FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRKO-Rep2_S18_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRKO-Rep2_S18_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5877200
Sequences flagged as poor quality0
Sequence length15-23
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAT2350.034.9025817
TACGTTC4850.033.01780317
AGCCTAC11650.032.3524717
GACGTTC7700.031.70263717
ACCCTTC29450.031.43179117
GTCGTTG757.2759576E-1231.2461217
GGCCTAC18600.030.34317217
AGACGAT1100.030.1809117
GCCGTTC13500.029.79953817
GACCTAC12600.029.75820717
GTCGTTC8900.029.40294617
ACCCTAC11750.029.08548217
AACCTAC9450.028.93159117
GTCGTAC2300.028.86869617
CCCCTAC18200.028.86402917
GCCCTAC19700.028.84714517
GACCTTC27550.028.6375517
CTCGTAC2400.028.47953417
TACCTAC9600.028.47953417
TACCTTC15450.028.44003317
Produced by FastQC (version 0.11.5)