FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRKO-T0_S12_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRKO-T0_S12_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5886343
Sequences flagged as poor quality0
Sequence length15-23
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGAT750.036.5505417
TTTCGAT900.030.45878417
TGTCGAT1150.028.94530517
GGCCTAC11000.028.12492817
GTCGTTG356.3676183E-427.97235317
AGCCTAC9450.027.76515217
ACCATCT18000.027.6304717
ACCCTTC20200.027.33535817
AACCTTC21700.026.97978417
TACCTTC10750.026.77539617
CCAACTT14300.026.56395317
AACCTAC6350.026.21031417
GACCTTC23150.025.71281617
GTCCATC42400.025.3070617
CCAACAT13850.025.30639617
TCCCATC54600.025.2468417
ACCCATC41950.025.11176917
CACCTTC29400.025.10851517
TACGTTC2500.025.06322717
ATTCGTT951.382432E-1024.73344817
Produced by FastQC (version 0.11.5)