FastQCFastQC Report
Wed 15 Feb 2017
OU_LexBC001_S41_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOU_LexBC001_S41_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length74-76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGGTGCTGAGAACCCAGCAATGACCAGGAAGATACAGTCACTAACTT18.333333333333332No Hit
AGCTGTACCAGAGGCCTTTATGTGCTACACATAATTAGTATAAAATTTTATATGTGCAGATTGGGTACATAAAC18.333333333333332No Hit
TGCGGAACTTTATCCGGACCCAGCATGTAGGTCCCGGGAGACTGCCATGA18.333333333333332No Hit
AGCTGTTAGTACACATGGATGAACGACGAGAAGAACAAATTGTACAGTTACTGAATTCTGTTCAAGCGAAGAATG18.333333333333332No Hit
CCCCGCGTAAATGAGATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTATGATAAAAAAAAA18.333333333333332No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAAA18.333333333333332No Hit
CAGCGAGCTCAGATCAGACGTGGCGACACGCTGAATTAAAGCATAATTGTCAGCGGAGGAAAAAAAAAAAAAAAA18.333333333333332No Hit
CAAACTTATTGCACTAAATACAGGATCTGTACAAAGAAAAAAAAAAAAAAAAAAAAAAGAAGAGAGAGAACAATT18.333333333333332No Hit
GAACGCGACCTCAGATCAGACGTGGCGACCAGCTGAATTTAAGCATATTAGTCAGCTGAGGTAAAAAAAAAAAAA18.333333333333332No Hit
CGGGGCGCAACCACACCTTCCTTAGGTTGATGTGCTTGGGAAAGCTCGCT18.333333333333332No Hit
CAGGCGTGAGCCACCATGTCCGGCCTGTTTTTTCCTATGCATGCATAATT18.333333333333332No Hit
TGTGTTTTCCTCTCTGAATCTGTTATGAACACGTTGGTTGTCTGGATTCA18.333333333333332No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)