##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	OU_LexBC003_S43_L001_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21045
Sequences flagged as poor quality	0
Sequence length	23-76
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.125112853409362	32.0	32.0	32.0	32.0	32.0
2	31.076217628890472	32.0	32.0	32.0	32.0	32.0
3	31.325635542884296	32.0	32.0	32.0	32.0	32.0
4	31.302304585412212	32.0	32.0	32.0	32.0	32.0
5	31.198526966025184	32.0	32.0	32.0	32.0	32.0
6	34.67693038726539	36.0	36.0	36.0	32.0	36.0
7	34.58232359230221	36.0	36.0	36.0	32.0	36.0
8	34.534616298408174	36.0	36.0	36.0	32.0	36.0
9	34.45065336184367	36.0	36.0	36.0	32.0	36.0
10-11	34.364385839866955	36.0	36.0	36.0	32.0	36.0
12-13	33.98142076502732	36.0	36.0	36.0	32.0	36.0
14-15	33.538679021145164	36.0	36.0	36.0	27.0	36.0
16-17	33.5455690187693	36.0	36.0	36.0	27.0	36.0
18-19	33.50408648134949	36.0	36.0	36.0	27.0	36.0
20-21	33.13882157282015	36.0	32.0	36.0	27.0	36.0
22-23	33.057472083630316	36.0	32.0	36.0	27.0	36.0
24-25	32.80367325603497	36.0	32.0	36.0	27.0	36.0
26-27	32.38433757840715	36.0	32.0	36.0	20.5	36.0
28-29	32.19732465310777	36.0	32.0	36.0	17.5	36.0
30-31	31.212911043527846	34.0	29.5	36.0	14.0	36.0
32-33	30.749714883102072	34.0	29.5	36.0	14.0	36.0
34-35	31.737074700627257	36.0	32.0	36.0	14.0	36.0
36-37	30.779747196350506	34.0	29.5	36.0	14.0	36.0
38-39	28.537797180361032	32.0	24.0	34.0	14.0	36.0
40-41	28.298664575610687	32.0	24.0	36.0	14.0	36.0
42-43	28.78115198175078	32.0	27.0	36.0	14.0	36.0
44-45	28.55439121756487	32.0	27.0	36.0	14.0	36.0
46-47	28.801311165465556	32.0	27.0	36.0	14.0	36.0
48-49	29.72797053231939	32.0	27.0	36.0	14.0	36.0
50-51	29.775332699619774	32.0	27.0	36.0	14.0	36.0
52-53	30.38667035198303	34.0	27.0	36.0	14.0	36.0
54-55	31.290925943530752	36.0	32.0	36.0	14.0	36.0
56-57	31.428397090151254	36.0	32.0	36.0	14.0	36.0
58-59	30.784677700261255	36.0	27.0	36.0	14.0	36.0
60-61	29.713104737071923	32.0	27.0	36.0	14.0	36.0
62-63	29.514785457212284	32.0	27.0	36.0	14.0	36.0
64-65	29.99997818765771	34.0	27.0	36.0	14.0	36.0
66-67	29.08679712529902	32.0	27.0	36.0	14.0	36.0
68-69	27.916597616412275	32.0	24.0	36.0	14.0	36.0
70-71	27.13145045443408	32.0	21.0	34.0	14.0	36.0
72-73	25.58182665680176	27.0	14.0	32.0	14.0	36.0
74-75	24.960042121569266	27.0	14.0	32.0	14.0	34.0
76	25.567477646080267	27.0	14.0	32.0	14.0	36.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
21312	1	1.3552815412714345
21312	2	1.6624425653388109
21312	3	1.637119134844344
21312	4	0.1675440034334983
21312	5	0.04173264179063452
21312	6	1.2562928950631118
21312	7	2.34830108159224
21312	8	0.02406055510745375
21312	9	0.7478413243142157
21312	10-11	0.2817909967402201
21312	12-13	-0.26732199814997415
21312	14-15	-0.38746768645449947
21312	16-17	-0.16089439386640692
21312	18-19	0.5426431260546707
21312	20-21	-0.31679659807749516
21312	22-23	-1.305992352872309
21312	24-25	0.005522540588060565
21312	26-27	1.1233184817146693
21312	28-29	-0.6786002828922406
21312	30-31	1.0687994682868762
21312	32-33	-0.6464352317260271
21312	34-35	-0.021945669548365743
21312	36-37	1.4148463829281184
21312	38-39	-0.33663453411450917
21312	40-41	1.0998875868636908
21312	42-43	0.5743350565107548
21312	44-45	-0.3039771494379906
21312	46-47	-1.349350514323401
21312	48-49	0.6866946983803288
21312	50-51	1.8820373654254574
21312	52-53	2.0165684866050135
21312	54-55	1.4613246412836993
21312	56-57	1.435472464245123
21312	58-59	1.2559652937606351
21312	60-61	1.034237238985959
21312	62-63	2.0838240516364124
21312	64-65	3.7273987538749793
21312	66-67	1.533643869812611
21312	68-69	3.2954476335945166
21312	70-71	-0.4990797997545222
21312	72-73	-0.2765836500484582
21312	74-75	1.7701447577566114
21312	76	9.917599095924666
12101	1	2.205281541271436
12101	2	1.308871136767383
12101	3	-0.4597062619810508
12101	4	-0.7614242505347555
12101	5	-0.06541021535222313
