FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRISPRi-Rep2_S19_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRISPRi-Rep2_S19_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6429793
Sequences flagged as poor quality0
Sequence length17-23
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGAT607.2759576E-1238.67749417
TGTCGAT1000.033.75490617
ACCATCT13400.029.59851617
GTCCATC36750.029.50683817
ACCCATC33150.028.76549117
GACCATC36700.027.93747317
CCCCATC47000.027.4258617
AACCTAC4850.027.4041117
CGCCAAC112150.027.37036917
CGCCATC27050.027.06319617
GACCTAC6950.026.71251517
AGCCATC38700.026.7117317
GGCCATC53700.026.59691617
GCCCATC53200.026.48998617
CCCCAAC180450.026.32863817
CACCATC42800.026.22314517
CATCGAT658.5580905E-725.96531117
TTCCATC30150.025.9599317
ATCCATC26500.025.95306417
CCAACTT8300.025.92620717
Produced by FastQC (version 0.11.5)