FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRISPRi-T0_S13_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRISPRi-T0_S13_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7905604
Sequences flagged as poor quality0
Sequence length18-23
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGAT900.038.65941217
TGTCGAT1800.031.14230517
TTTCGAT1100.029.87318417
CCAACTT17050.027.66245317
AGCCTAC11100.027.16607517
GGCCTAC16100.027.13362717
ACCCTTC28350.026.65931317
AACCTTC29050.026.61577617
ACCATCT22500.026.54613317
GTCCATC62900.026.45926317
ACCCATC54500.026.06850417
TACCTTC15150.025.90053217
GACCTAC11300.025.48784417
CGCCATC45450.025.39017717
AACCTAC9200.025.21266217
CGCCAAC117950.025.04094317
GACCATC56000.024.74892617
GCCCTAC16000.024.64537817
GACCTTC31150.024.32502617
CCAACAT16750.024.1188617
Produced by FastQC (version 0.11.5)