FastQCFastQC Report
Wed 15 Feb 2017
OU_LexBC001_S41_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOU_LexBC001_S41_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length73-76
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGTGTGTGAGATGTATTAAAATAATCAAAGTTGAAGTCAAACATCTT13.225806451612903No Hit
TGTGGCAGTTACTAACTTTCTACAGTTTTCTTAATCAAAAGTGGTCTAGG13.225806451612903No Hit
CGCGACCTCAGATCAGACGTGGCGAACAGCTGAATTTAAGCATATAAGTCAGCGGAGGAAAAAAAAAAAAAAAAA13.225806451612903No Hit
ATGGAAGCTGATGGGGTTGAAGTCAAAAGACCAAAATACTAATCACTAGT13.225806451612903No Hit
ATTTTGTAGTCATAGTCTAATATAAGAACAAAAAAAAAAAAAAACTAAGAGGATAAAAAAACAAAAAAAAATAAC13.225806451612903No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAATAATAAAATATTATAGGAGGG13.225806451612903No Hit
AGCGGCGGAGCAGACATTCAAATGTAAAAAAAAAAAAAAAAAAAAAAAAA13.225806451612903No Hit
ACGCGCACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAAAAAAAAA13.225806451612903No Hit
ATCCACCTCCTTGATTTGGGTACTCTTGGATATGTTCCAGCTGCTTAACTTCAGTGAATGCAACAAATGAGATGA13.225806451612903No Hit
TTCAGGGCATTGAGCAAGTGCAGCAAAGTAGAGGCCTGCAAGAAATAGAA13.225806451612903No Hit
GGGGTGGCAATGTCATGGGTCCTAAGTCACTGGCTCGTATCACAAAGCTA13.225806451612903No Hit
TGTTGTTTTTGTATATTTAAATGCTTACCTTTATTATTTGTAAAGTGCAG13.225806451612903No Hit
TGTTGACGGAGGTAGGTTTCAACCCAGAGTTAATGTCAAGCATGCTAATT13.225806451612903No Hit
GCCTGCCCCTCTTTTTCGGTTTGTTTTTATTCTTTCATTATTACAAGGGACGTTATATAAAGAACTGAACTCA13.225806451612903No Hit
TTTTCTAGTTGATCTCATGCTAGCAGAGCAAAAAATGAAAAATATATTGA13.225806451612903No Hit
TGGCGTGGTGGTAGGCACCTGTAGTCCCAGCTACTATGGAGACTGAGGCA13.225806451612903No Hit
GGGATGAAGTGGGACAAAAAAGATGCAGTTTTCCTTTGTATTGGGAAATGTGAAAATAAAAATGTCAACTCTTTC13.225806451612903No Hit
TTTAAACATTGGAATCTCTTTTAGAAGTGATATAATTTAATGGCATATTG13.225806451612903No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAG13.225806451612903No Hit
TACGCGACATCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGAGGAGGAGAAAAAAAAAAAAA13.225806451612903No Hit
CCTACCTATCTCCCCTTTTATACTAATAATCTTATAGAAAAAAAAAAAAAAAAAAAAAATCGGAAGAGCGGAGGT13.225806451612903No Hit
TGTGGATGAGACTTAACTTAAAACCACTTACAATAAACTTGGGAAACTACCGTCAAAAAAAAAAAAAAAAAAAAA13.225806451612903No Hit
TAATCACCCAGCCAAACACATAACAATAAAAACCAAACTCTGTGTCGAAA13.225806451612903No Hit
ATAACAGGAATATTTTGCAGAATATAGGTTTAACTGAATGAAGCCATATTAATAACTGCATTTTCCTAACTTTGA13.225806451612903No Hit
GAATGGTCTGTCACAATCTGCTCCTTTTTTTTTGTCCGCCACACGTAACT13.225806451612903No Hit
GGGGTTCATAAAAAAAAAAAAAAAAAAATAAGGAAAGAGAGCACAGCGGA13.225806451612903No Hit
GGGAAAAAAAGACAGGAAGTGTTAGAAAAGCAAATAGAATGCCAAAAGATGTTAATATCCAAGTTAAAAAAAAAA13.225806451612903No Hit
TGGAGAGCCGGAGAACTCGGGAGGGAGACGGGGGAGAGAGAGAGAAAAAAAAAAAAAAAAAAAAGATCGGAACAA13.225806451612903No Hit
CGCATGTTGTAATCGTGAAAATAAACGCTCACTCGGAAAAAAAAAAAAAT13.225806451612903No Hit
TTAATGTGTTTAAAAAATTAAAAACAAAAAAAAATTTTAAACACATTAAT13.225806451612903No Hit
TCAAAACAAATTCTATATCAAAAACTTGAAATAAAAAAAAAAAAAAACAA13.225806451612903No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)