FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRKO-T0_S12_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRKO-T0_S12_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6087770
Sequences flagged as poor quality0
Sequence length18-23
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAT1250.035.7561717
AGACGAT750.034.7629417
GACGTTC8750.031.07381417
GTCGTTC12100.030.0122817
GCCGTTC15850.029.60881617
TACGTTC4200.028.3779117
GTCGTTG405.68072E-527.93450717
ATCGTTC5150.027.84410517
AGCCTAC8700.027.39936417
GGCCTAC12450.027.37357317
ACCTTCT17200.027.0683217
ACCCTTC28000.026.67080117
CTCGTTC7800.026.50196617
AATCGAT809.076757E-1025.60663217
AACCTTC26800.025.43290917
TTCGTTC6100.025.33949917
AACCTAC6400.025.31564717
CCATCTT16450.025.01935617
GGCGTTC15100.024.78956217
AGCGTTC10700.024.71464217
Produced by FastQC (version 0.11.5)