12101	6	-1.151643612873393
12101	7	2.6490947323858904
12101	8	-0.4707807147338201
12101	9	0.134746086218982
12101	10-11	0.470679885629103
12101	12-13	1.3894240335960504
12101	14-15	1.1815799325931238
12101	16-17	0.23275639978439244
12101	18-19	0.6321272530388029
12101	20-21	-1.732868026648923
12101	22-23	-1.6409129877929445
12101	24-25	0.25988761995314036
12101	26-27	1.274508957905148
12101	28-29	1.1829076536157004
12101	30-31	-0.33993069044328195
12101	32-33	-0.3377050729958704
12101	34-35	0.26932417172147893
12101	36-37	-1.879598061516326
12101	38-39	-2.37383371486208
12101	40-41	-1.7433023772940182
12101	42-43	0.48024903500537874
12101	44-45	-1.1220775078609293
12101	46-47	-1.0545476469398878
12101	48-49	1.1705656661222648
12101	50-51	-0.066887365757335
12101	52-53	1.3131634686838645
12101	54-55	1.5984214154772474
12101	56-57	0.9542896685462026
12101	58-59	1.0875065123986296
12101	60-61	1.975097454039723
12101	62-63	1.2540749476937592
12101	64-65	1.9845672126563407
12101	66-67	2.465543511389672
12101	68-69	0.5956268450640501
12101	70-71	3.2464399135071247
12101	72-73	1.0227533509389843
12101	74-75	2.1611902447773303
12101	76	8.808309478438327
12102	1	2.205281541271436
12102	2	2.308871136767383
12102	3	2.15140484913006
12102	4	0.8496868605763552
12102	5	0.9345897846477769
12102	6	2.5705786093488285
12102	7	3.926872510163669
12102	8	1.584774840821737
12102	9	1.8014127528856463
12102	10-11	2.3317909967402173
12102	12-13	2.0005351447071646
12102	14-15	2.598246599259788
12102	16-17	2.260534177562164
12102	18-19	1.3265716974832458
12102	20-21	0.7671319733510771
12102	22-23	2.386864789984834
12102	24-25	3.537665397730919
12102	26-27	4.191175624571812
12102	28-29	3.210685431393472
12102	30-31	4.104513754001161
12102	32-33	5.217850482559687
12102	34-35	4.963768616165922
12102	36-37	4.314846382928117
12102	38-39	-8.37383371486208
12102	40-41	6.78447540048376
12102	42-43	-6.6586398538835105
12102	44-45	-4.760966396749819
12102	46-47	-4.860103202495445
12102	48-49	2.837232332788929
12102	50-51	7.905334856464886
12102	52-53	-3.659058753538357
12102	54-55	3.9039769710328045
12102	56-57	4.148734112990645
12102	58-59	4.0319509568430725
12102	60-61	2.6973196762619445
12102	62-63	-4.968147274528462
12102	64-65	-12.98765500956588
12102	66-67	4.409987955834115
12102	68-69	1.9567379561751608
12102	70-71	4.9131065801737925
12102	72-73	1.459027860742907
12102	74-75	3.2935431859538014
12102	76	16.474976145104996
11208	1	-15.794718458728564
11208	2	2.308871136767383
11208	3	-15.84859515086994
11208	4	0.8496868605763552
11208	5	0.9345897846477769
11208	6	2.5705786093488285
11208	7	3.926872510163669
11208	8	1.584774840821737
11208	9	1.8014127528856463
11208	10-11	2.3317909967402173
11208	12-13	-16.499464855292835
11208	14-15	-4.901753400740212
11208	16-17	0.2605341775621639
11208	18-19	-7.673428302516754
11208	20-21	-8.232868026648923
11208	22-23	0.3868647899848341
11208	24-25	1.537665397730919
11208	26-27	4.191175624571812
11208	28-29	1.2106854313934718
11208	30-31	-9.395486245998839
11208	32-33	-10.282149517440313
11208	34-35	2.963768616165922
11208	36-37	6.314846382928117
11208	38-39	7.12616628513792
11208	40-41	4.28447540048376
11208	42-43	8.34136014611649
11208	44-45	8.239033603250181
11208	46-47	-5.860103202495445
11208	48-49	5.337232332788929
11208	50-51	-3.0946651435351136
11208	52-53	-3.659058753538357
11208	54-55	-5.0960230289671955
11208	56-57	6.148734112990645
11208	58-59	6.0319509568430725
11208	60-61	7.1973196762619445
11208	62-63	4.031852725471538
11208	64-65	-12.98765500956588
11208	66-67	-4.590012044165885
11208	68-69	-1.5432620438248392
11208	70-71	9.413106580173793
11208	72-73	-0.540972139257093
11208	74-75	-9.706456814046199
11208	76	-15.525023854895005
21201	1	2.205281541271436
21201	2	2.308871136767383
21201	3	2.15140484913006
21201	4	0.8496868605763552
