FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRISPRi-T0_S13_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRISPRi-T0_S13_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8191466
Sequences flagged as poor quality0
Sequence length16-23
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGAT1200.032.38767217
GACGTTC12300.031.44726617
GTCGTTC15200.031.2918517
GCCGTTC18500.030.51194217
TGTCGAT2450.029.46050817
TACGTTC5450.029.20408817
ACCCTTC43550.027.41026717
ATCGTTC6200.026.8653517
GGCCTAC15800.026.35525117
TTCGTTC9350.026.3258117
AGCCTAC13400.025.96538417
GTCGTTG809.767973E-1025.44745817
GCCCTAC18650.025.20557417
AACCTAC10450.025.14859817
CTCGTTC12250.024.92812317
AGCGTTC11600.024.72950617
GACCTTC44150.024.64837617
CCATCTT19800.024.3024417
ACCTTCT21900.024.16927517
CGCCAAC108550.024.07390817
Produced by FastQC (version 0.11.5)