331356	51.977	22
GGGGGGG	95	0.005431852	29.393583	70
GAGGAGA	60	5.10972E-4	28.87611	57
AGCGACC	60	5.10972E-4	28.87611	4
AGGAGAA	80	0.0027347873	21.657084	58
CGCGACC	100	4.019066E-4	20.7908	3
GGAGAAA	85	0.0038325544	20.43261	59
GGAGGAG	90	0.005411194	19.25074	56
CGACCTC	200	2.3730536E-8	19.058233	5
CTCAGAT	250	8.1308826E-10	18.018692	9
GTGGCGA	250	8.1308826E-10	18.018692	21
TGGCGAC	250	8.1308826E-10	18.018692	22
GGCGACC	255	1.0586518E-9	17.665384	23
CGTGGCG	255	1.0586518E-9	17.665384	20
TCAGATC	255	1.0586518E-9	17.665384	10
AGATCAG	255	1.0586518E-9	17.665384	12
>>END_MODULE
