FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRISPRi-Rep1_S16_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRISPRi-Rep1_S16_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5369674
Sequences flagged as poor quality0
Sequence length18-23
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGAT700.034.6842517
TGTCGAT1300.034.4790217
TACGTTC4200.033.7949117
GACGTTC7450.031.0851117
GCCGTTC13250.030.44562317
ATCGTTC4550.029.9639117
TTTCGAT751.2732926E-1129.88181517
ATCGCAT405.5712437E-528.01420417
GTCGTTC7950.027.95547517
CTCGTTC7250.027.56339617
AGCGTTC8550.027.52272417
AGCCTAC7650.027.09870517
ACCCTTC26800.026.1326517
AACCTAC6250.025.99717917
GGCCTAC12500.025.8477717
AACGTTC6950.025.79725317
GGCGTTC12700.025.58777617
TTCGTTC5800.025.43818317
CGCCAAC69650.024.85682117
ACCTTCT16650.024.78934317
Produced by FastQC (version 0.11.5)