FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRISPRa-T0_S14_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRISPRa-T0_S14_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6422992
Sequences flagged as poor quality0
Sequence length17-23
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTTC10300.031.28753517
TACGTTC5250.031.04435717
GTCGTTC10900.029.735217
ATCGCAT250.005467602529.63325317
GCCGTTC15500.029.03580517
CTCGTTC8300.028.33902717
TTCGTTC6700.027.3665317
AGCGTTC10550.026.50841717
ATCGTTC5900.026.36857217
AGCCTAC9900.026.00392717
TTTCGAT1000.025.92909617
GGCCTAC12200.025.65583817
AGACGAT809.677024E-1025.46607617
ACCTTCT17500.025.39993117
ACCCTTC32750.025.22219517
GGCGTTC14550.024.94895717
AACCTAC7800.024.93182217
CGCCAAC84950.024.81065417
GACCTAC8900.024.55564317
TACCTTC17150.024.298417
Produced by FastQC (version 0.11.5)