FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRKO-Rep1_S15_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRKO-Rep1_S15_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7139971
Sequences flagged as poor quality0
Sequence length16-23
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAT751.8189894E-1234.6079217
AGCCTAC8100.031.24326517
ACCATCT14550.031.0698317
ACCCATC38450.030.71509617
AGACGAT454.570732E-528.83993717
TCCCATC47850.028.11667817
GACCATC37900.027.79363417
GTCCATC41300.027.39095717
AACCTTC17400.027.22390717
ACCCTTC17250.027.20985617
CGCCAAC128950.027.10663417
AGCCATC40400.027.0909817
CGCCATC32850.026.99628617
CCCCATC48600.026.70364417
GCCCATC58150.026.48413717
AACCATC32700.026.4586617
TTCCATC33700.025.99445517
CCAACTT10250.025.95594417
GGCCATC56950.025.7888317
TACCATC20150.025.76272417
Produced by FastQC (version 0.11.5)