FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRKO-T0_S12_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRKO-T0_S12_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5875333
Sequences flagged as poor quality0
Sequence length15-23
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTTG504.5383786E-934.9811517
TACGTTC3300.034.7455917
AGACGAT1200.032.38995417
GTCGTTC9000.031.52622217
GACGTTC8850.030.96260317
GCCGTTC12400.030.87494317
GGCCTAC17150.030.4824417
TACCTAC7750.029.84055117
AGCCTAC12900.029.8288917
TTCGTTC5500.029.68097717
ACCCTTC29950.029.58895517
GACCTTC29350.029.4654817
TCCGTAC2450.029.34926617
GACCTAC13200.029.29818717
GCCCTAC17200.029.15095717
CATCGAT1350.028.79107317
TGTCGAT2100.028.68824617
GTCGTAC1900.028.6395417
TACCTTC15650.028.56111117
TCCCTAC20250.028.3112217
Produced by FastQC (version 0.11.5)