21201	5	0.9345897846477769
21201	6	-12.429421390651171
21201	7	3.926872510163669
21201	8	1.584774840821737
21201	9	1.8014127528856463
21201	10-11	2.3317909967402173
21201	12-13	2.0005351447071646
21201	14-15	2.598246599259788
21201	16-17	2.260534177562164
21201	18-19	3.3265716974832458
21201	20-21	0.7671319733510771
21201	22-23	2.386864789984834
21201	24-25	3.537665397730919
21201	26-27	4.191175624571812
21201	28-29	3.210685431393472
21201	30-31	2.104513754001161
21201	32-33	5.217850482559687
21201	34-35	4.963768616165922
21201	36-37	-1.185153617071883
21201	38-39	3.12616628513792
21201	40-41	2.2844754004837604
21201	42-43	8.34136014611649
21201	44-45	3.7390336032501814
21201	46-47	7.139896797504555
21201	48-49	7.337232332788929
21201	50-51	7.905334856464886
21201	52-53	7.340941246461643
21201	54-55	5.9039769710328045
21201	56-57	6.148734112990645
21201	58-59	6.0319509568430725
21201	60-61	-3.8026803237380555
21201	62-63	-3.9681472745284623
21201	64-65	7.012344990434119
21201	66-67	6.409987955834115
21201	68-69	-1.5432620438248392
21201	70-71	9.413106580173793
21201	72-73	-11.540972139257093
21201	74-75	-9.706456814046199
21201	76	-15.525023854895005
12307	1	-8.794718458728564
12307	2	2.308871136767383
12307	3	2.15140484913006
12307	4	0.8496868605763552
12307	5	0.9345897846477769
12307	6	-1.4294213906511715
12307	7	3.926872510163669
12307	8	1.584774840821737
12307	9	1.8014127528856463
12307	10-11	2.3317909967402173
12307	12-13	2.0005351447071646
12307	14-15	2.598246599259788
12307	16-17	0.2605341775621639
12307	18-19	3.3265716974832458
12307	20-21	2.767131973351077
12307	22-23	2.386864789984834
12307	24-25	3.537665397730919
12307	26-27	4.191175624571812
12307	28-29	3.210685431393472
12307	30-31	4.104513754001161
12307	32-33	3.2178504825596868
12307	34-35	0.4637686161659218
12307	36-37	-0.18515361707188305
12307	38-39	5.12616628513792
12307	40-41	4.78447540048376
12307	42-43	3.8413601461164895
12307	44-45	-0.7609663967498186
12307	46-47	7.139896797504555
12307	48-49	7.337232332788929
12307	50-51	7.905334856464886
12307	52-53	7.340941246461643
12307	54-55	5.9039769710328045
12307	56-57	6.148734112990645
12307	58-59	6.0319509568430725
12307	60-61	7.1973196762619445
12307	62-63	8.031852725471538
12307	64-65	9.01234499043412
12307	66-67	8.409987955834115
12307	68-69	7.456737956175161
12307	70-71	9.413106580173793
12307	72-73	-0.540972139257093
12307	74-75	10.293543185953801
12307	76	-15.525023854895005
12308	1	2.205281541271436
12308	2	-15.691128863232617
12308	3	2.15140484913006
12308	4	0.8496868605763552
12308	5	0.9345897846477769
12308	6	2.5705786093488285
12308	7	3.926872510163669
12308	8	1.584774840821737
12308	9	1.8014127528856463
12308	10-11	2.3317909967402173
12308	12-13	2.0005351447071646
12308	14-15	2.598246599259788
12308	16-17	0.2605341775621639
12308	18-19	3.3265716974832458
12308	20-21	2.767131973351077
12308	22-23	-8.613135210015166
12308	24-25	-7.462334602269081
12308	26-27	-0.30882437542818764
12308	28-29	-0.7893145686065282
12308	30-31	-1.395486245998839
12308	32-33	0.7178504825596868
12308	34-35	0.4637686161659218
12308	36-37	6.314846382928117
12308	38-39	7.12616628513792
12308	40-41	-2.2155245995162396
12308	42-43	-13.65863985388351
12308	44-45	4.239033603250181
12308	46-47	2.639896797504555
12308	48-49	3.337232332788929
12308	50-51	7.905334856464886
12308	52-53	-11.159058753538357
12308	54-55	5.9039769710328045
12308	56-57	-12.351265887009355
12308	58-59	6.0319509568430725
12308	60-61	7.1973196762619445
12308	62-63	-2.9681472745284623
12308	64-65	-3.9876550095658807
12308	66-67	3.909987955834115
12308	68-69	7.456737956175161
12308	70-71	4.9131065801737925
12308	72-73	10.459027860742907
12308	74-75	1.2935431859538014
12308	76	-1.5250238548950055
12309	1	-8.794718458728564
12309	2	-8.691128863232617
12309	3	-8.84859515086994
12309	4	-10.150313139423645
