FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRKO-Rep1_S15_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRKO-Rep1_S15_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7186883
Sequences flagged as poor quality0
Sequence length15-23
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGAT559.094947E-1237.7302717
TACGTTC5800.031.87557617
GTCGTTC11900.029.80374517
GCCGTTC17600.029.58396117
GACGTTC10050.029.4709117
TTCGTTC7350.029.26021217
AGCGTTC11250.028.50731717
ACCTTCT21450.027.44019917
ATCGTTC6450.026.90841317
AGCCTAC11100.026.6831217
TGTCGAT1500.026.4111917
CTCGTTC9450.026.1516717
TACCTAC7650.025.64671917
GGCCTAC14050.025.64584417
GACCTAC10250.025.39891617
GGCGTTC17250.025.37224217
ACCCTTC33250.025.30481317
CCATCTT19900.024.74271817
AACCTAC8750.024.57857717
AACCTTC34050.024.54406717
Produced by FastQC (version 0.11.5)