FastQCFastQC Report
Wed 3 Aug 2016
Carlos3-CRISPRa-T0_S14_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCarlos3-CRISPRa-T0_S14_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6179402
Sequences flagged as poor quality0
Sequence length15-23
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAT2750.033.4193917
TACGTTC4700.032.58983217
GTCGTTG655.820766E-1132.40181417
GACGTTC7600.031.23906917
AGCCTAC14450.031.00544717
GTCGTTC9450.030.99913817
GGCGTAC4000.030.63444117
GGCCTAC19150.030.5944517
GACCTAC13000.030.0453217
ACCCTTC32650.029.14494117
CCCCTAC21250.029.01261717
CTCGTTC7350.028.65466517
TACCTAC9200.028.51167717
TACCTTC16300.028.42612817
AACCTTC26750.028.41559217
GTCGTAC1550.028.41097617
TCCGTAC2900.028.38967717
GCCGTTC14050.028.20876117
GCCCTAC20850.028.19176917
TTCGTTC6400.028.12146217
Produced by FastQC (version 0.11.5)