FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRISPRa-T0_S14_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRISPRa-T0_S14_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6372026
Sequences flagged as poor quality0
Sequence length16-23
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGAT650.039.14438217
TGTCGAT1150.036.8751417
TTTCGAT850.034.9229317
GTCCATC36400.029.12528617
ACCATCT11800.028.39073617
CGCCAAC113200.028.2272417
ACCCATC33050.028.16401917
GACCATC33800.027.97819717
CCAACTT9450.027.822217
CCCCATC49200.027.7968917
AACCATC26750.027.74251617
AGCCTAC6550.026.86818717
TCCCATC46500.026.81179617
TCCCAAC187600.026.7526617
ATCCATC28000.026.65546217
CCCCAAC181250.026.56688917
AGCCATC35650.026.28840817
GGCCATC52100.026.08686317
TACCATC17950.025.63284717
CTCCAAC186000.025.55784417
Produced by FastQC (version 0.11.5)