FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRISPRi-T0_S13_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRISPRi-T0_S13_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8135693
Sequences flagged as poor quality0
Sequence length17-23
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGAT501.9699655E-938.09760717
ACCATCT17250.030.06091517
ACCCATC46350.029.72736717
GTCCATC47050.029.1051517
CGCCAAC140750.028.16531417
ATTCGTT1003.6379788E-1227.51493817
CCCCATC60250.027.43589817
TACCTTC11200.027.2125817
AGCCATC48250.027.06531117
CCCCAAC232750.026.91703617
AACCATC34700.026.59391217
CACCATC54500.026.40812917
CGCCATC35100.026.23054517
TCCCAAC239250.026.19459317
TGCCATC56350.025.8416217
ACCCTTC19600.025.80875417
TCCCATC57900.025.69815817
ACCCAAC165000.025.60364517
AGCCTAC8450.025.54869517
GGCCATC66000.025.5266817
Produced by FastQC (version 0.11.5)