FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRISPRa-T0_S14_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRISPRa-T0_S14_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6188609
Sequences flagged as poor quality0
Sequence length15-23
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAT1200.033.73666417
AGACGAT701.2914825E-1030.29414717
TACCTTC12400.028.60620317
ACCATCT17300.028.30425517
ACCCTTC24100.027.75725417
CCAACTT12700.027.62687517
AGCCTAC8200.027.50654617
GTCCATC49400.026.69274517
CATCGAT806.0754246E-1026.50737817
AACCTAC7450.026.3941717
TACGTTC2350.025.43067617
ACCCATC44500.025.2564717
AACCTTC20300.025.16598517
CGCCAAC94400.025.0982817
GGCCTAC11400.025.02770217
GACCTTC26700.024.90989717
CGCCATC35900.024.75543417
GGCCTTC31100.024.67100317
TCCCATC59000.024.440717
GACCTAC9200.024.30716317
Produced by FastQC (version 0.11.5)