FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRISPRi-Rep2_S19_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRISPRi-Rep2_S19_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6240089
Sequences flagged as poor quality0
Sequence length15-23
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTTG451.7753337E-938.50575317
AGACGAT1200.032.08812717
AGCCTAC14300.031.77397517
GCCGTTC13850.031.55525417
GTCGTTC9600.031.48647517
CTCGTTC7950.031.48268917
TGTCGAT2400.031.28592317
ACCCTTC31900.031.14258417
GACGTTC8500.031.03110717
GGCCTAC18100.030.9535217
GACCTAC13950.030.5009717
TACGTTC3550.030.37073317
TTTCGAT1350.029.94891517
GTCGTAC1100.029.75444217
AACCTTC27500.029.40439417
CCCCTAC23750.029.18330817
TACCTAC6600.029.17102417
GCCCTAC19650.029.09976817
CATCGAT1350.028.52277817
AACCTAC11100.028.44569217
Produced by FastQC (version 0.11.5)