FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRISPRi-Rep2_S19_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRISPRi-Rep2_S19_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6245796
Sequences flagged as poor quality0
Sequence length18-23
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGAT850.034.0707917
TGTCGAT1650.029.25269917
CCAACTT12450.028.37864117
TACCTTC12400.027.55887217
ACCATCT18300.026.79739217
TACCTAC6350.026.75585217
GACCTAC8750.026.6985217
GTCCATC48800.026.66551217
TTTCGAT955.456968E-1226.41980617
ACCCATC44900.026.10070417
AACCTAC7200.025.74237417
AGCCTAC9250.025.672817
ACCCTTC24450.025.34755317
CCCCATC57350.024.77731517
AACCTTC21250.024.62182417
AGCCATC47650.024.51333217
GGCCTTC33850.024.35449217
GACCTTC25500.024.3038317
CGCCAAC88050.024.29518717
CGCCATC35400.024.26982317
Produced by FastQC (version 0.11.5)