OU_LexBC003_S43_L004_R1_001.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	OU_LexBC003_S43_L004_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20695
Sequences flagged as poor quality	0
Sequence length	19-76
%GC	43

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	126	0.6088427156317952	No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAAA	68	0.32858178303938146	No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	65	0.31408552790529115	No Hit
AGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	63	0.3044213578158976	No Hit
TCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	40	0.19328340178787146	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAAAAAAAAAAAAAA	37	0.17878714665378112	No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	32	0.15462672143029715	No Hit
GGGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTG	32	0.15462672143029715	No Hit
GGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGC	29	0.14013046629620682	No Hit
GCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	28	0.13529838125151003	No Hit
GTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGCA	27	0.13046629620681324	No Hit
AAGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	27	0.13046629620681324	No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	24	0.11597004107272288	No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAAAAAAAAAAAAAAA	23	0.1111379560280261	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGAAGG	30	0.0021858225	69.80975	70
ATGTCCT	35	0.0040268498	59.836937	70
CTGAGTT	20	0.0071331356	51.977	31
GCCTCAG	20	0.0071331356	51.977	7
AAAACTC	20	0.0071331356	51.977	22
GGGGGGG	95	0.005431852	29.393583	70
GAGGAGA	60	5.10972E-4	28.87611	57
AGCGACC	60	5.10972E-4	28.87611	4
AGGAGAA	80	0.0027347873	21.657084	58
CGCGACC	100	4.019066E-4	20.7908	3
GGAGAAA	85	0.0038325544	20.43261	59
GGAGGAG	90	0.005411194	19.25074	56
CGACCTC	200	2.3730536E-8	19.058233	5
CTCAGAT	250	8.1308826E-10	18.018692	9
GTGGCGA	250	8.1308826E-10	18.018692	21
TGGCGAC	250	8.1308826E-10	18.018692	22
GGCGACC	255	1.0586518E-9	17.665384	23
CGTGGCG	255	1.0586518E-9	17.665384	20
TCAGATC	255	1.0586518E-9	17.665384	10
AGATCAG	255	1.0586518E-9	17.665384	12