FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRISPRi-T0_S13_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRISPRi-T0_S13_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7898267
Sequences flagged as poor quality0
Sequence length15-23
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGAT1100.033.20035617
TACGTTC5000.031.52286117
GTCGTTC11500.031.25543617
TCCGTAC3700.031.16960517
AGCCTAC18550.030.98197217
GGCCTAC24200.030.658717
AACCTAC14450.030.19533217
AGCGTAC3250.030.16258817
TGTCGAT3000.030.11330217
ACCCTTC40800.030.1038817
GACGTTC10800.029.89973317
GACCTAC18000.029.8997317
TACCTAC11300.029.25713517
AACCTTC36800.029.19750817
GCCGTTC17350.029.13654717
TTTCGAT1800.028.83188617
GTACGAT404.5797766E-528.83188417
CTCGTTC10250.028.50371717
GGCCTTC55650.028.46058717
GTCGTTG1150.028.41403217
Produced by FastQC (version 0.11.5)