FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRISPRi-Rep2_S19_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRISPRi-Rep2_S19_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6468578
Sequences flagged as poor quality0
Sequence length17-23
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGAT750.034.4851317
TGTCGAT1600.032.3298117
TACGTTC4550.031.67001717
GACGTTC9000.029.76395217
GTCGTTC10750.029.55868317
GCCGTTC14600.029.10315517
CTCGTTC9000.027.71126617
GACCTAC9300.027.41329417
ATCGTTC4900.027.1457317
AGCCTAC10650.026.54036717
GTCGTAC1050.026.3916817
TTCGTTC7150.026.3547717
ACCCTTC33000.026.14375917
GGCGTAC2250.025.4533117
GGCGTTC13950.025.42682517
ACCTTCT20000.024.9401417
AGCGTTC9650.024.88749317
AACCTAC8050.024.7852317
CACGTTC14950.024.71461917
TACCTTC15900.024.39985517
Produced by FastQC (version 0.11.5)