FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRKO-Rep1_S15_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRKO-Rep1_S15_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6931808
Sequences flagged as poor quality0
Sequence length15-23
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGAT800.034.22316417
TGTCGAT1350.033.31778717
ACCATCT21450.029.35693217
ACCCTTC27100.028.79291717
TACCTTC14000.027.51821717
TTTCGAT1000.027.37852917
GGCCTAC12550.027.26945117
AGCCTAC8550.026.76096717
GACCTTC27300.026.71949817
AACCTAC8350.026.4651317
TACGTTC2400.026.07479117
GTCGTTG459.0793474E-526.07478717
GGCCTTC37400.025.36017217
AACCTTC25100.025.16580817
GTCCATC52300.025.16491517
TCCCATC61450.024.91850517
ACCCATC44850.024.89750117
CCCCATC60750.024.78714617
GCCCTAC14050.024.7756917
CGCCAAC102550.024.60005817
Produced by FastQC (version 0.11.5)