FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRKO-T0_S12_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRKO-T0_S12_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6044904
Sequences flagged as poor quality0
Sequence length18-23
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAT1000.034.49474717
GTACGAT250.002598810734.49474717
AGACGAT551.6417471E-731.3588617
ACCATCT13350.031.00651617
TTTCGAT502.76651E-630.18290517
GTCCATC33500.029.86112417
CATCGAT801.6552804E-1029.64392517
ACCCATC34000.029.23176217
CGCCAAC106700.027.90372717
CCCCATC42000.027.2570117
AGCCATC36000.027.12868117
AACCATC27200.027.10754417
CGCCATC27150.026.99865217
TACGTTC805.3605618E-926.94902217
TTCCATC26450.026.89807717
TGCCATC39700.026.77252817
GACCATC32500.026.60075617
TCCCATC40200.026.43953517
AGCCTAC6700.026.38590617
ATCCATC22500.026.25433517
Produced by FastQC (version 0.11.5)