FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRKO-Rep2_S18_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRKO-Rep2_S18_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6062100
Sequences flagged as poor quality0
Sequence length17-23
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAT1000.036.82187717
AGCCTAC6950.030.230417
ACCATCT14000.029.70504417
GTCCATC32150.029.3739617
ACCCATC31350.029.22535717
CCCCATC38650.028.52497717
GCCCATC48950.027.78842417
TCCCATC44100.027.35732317
GACCATC34050.027.28959717
CGCCAAC107550.026.26178217
AGACGAT509.277162E-525.99191317
AACCTTC15500.025.99191317
CACCATC39200.025.9698117
CCCCAAC164000.025.88625517
TTCCATC26950.025.87939517
AGCCATC38250.025.87865617
TCCCAAC180950.025.83151217
CCAACAT11750.025.62323217
ACCCTTC14950.025.49931117
GCCCAAC189600.025.22421817
Produced by FastQC (version 0.11.5)