FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRKO-Rep2_S18_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRKO-Rep2_S18_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5881695
Sequences flagged as poor quality0
Sequence length15-23
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGAT900.032.67190617
TGTCGAT1050.031.73842217
ACCCTTC20050.030.11355617
AGCCTAC8500.029.5200317
ACCATCT17900.029.02141417
GACCTTC22850.027.62456117
GGCCTAC10600.027.37042617
TACCTTC12000.026.9543217
CATCGAT805.002221E-1026.9543217
GTCGTTC5000.026.26821117
CCAACTT15400.026.22238517
TTTCGAT757.4269337E-926.13752417
GTCCATC44250.026.09322417
ACCCATC39800.025.46438617
TCCCATC55300.025.41673317
CCAACAT15150.025.10237317
AACCTTC20250.025.07266217
AACCTAC6500.025.03170617
CCCCATC50050.025.02778617
TACGTTC1650.024.94945317
Produced by FastQC (version 0.11.5)