12309	5	0.9345897846477769
12309	6	-12.429421390651171
12309	7	-18.07312748983633
12309	8	1.584774840821737
12309	9	1.8014127528856463
12309	10-11	2.3317909967402173
12309	12-13	2.0005351447071646
12309	14-15	2.598246599259788
12309	16-17	2.260534177562164
12309	18-19	-9.673428302516754
12309	20-21	2.767131973351077
12309	22-23	0.3868647899848341
12309	24-25	-9.462334602269081
12309	26-27	2.1911756245718124
12309	28-29	-1.2893145686065282
12309	30-31	-4.895486245998839
12309	32-33	5.217850482559687
12309	34-35	-2.536231383834078
12309	36-37	-2.685153617071883
12309	38-39	-14.87383371486208
12309	40-41	-6.71552459951624
12309	42-43	-13.65863985388351
12309	44-45	-0.7609663967498186
12309	46-47	-5.860103202495445
12309	48-49	7.337232332788929
12309	50-51	7.905334856464886
12309	52-53	-3.659058753538357
12309	54-55	-7.0960230289671955
12309	56-57	4.148734112990645
12309	58-59	-15.968049043156928
12309	60-61	-0.30268032373805553
12309	62-63	-1.4681472745284623
12309	64-65	-6.487655009565881
12309	66-67	-3.590012044165885
12309	68-69	-1.5432620438248392
12309	70-71	7.4131065801737925
12309	72-73	10.459027860742907
12309	74-75	5.793543185953801
12309	76	-15.525023854895005
22101	1	-8.794718458728564
22101	2	2.308871136767383
22101	3	2.15140484913006
22101	4	0.8496868605763552
22101	5	0.9345897846477769
22101	6	2.5705786093488285
22101	7	3.926872510163669
22101	8	1.584774840821737
22101	9	1.8014127528856463
22101	10-11	2.3317909967402173
22101	12-13	2.0005351447071646
22101	14-15	0.5982465992597881
22101	16-17	2.260534177562164
22101	18-19	3.3265716974832458
22101	20-21	2.767131973351077
22101	22-23	2.386864789984834
22101	24-25	-9.462334602269081
22101	26-27	4.191175624571812
22101	28-29	-4.289314568606528
22101	30-31	4.104513754001161
22101	32-33	3.2178504825596868
22101	34-35	-6.036231383834078
22101	36-37	-6.685153617071883
22101	38-39	-8.37383371486208
22101	40-41	-9.71552459951624
22101	42-43	-13.65863985388351
22101	44-45	-13.760966396749819
22101	46-47	3.139896797504555
22101	48-49	-14.662767667211071
22101	50-51	-7.594665143535114
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21309	1	2.205281541271436
21309	2	2.308871136767383
21309	3	2.15140484913006
21309	4	0.8496868605763552
21309	5	0.9345897846477769
21309	6	-19.42942139065117
21309	7	3.926872510163669
21309	8	1.584774840821737
21309	9	1.8014127528856463
21309	10-11	2.3317909967402173
21309	12-13	-10.999464855292835
21309	14-15	-1.901753400740212
21309	16-17	2.260534177562164
21309	18-19	1.3265716974832458
21309	20-21	2.767131973351077
21309	22-23	-2.113135210015166
21309	24-25	1.537665397730919
21309	26-27	-6.808824375428188
21309	28-29	3.210685431393472
21309	30-31	6.104513754001161
21309	32-33	-5.782149517440313
21309	34-35	4.963768616165922
21309	36-37	1.814846382928117
21309	38-39	3.12616628513792
21309	40-41	6.78447540048376
21309	42-43	-2.6586398538835105
21309	44-45	6.239033603250181
21309	46-47	5.139896797504555
21309	48-49	-5.662767667211071
21309	50-51	-7.594665143535114
21309	52-53	5.340941246461643
21309	54-55	5.9039769710328045
21309	56-57	-6.851265887009355
21309	58-59	-3.4680490431569275
21309	60-61	5.1973196762619445
21309	62-63	-2.9681472745284623
21309	64-65	4.512344990434119
21309	66-67	-2.590012044165885
21309	68-69	-3.5432620438248392
21309	70-71	4.9131065801737925
21309	72-73	5.959027860742907
21309	74-75	-9.706456814046199
21309	76	-15.525023854895005
23101	1	1.377324552024124
23101	2	1.499731351821147
23101	3	1.597641408269844
23101	4	0.22334277455485108
23101	5	0.40501989217465706
23101	6	1.2668151684886126
23101	7	2.1795606822066773
23101	8	0.17886086232711307
23101	9	0.05410092492865459
23101	10-11	0.18931787846064907
23101	12-13	-0.04919603808853168
23101	14-15	0.060612190657636233
23101	16-17	-0.11177765039482779
23101	18-19	0.7674319125370062
23101	20-21	0.06283089808226094
