FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRISPRi-Rep1_S16_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRISPRi-Rep1_S16_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5177847
Sequences flagged as poor quality0
Sequence length15-23
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCAT200.001526725138.4427717
AGACGAT900.036.3070617
GACGTTC6600.033.20057317
TACGTTC3950.031.63012917
GGCCTAC16900.031.50487717
GACCTAC12650.031.1492817
GCCGTTC12000.031.07457517
AGCCTAC12050.030.7861217
AATCGAT1450.030.48909617
AACCTAC9350.030.21971717
GTCGTTC8250.030.05525817
ACCCTTC26550.030.04472717
TACCTAC8050.029.84687217
CATCGAT1050.029.2897317
GCCCTAC15950.029.0429717
TTTCGAT1400.028.8320817
GTCGTTG404.5788638E-528.83207717
TACCTTC13350.028.79608317
TCCCTAC18700.028.78068417
AACCTTC24250.028.77262917
Produced by FastQC (version 0.11.5)