FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRKO-Rep1_S15_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRKO-Rep1_S15_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6925066
Sequences flagged as poor quality0
Sequence length15-23
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTTG555.456968E-1239.6660217
AGCCTAC17200.033.3240717
GTCGTAC2100.033.05501617
GGCCTAC20150.032.0871517
AATCGAT1750.031.73281717
TGTCGAT2400.031.40226617
TACGTTC4650.031.13569317
ACCCTTC34300.030.9348717
GACGTTC8750.030.82616417
GGCGTAC3750.030.67505617
GTCGTTC11850.030.46082517
AGACGAT1500.030.41061417
TACCTAC10550.030.078517
AACCTTC34050.029.93881417
TCCCTAC22250.029.86093517
GCCCTAC21200.029.84306717
TACCTTC18750.029.7230717
GACCTAC14500.029.54434617
TGCCTAC23850.029.5208317
CCCCTAC21750.029.36197517
Produced by FastQC (version 0.11.5)