FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRISPRi-Rep1_S16_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRISPRi-Rep1_S16_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5338652
Sequences flagged as poor quality0
Sequence length18-23
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGAT453.3644028E-837.55513817
TGTCGAT1100.036.48822817
TTTCGAT503.2418866E-629.5746717
ACCCATC29600.029.33202217
GTCCATC29700.029.23326117
TACCTTC6750.029.1052317
ACCATCT10700.029.02187217
AGCCATC29900.028.18990317
CGCCATC23250.027.89377617
CCCCATC38650.027.76553717
AGCCTAC5350.027.24502217
AACCATC23550.027.08993117
CACCATC35700.026.86454617
GACCATC28100.026.53751617
CGCCAAC92200.026.53197317
CCCCAAC148250.026.37567517
GCCCATC45450.026.1212717
ACCCAAC106500.025.92494617
ATCCATC23050.025.84461617
CACCGAT908.240022E-1025.81915717
Produced by FastQC (version 0.11.5)