FastQCFastQC Report
Wed 15 Feb 2017
OU_LexBC001_S41_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOU_LexBC001_S41_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42
Sequences flagged as poor quality0
Sequence length73-76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGGCGATAATGTCTCTAAAGATTTCAAAGTCATATGAGATTTGTGATAT12.380952380952381No Hit
TGGACTCAGCTCTGTCTGCTCAAAGCCATTGTGAAGAGAAGCACCCTAATGCATAAGCAATATAAAGCTGTAAAA12.380952380952381No Hit
TACCACGCTACTCCTACCTATCTCCCCAATTATACTAATAATCTTATAAAAAAAAAAAAAAAAAAGAACTCAAA12.380952380952381No Hit
GATTGTGGTGCTGGGGTGTTTATGGCAAGTCACTTTGACAGACATTATTG12.380952380952381No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA12.380952380952381No Hit
GCCCTGGATCTCTAACATTCTGGACTATTTCTGTGTTTAATTGTCGCCGA12.380952380952381No Hit
AGAGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAAAAAAAACAAAAAAAGGAAGGGGGGAGG12.380952380952381No Hit
CACGATCAAGAAACCATATCAACCTCAAAATGTCAACACTAACAGGAGAAATAGACAAAAATTACTATCAGAATC12.380952380952381No Hit
TTTTCCCCTTTTAAGGTTTCAACAAATTTAATTGAACTTTCATTTCAATAAAGTTGTTGCATTCCCAAAAAAAAA12.380952380952381No Hit
GGGAGTCTGATGATGACATGGGCTTTGGTCTTTTTGACTAAACATCTTTT12.380952380952381No Hit
TACCTCAGCGCGAGCGCCAGGCGTCCGGCCGCCGTGGCTAGGTTCGTCTC12.380952380952381No Hit
TCACTTGGCAATTTAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAA12.380952380952381No Hit
CCAGAGAAGCTCAGAAGGCTAAATGAATATTATCCCTAATACCTGCCACC12.380952380952381No Hit
TGTTTGCCTGGGCCCAGGATTGGAGGGCTTCACACCAATAGCCTGTGTAT12.380952380952381No Hit
GTTGGGAAAGATAATTTCCTATTATAAAATATCAAATTTTAAATAAAGATAATGAAAGAAAACATAAAAAAAAAA12.380952380952381No Hit
ACCGAGGAGTCTGATGATGACATGGGCTTTGGTCTTTTTGACTACCCCTC12.380952380952381No Hit
TGGGAATAAGCTTGTGTATCCATTATCATGTGTAATCAATAAACGATTTAATTCTCTTAAAAAAAAAAAAAAAAA12.380952380952381No Hit
GCTAGTGGGCAAAGAGTTGGAGTTCTATCTTAGGAAAATCAAGGCCCGCA12.380952380952381No Hit
CTGTAATCTTGCACATGCCAACAAGGTGGTTCTACAAGGTATACTTATAGTCTCAAAACACACAATTACTTTAC12.380952380952381No Hit
GTGGGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTG12.380952380952381No Hit
AGGAAGCCCTTGTTGCTGTAGATGTGCGTGCAGTCTGGCAGCCTTAAGCCCACCTGGGCACTTTTAGATAAAAAA12.380952380952381No Hit
GGAAGGATCTGATCTCTGAAGCTGCGGTCACCACCCTCAAGTTGTGGCCT12.380952380952381No Hit
GGGTTTGGTGGACAGTTTTTGTTATGATGTATGAGTGGAAAGCGGCTGTGCAGACATTCAATTGTTATAAAAAAA12.380952380952381No Hit
GATCGCTTGAACCCTGGAGGCGGAGGTTGCAGTGAGCAAAAATCGCGCAA12.380952380952381No Hit
ACTGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAA12.380952380952381No Hit
GCTCGGGGTTAGTGAAACATTAAGAAAGAAAAAAAAAAAAAAAAAAAAAA12.380952380952381No Hit
CGATACATCAGATCAGAAGTGTCAACACTCTTAATTTAAGCATCTGAGTCAGACGAGTAAAAAAAAAAAAAACAC12.380952380952381No Hit
TGTAGGGTGTGGCATTCATTGTAGATGTAAGCTTATTTTTCTGTGAGCAA12.380952380952381No Hit
AAACTAGGATCATCTGTTACAAACAAGGTGGTACTCCAAGAGATACAAATAATATCAAGACACAAACTTCATTTA12.380952380952381No Hit
GGGAGTGTGAAGATTTACTGACATCAAAAGTTCTTCTGGAAACAAACCTT12.380952380952381No Hit
ACATTGCTTCACATGCACCGTAACTGGAAGAGGAGCACAGAGTGTGCTCA12.380952380952381No Hit
CAGTGTGCGCTGCAGTGGCCTCGTTTTTATAACTCTTTGTCAGAATTGAACTCAATATTGCTAAAAAAGAAACTA12.380952380952381No Hit
AGGGGGGAGACTGTGTGAGTCCGTGGTTTCAGGCTGCAGTGAACCATGAT12.380952380952381No Hit
TGGTGGTACTTTTGTCTCTCTGCAAAAAATTAAAAAAATAAGAGTAGTAGGGAGAGAACAGAGGGTGTCGGTGC12.380952380952381No Hit
GTCTCTATGTGAATATGGACAGTTAGCATTTACCAACATGTATCTGTCTACTTTCTCTTGTTTTAAAAAAAAAAA12.380952380952381No Hit
CGTTTACTGAGAAAAAATGAGAGTGTTCAAAGCAGGCCCGAGACGACTGGATACAGCAGCTAGGTAAAATGGAAT12.380952380952381No Hit
CGAAAAAAAAAAAAAAAAAAAATAAAAATTAATAAAAAAAAAAAAAAAAC12.380952380952381No Hit
ATTTGGTGGAAATTTTTTGTTATGATGTCTATGTGGAAAGCGGCTGTGCAGACAATCAAATGTTAGAGAAAAAAA12.380952380952381No Hit
GCGGTAGAAATTGAAACCTGGCGCAATAGATATAGTACAGCAAGGGAAAAAAAAAAAAAAAAAAGAAAGGAAA12.380952380952381No Hit
TACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAA12.380952380952381No Hit
TTAGGAGGTTGGAGGTGTAGCGATGGAGAATGAAACTGGAGTTGAGGAGG12.380952380952381No Hit
CAAGGAGTCTCAGGCCAAGGATGTCATTGAAGAGTATTTCAAATGCAAGA12.380952380952381No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)