FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRISPRi-Rep1_S16_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRISPRi-Rep1_S16_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5177667
Sequences flagged as poor quality0
Sequence length15-23
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTTG200.001519181838.48153717
TGTCGAT1150.031.78909717
AGACGAT900.029.93008617
ACCATCT15400.029.73573517
GCTCGAT1400.028.86115517
CCAACTT10700.028.59142517
GGCCTAC11800.027.55669417
AGCCTAC7500.026.68053217
CATCGAT656.426126E-826.64106617
TACGTTC1950.026.64106417
TACCTTC9850.026.17525917
ACCCTTC21300.026.10602217
GTCCATC40550.026.04977217
AACCTTC18050.026.00968417
TTTCGAT908.731149E-1125.6543617
AATCGAT451.01390535E-425.6543617
GACCTAC6500.025.45701817
GACCTTC20650.025.43694917
AACCTAC5750.025.43127817
TCCCATC48500.025.35021217
Produced by FastQC (version 0.11.5)