23101	22-23	-0.3550706938861268
23101	24-25	-0.1814206237744571
23101	26-27	0.45058422672234855
23101	28-29	-0.6522177944129766
23101	30-31	1.1797825712054646
23101	32-33	0.8388182244951707
23101	34-35	1.4153815193917296
23101	36-37	1.6858141248636045
23101	38-39	0.544177037826092
23101	40-41	1.4484538951074164
23101	42-43	2.1263063826756294
23101	44-45	1.6893024204544815
23101	46-47	1.2003806684722989
23101	48-49	1.1625011499932327
23101	50-51	2.3811413080777903
23101	52-53	2.722661676569171
23101	54-55	1.4012887989897962
23101	56-57	1.6541104570766691
23101	58-59	1.3666283761979123
23101	60-61	0.6220508590576408
23101	62-63	1.5681430480521854
23101	64-65	3.3627493031025857
23101	66-67	2.179529734809858
23101	68-69	1.2222366084662646
23101	70-71	0.7480342636897568
23101	72-73	-0.2570047479527453
23101	74-75	1.3665481455636694
23101	76	11.862616594543196
21310	1	1.61861487460477
21310	2	1.2955378034340512
21310	3	1.3780715157967265
21310	4	0.5563535272430222
21310	5	0.214589784647778
21310	6	1.410578609348832
21310	7	3.4602058434970004
21310	8	0.2247748408217376
21310	9	0.3880794195523123
21310	10-11	1.3984576634068802
21310	12-13	0.23386847804049893
21310	14-15	0.5849132659264527
21310	16-17	0.10053417756216732
21310	18-19	1.406571697483244
21310	20-21	-0.012868026648924058
21310	22-23	-0.1998018766818319
21310	24-25	0.15099873106425576
21310	26-27	0.5778422912384826
21310	28-29	-0.02931456860652304
21310	30-31	0.7311804206678261
21310	32-33	-0.055482850773646675
21310	34-35	-1.1628980505007434
21310	36-37	1.4081797162614507
21310	38-39	-1.027167048195416
21310	40-41	1.337808733817095
21310	42-43	0.2680268127831589
21310	44-45	2.3657002699168466
21310	46-47	-1.4534365358287786
21310	48-49	-0.3561010005444061
21310	50-51	0.9786681897982206
21310	52-53	-0.7657254202050261
21310	54-55	1.4639769710328068
21310	56-57	1.3487341129906483
21310	58-59	1.011950956843073
21310	60-61	-0.6826803237380581
21310	62-63	-1.5214806078617968
21310	64-65	2.952344990434117
21310	66-67	1.7207987666449256
21310	68-69	0.3283595777967818
21310	70-71	-1.363920446853232
21310	72-73	-0.7707018689868192
21310	74-75	0.5809574659053922
21310	76	8.321129991258841
23102	1	2.205281541271436
23102	2	2.308871136767383
23102	3	2.15140484913006
23102	4	0.8496868605763552
23102	5	0.9345897846477769
23102	6	2.5705786093488285
23102	7	3.926872510163669
23102	8	1.584774840821737
23102	9	-2.1985872471143537
23102	10-11	2.3317909967402173
23102	12-13	-8.999464855292835
23102	14-15	2.598246599259788
23102	16-17	2.260534177562164
23102	18-19	1.3265716974832458
23102	20-21	2.767131973351077
23102	22-23	2.386864789984834
23102	24-25	-0.9623346022690811
23102	26-27	-6.808824375428188
23102	28-29	1.2106854313934718
23102	30-31	4.104513754001161
23102	32-33	5.217850482559687
23102	34-35	2.963768616165922
23102	36-37	-4.685153617071883
23102	38-39	7.12616628513792
23102	40-41	-13.21552459951624
23102	42-43	-4.6586398538835105
23102	44-45	8.239033603250181
23102	46-47	0.6398967975045551
23102	48-49	7.337232332788929
23102	50-51	-14.094665143535114
23102	52-53	-3.659058753538357
23102	54-55	3.9039769710328045
23102	56-57	4.148734112990645
23102	58-59	1.5319509568430725
23102	60-61	-14.802680323738056
23102	62-63	-10.468147274528462
23102	64-65	-3.9876550095658807
23102	66-67	-13.590012044165885
23102	68-69	-1.5432620438248392
23102	70-71	-12.586893419826207
23102	72-73	-5.040972139257093
23102	74-75	-9.706456814046199
23102	76	11.474976145104995
13311	1	1.2504428315940181
13311	2	0.5862904916060927
13311	3	1.4997919459042528
13311	4	-0.1761195910365494
13311	5	0.28297688142196975
13311	6	1.8479979641875417
13311	7	2.430098316615279
13311	8	0.2944522601765769
13311	9	0.6659288819179068
13311	10-11	1.0414684160950571
13311	12-13	0.6715028866426493
13311	14-15	0.10469821216300801
13311	16-17	0.8185986936911931
