FastQCFastQC Report
Wed 15 Feb 2017
OU_LexBC003_S43_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOU_LexBC003_S43_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23245
Sequences flagged as poor quality0
Sequence length42-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA1990.8560980856098086No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAAA780.3355560335556034No Hit
AGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA580.24951602495160247No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA540.2323080232308023No Hit
TCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA500.21510002151000218No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAAAAAAAAAAAAAA470.202194020219402No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA460.19789201978920198No Hit
GGGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTG350.1505700150570015No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA320.1376640137664014No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA290.12475801247580123No Hit
GTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGCA280.1204560120456012No Hit
GGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGC280.1204560120456012No Hit
AAGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA260.11185201118520112No Hit
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA250.10755001075500109No Hit
ATGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA250.10755001075500109No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA200.007061375352.12986860
AGGGGGC200.007183421451.905171
TCAGAGA200.007183421451.9051744
GCGGAGA306.0285244E-446.2375852
CGCGACC1450.033.4102251
CGGAGAA450.00441792930.8250553
AGCGACC605.1708234E-428.8362064
CGACCTC3150.025.266013
GCGACCT2800.023.4809112
ACCTCAG3400.023.4082155
GACCTCA3400.023.4082154
AGCGGAG3900.023.11879251
GCGACCC3900.023.06896622
GGCGACC3900.023.06896621
ACCCGCT3900.023.06896625
CGACCCG3900.023.06896623
CGCTGAA3950.022.77695328
CCGCTGA3950.022.77695327
CCCGCTG3950.022.77695326
CGTGGCG3950.022.77695318
Produced by FastQC (version 0.11.2)