FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRKO-Rep2_S18_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRKO-Rep2_S18_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6096519
Sequences flagged as poor quality0
Sequence length18-23
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTTC3900.031.83494817
GACGTTC9000.031.56161717
GTCGTTC9900.030.0224917
GCCGTTC14400.029.91560717
TGTCGAT1350.029.26242817
TTCGTTC6650.028.28806117
AGCCTAC10050.028.26413517
ATCGTTC5800.028.2173417
ACCCTTC30700.027.88032517
AGACGAT753.8198777E-1027.5902917
GTCGTTG657.997551E-826.04677617
AGCGTTC9550.026.0013217
ACCTTCT17750.025.6473117
GGCGTTC14600.025.1250317
CTCGTTC8050.025.00418717
AACCTTC27550.024.5813517
CCCCTTC44000.024.54053517
CCATCTT15800.024.28834317
GACCTTC29300.024.26883917
ACTCGAT1250.024.07879617
Produced by FastQC (version 0.11.5)