13311	18-19	1.5556039555477668
13311	20-21	-0.2812551234231151
13311	22-23	-0.6808771454990321
13311	24-25	-0.5494313764626284
13311	26-27	0.604078850378265
13311	28-29	-0.3183468266710463
13311	30-31	2.0077395604527766
13311	32-33	0.3372053212693622
13311	34-35	0.20892990648849974
13311	36-37	1.4245238022829554
13311	38-39	0.2681017690088865
13311	40-41	1.1102818520966657
13311	42-43	0.2542633719229421
13311	44-45	0.5583884419598597
13311	46-47	-0.5471999766889937
13311	48-49	0.9114258811760223
13311	50-51	1.8246896951745661
13311	52-53	1.8764251174293847
13311	54-55	0.7007511645811917
13311	56-57	2.597121209764836
13311	58-59	1.0254993439398454
13311	60-61	1.6650616117458164
13311	62-63	1.58023982224573
13311	64-65	4.064586297514136
13311	66-67	1.793104838950999
13311	68-69	1.1223223717595765
13311	70-71	1.5786909957582083
13311	72-73	0.47880546875580876
13311	74-75	1.4469741235462301
13311	76	11.197198367327216
21311	1	2.205281541271436
21311	2	2.308871136767383
21311	3	2.15140484913006
21311	4	0.8496868605763552
21311	5	0.9345897846477769
21311	6	-1.4294213906511715
21311	7	-0.073127489836331
21311	8	-20.415225159178263
21311	9	1.8014127528856463
21311	10-11	0.3317909967402173
21311	12-13	-8.999464855292835
21311	14-15	-8.401753400740212
21311	16-17	2.260534177562164
21311	18-19	3.3265716974832458
21311	20-21	0.7671319733510771
21311	22-23	0.3868647899848341
21311	24-25	3.537665397730919
21311	26-27	4.191175624571812
21311	28-29	-7.789314568606528
21311	30-31	-4.895486245998839
21311	32-33	-5.782149517440313
21311	34-35	0.9637686161659218
21311	36-37	6.314846382928117
21311	38-39	-1.87383371486208
21311	40-41	6.78447540048376
21311	42-43	3.8413601461164895
21311	44-45	1.7390336032501814
21311	46-47	7.139896797504555
21311	48-49	7.337232332788929
21311	50-51	7.905334856464886
21311	52-53	5.340941246461643
21311	54-55	5.9039769710328045
21311	56-57	6.148734112990645
21311	58-59	1.5319509568430725
21311	60-61	-5.8026803237380555
21311	62-63	3.5318527254715377
21311	64-65	-1.9876550095658807
21311	66-67	8.409987955834115
21311	68-69	4.956737956175161
21311	70-71	4.9131065801737925
21311	72-73	3.959027860742907
21311	74-75	-0.7064568140461986
21311	76	-15.525023854895005
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	5.0
19	9.0
20	14.0
21	35.0
22	100.0
23	174.0
24	262.0
25	398.0
26	617.0
27	956.0
28	1343.0
29	2177.0
30	3373.0
31	4386.0
32	4173.0
33	2455.0
34	561.0
35	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.982702908192362	21.80193879490591	32.13742634480137	15.077931952100363
2	35.17700166310288	20.674744594915655	25.820860061772393	18.327393680209074
3	36.19387027797576	19.657875980042768	25.11285340936089	19.035400332620576
4	37.96151104775481	20.498930862437632	24.03421240199572	17.505345687811833
5	34.93941553813257	21.848420052268946	23.948681397006418	19.263483012592065
6	34.74934663815633	19.55808980755524	23.52577809455928	22.16678545972915
7	32.23093371347113	17.99477310525065	25.535756711808034	24.23853646947018
8	30.805416963649325	15.547636018056545	28.657638393917793	24.989308624376335
9	29.48918983131385	16.692801140413398	27.517224994060346	26.300784034212406
10-11	28.823948681397006	20.4727963886909	27.62176288904728	23.081492040864813
12-13	25.31717747683535	26.904252791636967	29.807555238774057	17.971014492753625
14-15	20.915203497350852	28.59416949796859	31.72800494190881	18.762622062771744
16-17	22.691850795913517	28.923734853884532	29.529579472558805	18.854834877643146
18-19	24.126870990734144	26.97077690662865	28.572107388928487	20.33024471370872
20-21	26.17248752672844	24.309812306961273	31.195058208600617	18.32264195770967
22-23	27.184604419101927	24.352577809455926	29.090045141363746	19.3727726300784
24-25	25.323132484318574	27.547044288158144	26.340049420262307	20.78977380726098
26-27	21.374263448013686	27.47814103782551	25.81495913324463	25.332636380916174
28-29	22.129823227523286	24.895457137426344	26.20937084204524	26.765348793005135
30-31	22.714312868275993	25.109294810872456	28.80631058734081	23.370081733510737
32-33	24.040677643928056	28.923420533656476	28.906788319433552	18.12911350298192
34-35	21.771051130963695	32.546093898498384	29.787587911043527	15.895267059494392
36-37	19.43071659380346	30.752233415700438	33.862383577266684	15.954666413229424
38-39	19.65736824597253	34.93560804067861	29.575155633702423	15.831868079646439
40-41	22.2032126223743	34.74004372207965	24.448721604410228	18.608022051135823
42-43	21.538900242383917	34.31395846205028	25.4027850387339	18.7443562568319
44-45	19.21916213197728	34.957108571157	29.33963833376898	16.484090963096737
46-47	19.14120051328359	34.13335868067107	30.88493892875814	15.840501877287203
48-49	21.770437262357415	33.63593155893536	28.436311787072242	16.15731939163498
50-51	21.031368821292777	35.116444866920155	25.313688212927755	18.538498098859314
52-53	22.6793098531299	34.72598507533628	22.572365606730358	20.02233946480346
54-55	26.416484456697404	33.41810057990303	21.78914345470102	18.376271508698547
56-57	27.02792632204397	35.695781342840164	21.846702317290553	15.429590017825312
58-59	25.87891321400556	38.66980436901281	21.005966388552142	14.445316028429486
60-61	21.598097502972653	43.18430439952438	21.265160523186683	13.95243757431629
62-63	19.471554391811473	45.78671744822661	21.061651987621996	13.68007617233992
64-65	18.769098548510314	46.79144385026738	20.63120702826585	13.808250572956455
66-67	19.291140453880995	47.16863572096145	20.50851925519423	13.031704569963335
68-69	20.271147328189226	46.69358909641595	20.35287613278527	12.682387442609553
70-71	20.34131173620652	46.86769349434763	20.256947959601803	12.53404680984405
72-73	20.029027576197386	47.43831640058055	19.796806966618288	12.735849056603774
74-75	20.58551380249833	46.01860895491698	20.6806148151956	12.715262427389092
76	32.62632563942607	0.0	39.44687045123727	27.926803909336662
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	1.0
6	1.0
7	3.0
8	4.0
9	6.5
10	14.0
11	13.5
12	9.0
13	21.0
14	35.5
15	46.5
16	53.5
17	67.0
18	90.5
19	119.5
20	163.5
21	215.5
22	258.0
23	299.0
24	332.0
25	376.5
26	458.5
27	531.5
28	561.0
29	577.5
30	610.5
31	640.0
32	658.5
33	685.0
34	745.0
35	820.0
36	890.0
37	898.5
38	838.5
39	841.0
40	898.0
41	1199.5
42	1617.0
43	1501.0
44	1204.5
45	937.5
46	642.5
47	531.5
48	507.0
49	498.0
50	460.0
51	432.5
52	436.0
53	425.5
54	396.5
55	380.0
56	336.5
57	328.5
58	341.5
59	321.0
60	312.0
61	318.5
62	315.5
63	283.0
64	225.5
65	187.5
66	172.0
67	146.5
68	110.0
69	80.5
70	62.0
71	54.0
72	49.0
73	45.0
74	46.5
75	44.0
76	29.5
77	17.0
78	11.0
79	6.0
80	6.0
81	6.5
82	7.0
83	4.5
84	1.5
85	1.0
86	0.5
87	0.0
88	0.0
89	0.0
90	0.5
91	1.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.004751722499406035
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0023758612497030173
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0023759741494012547
34-35	0.0
36-37	0.0
38-39	0.00237603060327417
40-41	0.0
42-43	0.004752399961980801
44-45	0.0023761999809904003
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.005140331037318804
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
22-23	1.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	2.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	2.0
48-49	0.0
50-51	0.0
52-53	2.0
54-55	0.0
56-57	2.0
58-59	10.0
60-61	13.0
62-63	49.0
64-65	76.0
66-67	79.0
68-69	50.0
70-71	56.0
72-73	517.0
74-75	10568.0
76-77	9618.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.52551186208683
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.98412320663361	86.82553272569568
2	1.6617130277208747	2.9753141874921876
3	0.47664813915940335	1.2801650590907032
4	0.2873985350054305	1.0291800381910017
5	0.12470244054651011	0.5582024910188885
6	0.10441786633022423	0.5608837758456173
7	0.08098213161521745	0.5074976749174658
8	0.03205991313413549	0.2296144106870016
9	0.02124463039416831	0.17117427693228887
>10	0.20038152295025805	3.6090667042979008
>50	0.021062869208110076	1.1455409693875218
>100	0.005265717302022215	1.1078276864437764
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	235	1.116654787360418	No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAAA	88	0.418151579947731	No Hit
AGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	52	0.24708956996911383	No Hit
TCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	52	0.24708956996911383	No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	51	0.24233784746970777	No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	49	0.2328344024708957	No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	46	0.2185792349726776	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAAAAAAAAAAAAAA	44	0.2090757899738655	No Hit
GGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGC	39	0.18531717747683535	No Hit
GCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	39	0.18531717747683535	No Hit
GGGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTG	36	0.17106200997861726	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	35	0.1663102874792112	No Hit
AAGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	27	0.12829650748396293	No Hit
GGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA	25	0.11879306248515087	No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAAAAAAAAAAAAAAA	25	0.11879306248515087	No Hit
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAA	24	0.11404133998574484	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10	0.0	0.0	0.0
11	0.0	0.0	0.0
12	0.0	0.0	0.0
13	0.0	0.0	0.0
14	0.0	0.0	0.0
15	0.0	0.0	0.0
16	0.0	0.0	0.0
17	0.0	0.0	0.0
18	0.0	0.0	0.0
19	0.0	0.0	0.0
20	0.0	0.0	0.0
21	0.0	0.0	0.0
22	0.0	0.0	0.0
23	0.0	0.0	0.0
24	0.004751722499406035	0.0	0.0
25	0.00950344499881207	0.0	0.0
26	0.00950344499881207	0.0	0.0
27	0.00950344499881207	0.0	0.0
28	0.01900688999762414	0.0	0.0
29	0.03801377999524828	0.0	0.0
30	0.06177239249227845	0.0	0.0
31	0.08077928248990258	0.0	0.0
32	0.1520551199809931	0.0	0.0
33	0.16155856497980517	0.0	0.0
34	0.18056545497742932	0.0	0.0
35	0.19957234497505347	0.0	0.0
36	0.2185792349726776	0.0	0.0
37	0.2613447374673319	0.0	0.0
38	0.27084818246614395	0.0	0.0
39	0.2898550724637681	0.0	0.0
40	0.3041102399619862	0.0	0.0
41	0.3421240199572345	0.0	0.0
42	0.3848895224518888	0.0	0.0
43	0.413399857448325	0.0	0.0
44	0.4276550249465431	0.0	0.0
45	0.4371584699453552	0.0	0.0
46	0.4609170824423854	0.0	0.0
47	0.5131860299358517	0.0	0.0
48	0.5559515324305061	0.0	0.0
49	0.5892135899263483	0.0	0.0
50	0.6367308149204086	0.0	0.0
51	0.7127583749109052	0.0	0.0
52	0.7697790449037776	0.0	0.0
53	0.817296269897838	0.0	0.0
54	0.8790686623901164	0.0	0.0
55	0.9360893323829889	0.0	0.0
56	1.0026134473746733	0.0	0.0
57	1.0738892848657637	0.0	0.0
58	1.1214065098598243	0.0	0.0
59	1.1784271798526966	0.0	0.0
60	1.249703017343787	0.0	0.0
61	1.3019719648372534	0.0	0.0
62	1.3399857448325019	0.0	0.0
63	1.35424091233072	0.0	0.0
64	1.35424091233072	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGTCC	35	0.0027071321	66.1003	70
CGCGACC	130	4.5318848E-6	21.24249	3
GAATTTA	335	0.0	19.671642	34
ATATTAG	335	0.0	19.671642	44
CGCTGAA	335	0.0	19.577969	30
CCGCTGA	335	0.0	19.577969	29
CCCGCTG	335	0.0	19.577969	28
CGTGGCG	335	0.0	19.577969	20
AGACGTG	335	0.0	19.577969	17
GACGTGG	335	0.0	19.577969	18
TCAGACG	335	0.0	19.577969	15
CAGACGT	335	0.0	19.577969	16
TGGCGAC	335	0.0	19.577969	22
ACGTGGC	335	0.0	19.577969	19
ACCTCAG	300	0.0	19.560795	5
GACCTCA	265	3.6379788E-12	19.539083	6
GGAGGAA	285	0.0	19.425365	54
AATTTAA	340	0.0	19.382353	35
GCATATT	340	0.0	19.382353	42
TTTAAGC	340	0.0	19.382353	35
>>END_MODULE
